{
"@context": "/terms/",
"@id": "/measurement-sets/IGVFDS4745BLLN/",
"@type": [
"MeasurementSet",
"FileSet",
"Item"
],
"accession": "IGVFDS4745BLLN",
"aliases": [
"jesse-engreitz:230807S022-THP1-P153-Amp_PPIF_promoter-BioRep1-FFrep2-FACS_Input_AmpliconSequencing"
],
"assay_term": {
"@id": "/assay-terms/OBI_0003661/",
"assay_slims": [
"CRISPR screens"
],
"status": "released",
"term_name": "in vitro CRISPR screen using flow cytometry"
},
"audit": {
"INTERNAL_ACTION": [
{
"category": "mismatched status",
"detail": "Released in vitro system [IGVFSM5150FUTV](/in-vitro-systems/IGVFSM5150FUTV/) has in progress subobject construct library set [IGVFDS6982DESN](/construct-library-sets/IGVFDS6982DESN/).",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_item_status",
"path": "/in-vitro-systems/IGVFSM5150FUTV/"
},
{
"category": "missing publication",
"detail": "In vitro system [IGVFSM5150FUTV](/in-vitro-systems/IGVFSM5150FUTV/) has no `publications`. Released and archived samples are expected to be associated with a publication.",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_sample_missing_publication",
"path": "/in-vitro-systems/IGVFSM5150FUTV/"
},
{
"category": "missing publication",
"detail": "Measurement set [IGVFDS4745BLLN](/measurement-sets/IGVFDS4745BLLN/) has no `publications`. Released and archived file sets are expected to be associated with a publication.",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_file_set_missing_publication",
"path": "/measurement-sets/IGVFDS4745BLLN/"
},
{
"category": "missing sequencing kit",
"detail": "Measurement set [IGVFDS4745BLLN](/measurement-sets/IGVFDS4745BLLN/) has sequence file(s) [IGVFFI4532WDMM](/sequence-files/IGVFFI4532WDMM/), [IGVFFI1381KBIX](/sequence-files/IGVFFI1381KBIX/), [IGVFFI9480GLWL](/sequence-files/IGVFFI9480GLWL/), [IGVFFI2986OVGK](/sequence-files/IGVFFI2986OVGK/), [IGVFFI6465QWWS](/sequence-files/IGVFFI6465QWWS/), [IGVFFI4750UTWV](/sequence-files/IGVFFI4750UTWV/), [IGVFFI8666RYND](/sequence-files/IGVFFI8666RYND/), [IGVFFI8687NXCQ](/sequence-files/IGVFFI8687NXCQ/), [IGVFFI5098WNHY](/sequence-files/IGVFFI5098WNHY/), [IGVFFI5504EUGW](/sequence-files/IGVFFI5504EUGW/), [IGVFFI7805AHXC](/sequence-files/IGVFFI7805AHXC/), [IGVFFI7369RZPL](/sequence-files/IGVFFI7369RZPL/), [IGVFFI6647SHPJ](/sequence-files/IGVFFI6647SHPJ/), [IGVFFI7811XCYG](/sequence-files/IGVFFI7811XCYG/), [IGVFFI1981CGYC](/sequence-files/IGVFFI1981CGYC/), [IGVFFI9072RTVA](/sequence-files/IGVFFI9072RTVA/) which lack specification of a `sequencing_kit`. Sequence files in a file set associated wtih bulk data should specify a sequencing kit.",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_inconsistent_sequencing_kit",
"path": "/measurement-sets/IGVFDS4745BLLN/"
},
{
"category": "missing sequence specification",
"detail": "Measurement set [IGVFDS4745BLLN](/measurement-sets/IGVFDS4745BLLN/) has sequence file(s): [IGVFFI4532WDMM](/sequence-files/IGVFFI4532WDMM/), [IGVFFI1381KBIX](/sequence-files/IGVFFI1381KBIX/), [IGVFFI9480GLWL](/sequence-files/IGVFFI9480GLWL/), [IGVFFI2986OVGK](/sequence-files/IGVFFI2986OVGK/), [IGVFFI6465QWWS](/sequence-files/IGVFFI6465QWWS/), [IGVFFI4750UTWV](/sequence-files/IGVFFI4750UTWV/), [IGVFFI8666RYND](/sequence-files/IGVFFI8666RYND/), [IGVFFI8687NXCQ](/sequence-files/IGVFFI8687NXCQ/), [IGVFFI5098WNHY](/sequence-files/IGVFFI5098WNHY/), [IGVFFI5504EUGW](/sequence-files/IGVFFI5504EUGW/), [IGVFFI7805AHXC](/sequence-files/IGVFFI7805AHXC/), [IGVFFI7369RZPL](/sequence-files/IGVFFI7369RZPL/), [IGVFFI6647SHPJ](/sequence-files/IGVFFI6647SHPJ/), [IGVFFI7811XCYG](/sequence-files/IGVFFI7811XCYG/), [IGVFFI1981CGYC](/sequence-files/IGVFFI1981CGYC/), [IGVFFI9072RTVA](/sequence-files/IGVFFI9072RTVA/) which do not have any `seqspecs`. Sequence files in a file set associated with bulk data are expected to link to a sequence specification file.",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_missing_seqspec",
