IGVFDS7135ULJC

released
{
    "@context": "/terms/",
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    "@type": [
        "MeasurementSet",
        "FileSet",
        "Item"
    ],
    "accession": "IGVFDS7135ULJC",
    "aliases": [
        "jesse-engreitz:measurement_CRUDO_TAPseq-Auxin0hrs-Rep2"
    ],
    "assay_term": {
        "@id": "/assay-terms/OBI_0003660/",
        "status": "released",
        "term_name": "in vitro CRISPR screen using single-cell RNA-seq"
    },
    "audit": {
        "INTERNAL_ACTION": [
            {
                "category": "missing sequencing kit",
                "detail": "Measurement set [IGVFDS7135ULJC](/measurement-sets/IGVFDS7135ULJC/) has sequence file(s) [IGVFFI7624ITNU](/sequence-files/IGVFFI7624ITNU/), [IGVFFI1923YHHK](/sequence-files/IGVFFI1923YHHK/), [IGVFFI8450TDKJ](/sequence-files/IGVFFI8450TDKJ/), [IGVFFI4471BAWM](/sequence-files/IGVFFI4471BAWM/), [IGVFFI7281TZZG](/sequence-files/IGVFFI7281TZZG/), [IGVFFI2893MYCT](/sequence-files/IGVFFI2893MYCT/), [IGVFFI4178KSHJ](/sequence-files/IGVFFI4178KSHJ/), [IGVFFI8305FKDJ](/sequence-files/IGVFFI8305FKDJ/), [IGVFFI5311ZUSI](/sequence-files/IGVFFI5311ZUSI/), [IGVFFI6429AOLS](/sequence-files/IGVFFI6429AOLS/), [IGVFFI7941GPIL](/sequence-files/IGVFFI7941GPIL/), [IGVFFI2780VBGU](/sequence-files/IGVFFI2780VBGU/) which lack specification of a `sequencing_kit`. Sequence files should specify a sequencing kit.",
                "level": 30,
                "level_name": "INTERNAL_ACTION",
                "name": "audit_inconsistent_sequencing_kit",
                "path": "/measurement-sets/IGVFDS7135ULJC/"
            },
            {
                "category": "missing sequencing kit",
                "detail": "Auxiliary set [IGVFDS8748OHPQ](/auxiliary-sets/IGVFDS8748OHPQ/) has sequence file(s) [IGVFFI2315CHAW](/sequence-files/IGVFFI2315CHAW/), [IGVFFI6591FOMG](/sequence-files/IGVFFI6591FOMG/), [IGVFFI1413KOZF](/sequence-files/IGVFFI1413KOZF/), [IGVFFI2373VGQQ](/sequence-files/IGVFFI2373VGQQ/), [IGVFFI5632DOTG](/sequence-files/IGVFFI5632DOTG/), [IGVFFI0448FLMO](/sequence-files/IGVFFI0448FLMO/), [IGVFFI5285IRWT](/sequence-files/IGVFFI5285IRWT/), [IGVFFI8825PYZU](/sequence-files/IGVFFI8825PYZU/), [IGVFFI6297ZONU](/sequence-files/IGVFFI6297ZONU/), [IGVFFI6523ECXR](/sequence-files/IGVFFI6523ECXR/), [IGVFFI7983EOQT](/sequence-files/IGVFFI7983EOQT/), [IGVFFI1717NNGG](/sequence-files/IGVFFI1717NNGG/) which lack specification of a `sequencing_kit`. Sequence files should specify a sequencing kit.",
                "level": 30,
                "level_name": "INTERNAL_ACTION",
                "name": "audit_inconsistent_sequencing_kit",
                "path": "/auxiliary-sets/IGVFDS8748OHPQ/"
            }
        ]
    },
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                "jesse-engreitz:auxiliary_CRUDO_TAPseq-Auxin0hrs-Rep2"
            ],
            "file_set_type": "gRNA sequencing",
            "status": "released"
        }
    ],
    "award": {
        "@id": "/awards/HG011972/",
        "component": "functional characterization",
        "contact_pi": {
            "@id": "/users/6c75dbfa-635e-4a7a-8ec8-9e51e9c380a4/",
            "title": "Jesse Engreitz"
        },
        "title": "Stanford Center for Connecting DNA Variants to Function and Phenotype"
    },
    "control_for": [],
    "creation_timestamp": "2024-05-23T22:48:13.532781+00:00",
    "documents": [
        "/documents/74d53b9c-ab1d-42e3-b679-d3a00ae3d842/"
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        {
            "@id": "/human-donors/IGVFDO0384OZRW/",
            "accession": "IGVFDO0384OZRW",
            "aliases": [
                "igvf:donor_of_HCT116"
            ],
            "sex": "male",
            "status": "released",
            "taxa": "Homo sapiens"
        }
    ],
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            "creation_timestamp": "2024-06-20T17:16:12.694452+00:00",
            "file_format": "yaml",
            "file_size": 1044,
            "href": "/configuration-files/IGVFFI9662WYOI/@@download/IGVFFI9662WYOI.yaml.gz",
            "s3_uri": "s3://igvf-files/2024/06/20/91edda77-6fbd-4118-9d97-0440e826e48b/IGVFFI9662WYOI.yaml.gz",
            "status": "released",
            "submitted_file_name": "/oak/stanford/groups/engreitz/Projects/Cohesin/240410_IGVF_upload/seqspec/tapseq_seqspec.out.Auxin0hrs-Rep2-S4.rna.yaml.gz",
            "upload_status": "validated"
        }
    ],
    "input_for": [
        "/analysis-sets/IGVFDS5308EMCG/"
    ],
    "lab": {
        "@id": "/labs/jesse-engreitz/",
        "title": "Jesse Engreitz, Stanford"
    },
    "notes": "The readout 86015c12-b9a6-43ba-9601-5d75aab4d39c was removed from this measurement set.",
    "preferred_assay_title": "TAP-seq",
    "protocols": [
        "https://www.protocols.io/view/crispri-of-regulatory-elements-upon-degron-operati-5jyl82d59l2w/v1"
    ],
    "release_timestamp": "2024-07-10T19:53:28.769965+00:00",
    "samples": [
        {
            "@id": "/in-vitro-systems/IGVFSM0767GJUV/",
            "@type": [
                "InVitroSystem",
                "Biosample",
                "Sample",
                "Item"
            ],
            "accession": "IGVFSM0767GJUV",
            "aliases": [
                "jesse-engreitz:in-vitro-system_CRUDO_TAPseq-Auxin0hrs-Rep2"
            ],
            "classifications": [
                "cell line"
            ],
            "construct_library_sets": [
                {
                    "@id": "/construct-library-sets/IGVFDS9869EMNE/",
                    "accession": "IGVFDS9869EMNE",
                    "file_set_type": "guide library",
                    "status": "released",
                    "summary": "guide (sgRNA) library targeting accessible genome regions in 29 genomic loci"
                }
            ],
            "modifications": [
                {
                    "@id": "/crispr-modifications/8543fa06-1720-4e7d-8e8f-7e24c1b1cf79/",
                    "modality": "interference",
                    "status": "released"
                },
                {
                    "@id": "/degron-modifications/2e8d10cc-fc98-44d5-a7d9-e2892e16c98a/",
                    "modality": "degradation",
                    "status": "released"
                }
            ],
            "sample_terms": [
                {
                    "@id": "/sample-terms/EFO_0002824/",
                    "@type": [
                        "SampleTerm",
                        "OntologyTerm",
                        "Item"
                    ],
                    "aliases": [
                        "igvf:HCT116"
                    ],
                    "status": "released",
                    "summary": "HCT116",
                    "term_name": "HCT116"
                }
            ],
            "status": "released",
            "summary": "HCT116 cell line, male, Homo sapiens modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library",
            "taxa": "Homo sapiens"
        }
    ],
    "schema_version": "23",
    "status": "released",
    "submitted_by": {
        "@id": "/users/3bd6f74c-8cf3-4c35-a94d-0c7c11654815/",
        "title": "Chongyon Park"
    },
    "submitted_files_timestamp": "2024-06-03T19:11:36.315221+00:00",
    "summary": "mixed CRISPR TAP-seq integrating a guide (sgRNA) library targeting accessible genome regions in 29 genomic loci",
    "uuid": "26e12241-ab13-4312-9d9c-aa8782baff35"
}