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IGVFDS9527RJVT
IGVFDS9527RJVT
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This object is in preview status. Metadata is subject to change until this object is released.
File Set Type
experimental data
Summary
CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting genes in many genomic loci
Assay Term
in vitro CRISPR screen using single-cell RNA-seq
Sample Summaries
Homo sapiens (female) TeloHAEC cell line modified with CRISPRi Sp-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library
Protocols
https://www.protocols.io/view/systematic-perturb-seq-to-discover-endothelial-cel-261ged73yv47/v1
Aliases
jesse-engreitz:measurement_set-scRNAseq_2kG_2PMDox1_7
Publications
Convergence of coronary artery disease genes onto endothelial cell programs
Attribution
Jesse Engreitz, Stanford
(
HG011972
—
Jesse Engreitz
)
Sequencing Results (Illumina)
15 items
1
2
Accession
File Format
Content Type
Illumina Read Type
Sequencing Run
Read Names
Associated seqspec Files
Sequence Specification Document
Sequencing Platform
Flowcell ID
Lane
Index
File Size
Lab
Upload Status
IGVFFI0494JWFY
fastq
reads
R2
3
Illumina NovaSeq 6000
5.0 GB
Jesse Engreitz, Stanford
validated
IGVFFI0548FWMT
fastq
reads
R2
2
Illumina NovaSeq 6000
5.3 GB
Jesse Engreitz, Stanford
validated
IGVFFI0695COCF
fastq
reads
R1
4
Illumina NovaSeq 6000
3.9 GB
Jesse Engreitz, Stanford
validated
IGVFFI1187WPAR
fastq
reads
I1
3
Illumina NovaSeq 6000
942 MB
Jesse Engreitz, Stanford
validated
IGVFFI2118ERUN
fastq
reads
R1
1
Illumina NovaSeq 6000
3.7 GB
Jesse Engreitz, Stanford
validated
IGVFFI2973DMIY
fastq
reads
R2
5
Illumina NovaSeq 6000
13 GB
Jesse Engreitz, Stanford
validated
IGVFFI5655LUJS
fastq
reads
R1
5
Illumina NovaSeq 6000
9.5 GB
Jesse Engreitz, Stanford
validated
IGVFFI5981HFKO
fastq
reads
I1
4
Illumina NovaSeq 6000
1.1 GB
Jesse Engreitz, Stanford
validated
IGVFFI6864TNIJ
fastq
reads
I1
5
Illumina NovaSeq 6000
2.8 GB
Jesse Engreitz, Stanford
validated
IGVFFI6903EEOQ
fastq
reads
R2
1
Illumina NovaSeq 6000
5.2 GB
Jesse Engreitz, Stanford
validated
Samples
1 item
Accession
Type
Sample Terms
Associated Construct Library Sets
Summary
IGVFSM7408HYIB
InVitroSystem
TeloHAEC
IGVFDS1298TUYG
Homo sapiens (female) TeloHAEC cell line modified with CRISPRi Sp-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library
Donors
1 item
Accession
Aliases
Sex
Taxa
IGVFDO3459DFTW
igvf:donor_of_teloHAEC
female
Homo sapiens
Associated Construct Library Sets
1 item
Accession
Summary
Library Design Files
IGVFDS1298TUYG
guide (sgRNA) library targeting genes in many genomic loci
IGVFFI9754AGFB
File Sets Using This Measurement Set as an Input
1 item
Accession
Type
Summary
Aliases
Lab
IGVFDS7943IIWZ
AnalysisSet
CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting genes in many genomic loci
jesse-engreitz:analysis_set_TeloHAEC_Perturb-seq_2kG
Jesse Engreitz, Stanford
Auxiliary Datasets
1 item
Accession
Type
Summary
Files
Aliases
Lab
IGVFDS6848XNQV
AuxiliarySet
gRNA sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting genes in many genomic loci
IGVFFI3785OJAM
,
IGVFFI1219TGOD
,
IGVFFI3555NHTT
,
IGVFFI7742QCRG
jesse-engreitz:auxiliary_sets-scRNAseq_2kG_2PMDox1_7
Jesse Engreitz, Stanford