IGVFDS7957LQTQ

released
File Set Type
experimental data
Summary
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
Assay Term
Targeted Genes
Sample Summaries
  • THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 76-100% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library
Construct Library Sets
  • IGVFDS2385DHZO guide (sgRNA) library targeting sequence variants in a genomic locus
Protocols
Aliases
jesse-engreitz:230523pPPIFEnformer-THP1-Amp_PPIF_promoter-BioRep2-FFrep4-BinD_AmpliconSequencing

Sequencing Results (Illumina)

48 items
fastq
reads
R2
1
AAGGATGT:TGAC
12 MB
Jesse Engreitz, Stanford
validated
fastq
reads
R1
1
AAGGATGT:TGAC
9.2 MB
Jesse Engreitz, Stanford
validated
fastq
reads
R2
1
TTGTCTAT:TGAC
12 MB
Jesse Engreitz, Stanford
validated
fastq
reads
I1
1
ATTCTAGG:TGAC
2.2 MB
Jesse Engreitz, Stanford
validated
fastq
reads
R2
1
TATCCAGG:TGAC
12 MB
Jesse Engreitz, Stanford
validated
fastq
reads
I1
1
TAATGAAC:TGAC
2.4 MB
Jesse Engreitz, Stanford
validated
fastq
reads
R1
1
TGCTGCTG:TGAC
9.1 MB
Jesse Engreitz, Stanford
validated
fastq
reads
R2
1
TAATGAAC:TGAC
12 MB
Jesse Engreitz, Stanford
validated
fastq
reads
I1
1
TACTTAGC:TGAC
2.1 MB
Jesse Engreitz, Stanford
validated
fastq
reads
I2
1
TGTAACTC:TGAC
27 KB
Jesse Engreitz, Stanford
validated

Attribution