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INDEXED
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MeasurementSet
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IGVFDS8050CYYG
IGVFDS8050CYYG
released
Json view
File Set Type
experimental data
Control Type
unsorted FACS input
Summary
unsorted FACS input CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
Assay Term
in vitro CRISPR screen using flow cytometry
Sample Summaries
THP-1 cell line, male, Homo sapiens modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library
Protocols
https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1
Aliases
jesse-engreitz:230531S019-THP1-P151-Amp_PPIF_enhancer-BioRep2-FFrep3-FACS_Input_AmpliconSequencing
Sequencing Results (Illumina)
8 items
Accession
File Format
Content Type
Illumina Read Type
Sequencing Run
Read Names
Associated seqspec Files
Sequencing Platform
Flowcell ID
Lane
Index
File Size
Lab
Upload Status
IGVFFI0896THZT
fastq
reads
I1
1
Illumina NextSeq 550
CAACTCTC:TTGT
960 KB
Jesse Engreitz, Stanford
validated
IGVFFI3649JAGF
fastq
reads
I1
1
Illumina NextSeq 550
AACGCATT:TTGT
928 KB
Jesse Engreitz, Stanford
validated
IGVFFI3805VYAH
fastq
reads
R2
1
Illumina NextSeq 550
CAACTCTC:TTGT
4.2 MB
Jesse Engreitz, Stanford
validated
IGVFFI4338UVFK
fastq
reads
R1
1
Illumina NextSeq 550
AACGCATT:TTGT
3.3 MB
Jesse Engreitz, Stanford
validated
IGVFFI4700NWVO
fastq
reads
I2
1
Illumina NextSeq 550
CAACTCTC:TTGT
959 KB
Jesse Engreitz, Stanford
validated
IGVFFI5505ATDV
fastq
reads
R1
1
Illumina NextSeq 550
CAACTCTC:TTGT
3.5 MB
Jesse Engreitz, Stanford
validated
IGVFFI6145UWTR
fastq
reads
I2
1
Illumina NextSeq 550
AACGCATT:TTGT
892 KB
Jesse Engreitz, Stanford
validated
IGVFFI9547HEZD
fastq
reads
R2
1
Illumina NextSeq 550
AACGCATT:TTGT
3.9 MB
Jesse Engreitz, Stanford
validated
Samples
1 item
Accession
Type
Sample Terms
Associated Construct Library Sets
Summary
IGVFSM5729BNQM
InVitroSystem
THP-1
IGVFDS2374NXLW
THP-1 cell line, male, Homo sapiens modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library
Donors
1 item
Accession
Aliases
Sex
Taxa
IGVFDO7359LVPI
igvf:donor_of_THP1
male
Homo sapiens
Associated Construct Library Sets
1 item
Accession
Summary
Library Design Files
IGVFDS2374NXLW
guide (sgRNA) library targeting sequence variants in a genomic locus
Control File Sets
1 item
Accession
Type
Summary
Aliases
Lab
IGVFDS4989HPTW
MeasurementSet
untransfected prime editing Variant-EFFECTS
jesse-engreitz:230531S019-THP1-P151-Amp_PPIF_enhancer-THP1-PE2_Exp1_NegControl_AmpliconSequencing
Jesse Engreitz, Stanford
File Sets Using This Measurement Set as an Input
1 item
Accession
Type
Summary
Aliases
Lab
IGVFDS5031MNRR
AnalysisSet
prime editing Variant-EFFECTS targeting PPIF: variant effects
jesse-engreitz:PPIF_enhancer_tiling_THP1_Variant-FlowFISH_analysis
Jesse Engreitz, Stanford
Attribution
Award
HG011972
Principal Investigator(s)
Anshul Kundaje
,
Jesse Engreitz
,
Marlene Rabinovitch
,
Lars Steinmetz
,
Michael Bassik
,
Thomas Quertermous
,
Will Greenleaf
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford