IGVFDS5704ISFI

released
File Set Type
experimental data
Summary
cas mediated mutagenesis (Variant FlowFISH) integrating a guide (sgRNA) library targeting sequence variants in a genomic locus followed by DNA sequencing assay
Assay Term
Donors
Samples
Sample Summaries
  • Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 76-100% expression of PPIF) modified with a guide library
Construct Library Sets
  • IGVFDS2385DHZO Guide (sgRNA) library targeting sequence variants in a genomic locus
Aliases
jesse-engreitz:230423pPPIFEnformer-Jurkat-Amp_PPIF_promoter-BioRep1-FFrep1-BinD_AmpliconSequencing

Assay Details

Sequencing Results (Illumina)

48 items
fastq
reads
I1
1
Illumina NextSeq 550
TGTAATCA:CAGT
1.9 MB
Jesse Engreitz, Stanford
released
validated
fastq
reads
I1
1
Illumina NextSeq 550
TTAATCAG:CAGT
1.8 MB
Jesse Engreitz, Stanford
released
validated
fastq
reads
I2
1
Illumina NextSeq 550
CCTATGCC:CAGT
2.0 MB
Jesse Engreitz, Stanford
released
validated
fastq
reads
R2
1
Illumina NextSeq 550
CAGGAGCC:CAGT
9.5 MB
Jesse Engreitz, Stanford
released
validated
fastq
reads
I1
1
Illumina NextSeq 550
TGCAAGTA:CAGT
1.9 MB
Jesse Engreitz, Stanford
released
validated
fastq
reads
I2
1
Illumina NextSeq 550
TTCGCTGA:CAGT
2.0 MB
Jesse Engreitz, Stanford
released
validated
fastq
reads
R2
1
Illumina NextSeq 550
TAAGCACA:CAGT
10.0 MB
Jesse Engreitz, Stanford
released
validated
fastq
reads
I2
1
Illumina NextSeq 550
TTGAATAG:CAGT
2.1 MB
Jesse Engreitz, Stanford
released
validated
fastq
reads
I2
1
Illumina NextSeq 550
TGCAAGTA:CAGT
1.9 MB
Jesse Engreitz, Stanford
released
validated
fastq
reads
I1
1
Illumina NextSeq 550
TTCGCTGA:CAGT
1.9 MB
Jesse Engreitz, Stanford
released
validated

Control File Sets

1 item
Accession
Summary
Aliases
Lab
Status
cas mediated mutagenesis (Variant FlowFISH) followed by DNA sequencing assay
  • jesse-engreitz:230423pPPIFEnformer-Jurkat-Amp_PPIF_promoter-Jurkat-PE2_Exp2_NegControl_AmpliconSequencing
Jesse Engreitz, Stanford
released

Attribution