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INDEXED
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MeasurementSet
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IGVFDS4623JVYR
IGVFDS4623JVYR
released
Json view
File Set Type
experimental data
Control Type
unsorted FACS input
Summary
unsorted FACS input CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
Assay Term
in vitro CRISPR screen using flow cytometry
Sample Summaries
Jurkat cell line, male, Homo sapiens modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library
Protocols
https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1
Aliases
jesse-engreitz:230423pPPIFEnformer-Jurkat-Amp_PPIF_promoter-BioRep1-FFrep4-FACS_Input_AmpliconSequencing
Sequencing Results (Illumina)
8 items
Accession
File Format
Content Type
Illumina Read Type
Sequencing Run
Read Names
Associated seqspec Files
Sequencing Platform
Flowcell ID
Lane
Index
File Size
Lab
Upload Status
IGVFFI0108ECOI
fastq
reads
R2
1
Illumina NextSeq 550
ACAGGTAT:TTGT
9.0 MB
Jesse Engreitz, Stanford
validated
IGVFFI0777GNFL
fastq
reads
R1
1
Illumina NextSeq 550
ACAGGTAT:TTGT
6.6 MB
Jesse Engreitz, Stanford
validated
IGVFFI1144FSDA
fastq
reads
R2
1
Illumina NextSeq 550
AACGCATT:TTGT
9.3 MB
Jesse Engreitz, Stanford
validated
IGVFFI1282TRIL
fastq
reads
I1
1
Illumina NextSeq 550
ACAGGTAT:TTGT
2.0 MB
Jesse Engreitz, Stanford
validated
IGVFFI1417UIZK
fastq
reads
R1
1
Illumina NextSeq 550
AACGCATT:TTGT
6.8 MB
Jesse Engreitz, Stanford
validated
IGVFFI2225RQKE
fastq
reads
I2
1
Illumina NextSeq 550
ACAGGTAT:TTGT
1.8 MB
Jesse Engreitz, Stanford
validated
IGVFFI5117BYKA
fastq
reads
I1
1
Illumina NextSeq 550
AACGCATT:TTGT
2.0 MB
Jesse Engreitz, Stanford
validated
IGVFFI7853OOAF
fastq
reads
I2
1
Illumina NextSeq 550
AACGCATT:TTGT
1.9 MB
Jesse Engreitz, Stanford
validated
Samples
1 item
Accession
Type
Sample Terms
Associated Construct Library Sets
Summary
IGVFSM2087SKJC
InVitroSystem
Jurkat
IGVFDS2385DHZO
Jurkat cell line, male, Homo sapiens modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library
Donors
1 item
Accession
Aliases
Sex
Taxa
IGVFDO1080XFGV
igvf:donor_of_Jurkat
male
Homo sapiens
Associated Construct Library Sets
1 item
Accession
Summary
Library Design Files
IGVFDS2385DHZO
guide (sgRNA) library targeting sequence variants in a genomic locus
Control File Sets
1 item
Accession
Type
Summary
Aliases
Lab
IGVFDS4869FXYY
MeasurementSet
untransfected prime editing Variant-EFFECTS
jesse-engreitz:230423pPPIFEnformer-Jurkat-Amp_PPIF_promoter-Jurkat-PE2_Exp2_NegControl_AmpliconSequencing
Jesse Engreitz, Stanford
File Sets Using This Measurement Set as an Input
1 item
Accession
Type
Summary
Aliases
Lab
IGVFDS6299IJHG
AnalysisSet
prime editing Variant-EFFECTS targeting PPIF: variant effects
jesse-engreitz:PPIF_promoter_Enformer_edits_Jurkat_Variant-FlowFISH_analysis
Jesse Engreitz, Stanford
Attribution
Award
HG011972
Principal Investigator(s)
Anshul Kundaje
,
Jesse Engreitz
,
Marlene Rabinovitch
,
Lars Steinmetz
,
Michael Bassik
,
Thomas Quertermous
,
Will Greenleaf
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford