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IGVFDS7135ULJC

released
File Set Type
experimental data
Summary
mixed CRISPR TAP-seq integrating a guide (sgRNA) library targeting accessible genome regions in 29 genomic loci
Assay Term
in vitro CRISPR screen using single-cell RNA-seq
Sample Summaries
  • HCT116 cell line, male, Homo sapiens modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library
Construct Library Sets
  • IGVFDS9869EMNE guide (sgRNA) library targeting accessible genome regions in 29 genomic loci
Protocols
Aliases
jesse-engreitz:measurement_CRUDO_TAPseq-Auxin0hrs-Rep2

Sequencing Results (Illumina)

12 items
fastq
reads
R1
1
3
399 MB
Jesse Engreitz, Stanford
validated
fastq
reads
R2
1
3
898 MB
Jesse Engreitz, Stanford
validated
fastq
reads
R1
1
2
390 MB
Jesse Engreitz, Stanford
validated
fastq
reads
R2
1
2
875 MB
Jesse Engreitz, Stanford
validated
fastq
reads
R1
1
4
397 MB
Jesse Engreitz, Stanford
validated
fastq
reads
R2
1
1
883 MB
Jesse Engreitz, Stanford
validated
fastq
reads
R1
1
1
392 MB
Jesse Engreitz, Stanford
validated
fastq
reads
R2
1
4
890 MB
Jesse Engreitz, Stanford
validated
fastq
reads
I1
1
2
116 MB
Jesse Engreitz, Stanford
validated
fastq
reads
I1
1
1
116 MB
Jesse Engreitz, Stanford
validated

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
HCT116 cell line, male, Homo sapiens modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_HCT116
male
Homo sapiens

Auxiliary Datasets

1 item
Accession
Type
Summary
Files
Aliases
Lab
AuxiliarySet
gRNA sequencing for mixed CRISPR TAP-seq integrating a guide (sgRNA) library targeting accessible genome regions in 29 genomic loci
  • jesse-engreitz:auxiliary_CRUDO_TAPseq-Auxin0hrs-Rep2
Jesse Engreitz, Stanford

Documents

1 item
Page
Description
Type
Download
Preview
overview of experimental design
experimental protocol

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford