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IGVFDS4745BLLN

released
File Set Type
experimental data
Control Type
unsorted FACS input
Summary
unsorted FACS input CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
Assay Term
in vitro CRISPR screen using flow cytometry
Sample Summaries
  • Homo sapiens (male) THP-1 cell line modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transduced (lentivirus) with a guide library
Protocols
Aliases
jesse-engreitz:230807S022-THP1-P153-Amp_PPIF_promoter-BioRep1-FFrep2-FACS_Input_AmpliconSequencing
Attribution
Jesse Engreitz, Stanford

Sequencing Results (Illumina)

16 items
Read Names
fastq
reads
R1
1
ACCAACTG:TTGT
4.7 MB
Jesse Engreitz, Stanford
validated
fastq
reads
I1
1
GTATAACA:TTGT
1.4 MB
Jesse Engreitz, Stanford
validated
fastq
reads
I2
1
TTCGCTGA:TTGT
1.3 MB
Jesse Engreitz, Stanford
validated
fastq
reads
I2
1
GGTCCAGA:TTGT
1.3 MB
Jesse Engreitz, Stanford
validated
fastq
reads
R1
1
TTCGCTGA:TTGT
4.5 MB
Jesse Engreitz, Stanford
validated
fastq
reads
I1
1
ACCAACTG:TTGT
1.3 MB
Jesse Engreitz, Stanford
validated
fastq
reads
I1
1
TTCGCTGA:TTGT
1.3 MB
Jesse Engreitz, Stanford
validated
fastq
reads
I1
1
GGTCCAGA:TTGT
1.3 MB
Jesse Engreitz, Stanford
validated
fastq
reads
I2
1
GTATAACA:TTGT
1.3 MB
Jesse Engreitz, Stanford
validated
fastq
reads
R2
1
TTCGCTGA:TTGT
5.6 MB
Jesse Engreitz, Stanford
validated

Samples

1 item
Accession
Type
Sample Terms
Associated Construct Library Sets
Summary
InVitroSystem
Homo sapiens (male) THP-1 cell line modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transduced (lentivirus) with a guide library

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_THP1
male
Homo sapiens

Associated Construct Library Sets

1 item
Accession
Summary
Library Design Files
guide (sgRNA) library targeting sequence variants in a genomic locus

Control File Sets

1 item
Accession
Type
Summary
Aliases
Lab
MeasurementSet
untransfected prime editing Variant-EFFECTS
  • jesse-engreitz:230807S022-THP1-P153-Amp_PPIF_promoter-THP1-PE2_Exp2_NegControl_AmpliconSequencing
Jesse Engreitz, Stanford

File Sets Using This Measurement Set as an Input

1 item
Accession
Type
Summary
Aliases
Lab
AnalysisSet
CRISPR prime editing Variant-EFFECTS targeting PPIF integrating a guide (sgRNA) library targeting sequence variants in a genomic locus with unsorted FACS input, untransfected controls
  • jesse-engreitz:PPIF_promoter_tiling_THP1_Variant-FlowFISH_analysis
Jesse Engreitz, Stanford