"path": "/measurement-sets/IGVFDS4745BLLN/"
}
],
"NOT_COMPLIANT": [
{
"category": "missing control for",
"detail": "Measurement set [IGVFDS4745BLLN](/measurement-sets/IGVFDS4745BLLN/) has no `control_for`. The `control_file_sets` should be patched on the file sets it serves as a control for and this property, `control_for`, will be reverse calculated. File sets that specify a control type are expected to be a control for other file sets.",
"level": 50,
"level_name": "NOT_COMPLIANT",
"name": "audit_control_for_control_type",
"path": "/measurement-sets/IGVFDS4745BLLN/"
}
],
"WARNING": [
{
"category": "missing auxiliary set",
"detail": "Measurement set [IGVFDS4745BLLN](/measurement-sets/IGVFDS4745BLLN/) has no cell sorting `auxiliary_sets`. CRISPR-based measurement sets that utilize flow cytometry are expected to link to a cell sorting auxiliary set.",
"level": 40,
"level_name": "WARNING",
"name": "audit_missing_auxiliary_set",
"path": "/measurement-sets/IGVFDS4745BLLN/"
},
{
"category": "missing targeted genes",
"detail": "Measurement set [IGVFDS4745BLLN](/measurement-sets/IGVFDS4745BLLN/) has no `targeted_genes`. ChIP-seq and CRISPR flow cytometry assays are expected to specify targeted gene(s).",
"level": 40,
"level_name": "WARNING",
"name": "audit_targeted_genes",
"path": "/measurement-sets/IGVFDS4745BLLN/"
}
]
},
"award": {
"@id": "/awards/HG011972/",
"component": "functional characterization",
"contact_pi": {
"@id": "/users/6c75dbfa-635e-4a7a-8ec8-9e51e9c380a4/",
"title": "Jesse Engreitz"
},
"title": "Stanford Center for Connecting DNA Variants to Function and Phenotype"
},
"construct_library_sets": [
{
"@id": "/construct-library-sets/IGVFDS6982DESN/",
"accession": "IGVFDS6982DESN",
"file_set_type": "guide library",
"status": "in progress",
"summary": "guide (sgRNA) library targeting sequence variants in a genomic locus"
}
],
"control_file_sets": [
{
"@id": "/measurement-sets/IGVFDS5864UGFT/",
"accession": "IGVFDS5864UGFT",
"aliases": [
"jesse-engreitz:230807S022-THP1-P153-Amp_PPIF_promoter-THP1-PE2_Exp2_NegControl_AmpliconSequencing"
],
"status": "released"
}
],
"control_for": [],
"control_type": "unsorted FACS input",
"creation_timestamp": "2023-11-30T22:49:36.885025+00:00",
"data_use_limitation_summaries": [
"no certificate"
],
"donors": [
{
"@id": "/human-donors/IGVFDO7359LVPI/",
"accession": "IGVFDO7359LVPI",
"aliases": [
"igvf:donor_of_THP1"
],
"sex": "male",
"status": "released",
"taxa": "Homo sapiens"
}
],
"externally_hosted": false,
"file_set_type": "experimental data",
"files": [
{
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"accession": "IGVFFI4532WDMM",
"aliases": [
"jesse-engreitz:230807S022-THP1-P153-Amp_PPIF_promoter-BioRep1-FFrep2-FACS_Input-PCRrep2_I2"
],
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"file_size": 1294902,
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"md5sum": "c7d584c3f6950494820972b1aded1c43",
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"sequencing_platform": {
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"status": "released",
"term_name": "Illumina NextSeq 550"
},
"status": "released",
"submitted_file_name": "230807S022-THP1-P153-Amp_PPIF_promoter-BioRep1-FFrep2-BinAll-PCRrep2_S395_I2_001.fastq.gz",
"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI1381KBIX/",
"accession": "IGVFFI1381KBIX",
"aliases": [
"jesse-engreitz:230807S022-THP1-P153-Amp_PPIF_promoter-BioRep1-FFrep2-FACS_Input-PCRrep1_R1"
],
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},
{
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},
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],
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},
{
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},
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},
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},
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