IGVFDS7957LQTQ

released
File Set Type
experimental data
Summary
cas mediated mutagenesis (Variant FlowFISH) integrating a guide (sgRNA) library targeting sequence variants in a genomic locus followed by DNA sequencing assay
Assay Term
Donors
Samples
Sample Summaries
  • THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 76-100% expression of PPIF) modified with a guide library
Construct Library Sets
  • IGVFDS2385DHZO Guide (sgRNA) library targeting sequence variants in a genomic locus
Aliases
jesse-engreitz:230523pPPIFEnformer-THP1-Amp_PPIF_promoter-BioRep2-FFrep4-BinD_AmpliconSequencing

Assay Details

Sequencing Results (Illumina)

48 items
fastq
reads
R2
1
Illumina NextSeq 550
AAGGATGT:TGAC
12 MB
Jesse Engreitz, Stanford
released
validated
fastq
reads
R1
1
Illumina NextSeq 550
AAGGATGT:TGAC
9.2 MB
Jesse Engreitz, Stanford
released
validated
fastq
reads
R2
1
Illumina NextSeq 550
TTGTCTAT:TGAC
12 MB
Jesse Engreitz, Stanford
released
validated
fastq
reads
I1
1
Illumina NextSeq 550
ATTCTAGG:TGAC
2.2 MB
Jesse Engreitz, Stanford
released
validated
fastq
reads
R2
1
Illumina NextSeq 550
TATCCAGG:TGAC
12 MB
Jesse Engreitz, Stanford
released
validated
fastq
reads
I1
1
Illumina NextSeq 550
TAATGAAC:TGAC
2.4 MB
Jesse Engreitz, Stanford
released
validated
fastq
reads
R1
1
Illumina NextSeq 550
TGCTGCTG:TGAC
9.1 MB
Jesse Engreitz, Stanford
released
validated
fastq
reads
R2
1
Illumina NextSeq 550
TAATGAAC:TGAC
12 MB
Jesse Engreitz, Stanford
released
validated
fastq
reads
I1
1
Illumina NextSeq 550
TACTTAGC:TGAC
2.1 MB
Jesse Engreitz, Stanford
released
validated
fastq
reads
I2
1
Illumina NextSeq 550
TGTAACTC:TGAC
27 KB
Jesse Engreitz, Stanford
released
validated

Control File Sets

1 item
Accession
Summary
Aliases
Lab
Status
cas mediated mutagenesis (Variant FlowFISH) followed by DNA sequencing assay
  • jesse-engreitz:230523pPPIFEnformer-THP1-Amp_PPIF_promoter-THP1-PE2_Exp4_NegControl_AmpliconSequencing
Jesse Engreitz, Stanford
released

Attribution