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IGVFDS4825MNUM

released
File Set Type
experimental data
Summary
unsorted FACS input CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
Assay Term
in vitro CRISPR screen using flow cytometry
Sample Summaries
  • THP-1 cell line, male, Homo sapiens modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library
Construct Library Sets
  • IGVFDS2385DHZO guide (sgRNA) library targeting sequence variants in a genomic locus
Protocols
Aliases
jesse-engreitz:230523pPPIFEnformer-THP1-Amp_PPIF_promoter-BioRep2-FFrep4-FACS_Input_AmpliconSequencing

Sequencing Results (Illumina)

9 items
fastq
reads
R2
1
AGGATCTA:TTGT
11 MB
Jesse Engreitz, Stanford
validated
fastq
reads
R1
1
AGGATCTA:TTGT
9.0 MB
Jesse Engreitz, Stanford
validated
fastq
reads
R2
1
AACTTGAC:TGAC
11 MB
Jesse Engreitz, Stanford
validated
fastq
reads
I1
1
TCTGGCGA:TTGT
2.0 MB
Jesse Engreitz, Stanford
validated
fastq
reads
I2
1
AGGATCTA:TTGT
2.0 MB
Jesse Engreitz, Stanford
validated
fastq
reads
I2
1
TCTGGCGA:TTGT
2.1 MB
Jesse Engreitz, Stanford
validated
fastq
reads
I1
1
AGGATCTA:TTGT
2.1 MB
Jesse Engreitz, Stanford
validated
fastq
reads
R2
1
TCTGGCGA:TTGT
12 MB
Jesse Engreitz, Stanford
validated
fastq
reads
R1
1
TCTGGCGA:TTGT
9.3 MB
Jesse Engreitz, Stanford
validated

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford