IGVFDS8403DDLC

fastq

reads

I1

1

2

123 MB

Jesse Engreitz, Stanford

fastq

reads

R1

1

1

406 MB

Jesse Engreitz, Stanford

fastq

reads

I1

1

3

124 MB

Jesse Engreitz, Stanford

fastq

reads

R1

1

4

411 MB

Jesse Engreitz, Stanford

fastq

reads

R2

1

4

923 MB

Jesse Engreitz, Stanford

fastq

reads

R2

1

2

912 MB

Jesse Engreitz, Stanford

fastq

reads

R2

1

3

930 MB

Jesse Engreitz, Stanford

fastq

reads

R1

1

2

406 MB

Jesse Engreitz, Stanford

fastq

reads

I1

1

1

123 MB

Jesse Engreitz, Stanford

fastq

reads

I1

1

4

124 MB

Jesse Engreitz, Stanford

InVitroSystem

HCT116 cell line, male, Homo sapiens treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library

male

Homo sapiens

AuxiliarySet

gRNA sequencing for mixed CRISPR TAP-seq integrating a guide (sgRNA) library targeting accessible genome regions in 29 genomic loci

Jesse Engreitz, Stanford

overview of experimental design

experimental protocol