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IGVFDS6252SSYB

released
File Set Type
experimental data
Summary
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
Assay Term
in vitro CRISPR screen using flow cytometry
Targeted Genes
Sample Summaries
  • THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 26-50% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library
Construct Library Sets
  • IGVFDS2385DHZO guide (sgRNA) library targeting sequence variants in a genomic locus
Protocols
Aliases
jesse-engreitz:230523pPPIFEnformer-THP1-Amp_PPIF_promoter-BioRep2-FFrep3-BinB_AmpliconSequencing

Sequencing Results (Illumina)

48 items
fastq
reads
R2
1
CCAGCACC:TGAC
10 MB
Jesse Engreitz, Stanford
validated
fastq
reads
I2
1
CCAGCACC:TGAC
1.7 MB
Jesse Engreitz, Stanford
validated
fastq
reads
I2
1
AGGTTATC:TGAC
2.0 MB
Jesse Engreitz, Stanford
validated
fastq
reads
I1
1
GTCTGATG:TGAC
2.0 MB
Jesse Engreitz, Stanford
validated
fastq
reads
I1
1
AGGTTATC:TGAC
2.2 MB
Jesse Engreitz, Stanford
validated
fastq
reads
R2
1
CATGCTTA:TGAC
12 MB
Jesse Engreitz, Stanford
validated
fastq
reads
I2
1
AGGATCTA:TGAC
2.0 MB
Jesse Engreitz, Stanford
validated
fastq
reads
R1
1
CCAACATT:TGAC
9.0 MB
Jesse Engreitz, Stanford
validated
fastq
reads
R1
1
CAACTCTC:TGAC
9.1 MB
Jesse Engreitz, Stanford
validated
fastq
reads
R2
1
CTAACTCG:TGAC
11 MB
Jesse Engreitz, Stanford
validated

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford