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INDEXED
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Measurement Set
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IGVFDS5704ISFI
IGVFDS5704ISFI
released
Json view
File Set Type
experimental data
Summary
cas mediated mutagenesis (Variant FlowFISH) integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
Assay Term
cas mediated mutagenesis
Sample Summaries
Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 76-100% expression of PPIF) modified with a guide library
Construct Library Sets
IGVFDS2385DHZO
Guide (sgRNA) library targeting sequence variants in a genomic locus
Protocols
https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1
Aliases
jesse-engreitz:230423pPPIFEnformer-Jurkat-Amp_PPIF_promoter-BioRep1-FFrep1-BinD_AmpliconSequencing
Samples
1 item
Accession
Type
Sample Terms
Summary
IGVFSM9226UZTE
InVitroSystem
Jurkat
Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 76-100% expression of PPIF) modified with a guide library
Donors
1 item
Accession
Aliases
Sex
Taxa
IGVFDO1080XFGV
igvf:donor_of_Jurkat
male
Homo sapiens
Sequencing Results (Illumina)
48 items
1
2
3
4
5
Accession
File Format
Content Type
Illumina Read Type
Sequencing Run
Associated seqspec Files
Sequencing Platform
Flowcell ID
Lane
Index
File Size
Lab
Upload Status
IGVFFI0171OZDZ
fastq
reads
I1
1
Illumina NextSeq 550
TGTAATCA:CAGT
1.9 MB
Jesse Engreitz, Stanford
validated
IGVFFI0197BRAF
fastq
reads
I1
1
Illumina NextSeq 550
TTAATCAG:CAGT
1.8 MB
Jesse Engreitz, Stanford
validated
IGVFFI0422PHBM
fastq
reads
I2
1
Illumina NextSeq 550
CCTATGCC:CAGT
2.0 MB
Jesse Engreitz, Stanford
validated
IGVFFI0848GDNO
fastq
reads
R2
1
Illumina NextSeq 550
CAGGAGCC:CAGT
9.5 MB
Jesse Engreitz, Stanford
validated
IGVFFI1014PBZO
fastq
reads
I1
1
Illumina NextSeq 550
TGCAAGTA:CAGT
1.9 MB
Jesse Engreitz, Stanford
validated
IGVFFI1505XVNP
fastq
reads
I2
1
Illumina NextSeq 550
TTCGCTGA:CAGT
2.0 MB
Jesse Engreitz, Stanford
validated
IGVFFI1883DKHL
fastq
reads
R2
1
Illumina NextSeq 550
TAAGCACA:CAGT
10.0 MB
Jesse Engreitz, Stanford
validated
IGVFFI2083YHGP
fastq
reads
I2
1
Illumina NextSeq 550
TTGAATAG:CAGT
2.1 MB
Jesse Engreitz, Stanford
validated
IGVFFI2147RQPO
fastq
reads
I2
1
Illumina NextSeq 550
TGCAAGTA:CAGT
1.9 MB
Jesse Engreitz, Stanford
validated
IGVFFI2399VDEC
fastq
reads
I1
1
Illumina NextSeq 550
TTCGCTGA:CAGT
1.9 MB
Jesse Engreitz, Stanford
validated
Control File Sets
1 item
Accession
Summary
Aliases
Lab
IGVFDS4869FXYY
cas mediated mutagenesis (Variant FlowFISH)
jesse-engreitz:230423pPPIFEnformer-Jurkat-Amp_PPIF_promoter-Jurkat-PE2_Exp2_NegControl_AmpliconSequencing
Jesse Engreitz, Stanford
Attribution
Award
HG011972
Principal Investigator(s)
Anshul Kundaje
,
Jesse Engreitz
,
Marlene Rabinovitch
,
Lars Steinmetz
,
Michael Bassik
,
Thomas Quertermous
,
Will Greenleaf
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford