{
"@context": "/terms/",
"@id": "/measurement-sets/IGVFDS4623JVYR/",
"@type": [
"MeasurementSet",
"FileSet",
"Item"
],
"accession": "IGVFDS4623JVYR",
"aliases": [
"jesse-engreitz:230423pPPIFEnformer-Jurkat-Amp_PPIF_promoter-BioRep1-FFrep4-FACS_Input_AmpliconSequencing"
],
"assay_term": {
"@id": "/assay-terms/OBI_0003661/",
"status": "released",
"term_name": "in vitro CRISPR screen using flow cytometry"
},
"audit": {
"INTERNAL_ACTION": [
{
"category": "mismatched status",
"detail": "Released in vitro system [IGVFSM2087SKJC](/in-vitro-systems/IGVFSM2087SKJC/) has in progress subobject construct library set [IGVFDS2385DHZO](/construct-library-sets/IGVFDS2385DHZO/).",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_item_status",
"path": "/in-vitro-systems/IGVFSM2087SKJC/"
},
{
"category": "missing sequencing kit",
"detail": "Measurement set [IGVFDS4623JVYR](/measurement-sets/IGVFDS4623JVYR/) has sequence file(s) [IGVFFI0777GNFL](/sequence-files/IGVFFI0777GNFL/), [IGVFFI1282TRIL](/sequence-files/IGVFFI1282TRIL/), [IGVFFI5117BYKA](/sequence-files/IGVFFI5117BYKA/), [IGVFFI2225RQKE](/sequence-files/IGVFFI2225RQKE/), [IGVFFI7853OOAF](/sequence-files/IGVFFI7853OOAF/), [IGVFFI1417UIZK](/sequence-files/IGVFFI1417UIZK/), [IGVFFI0108ECOI](/sequence-files/IGVFFI0108ECOI/), [IGVFFI1144FSDA](/sequence-files/IGVFFI1144FSDA/) which lack specification of a `sequencing_kit`. Sequence files should specify a sequencing kit.",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_inconsistent_sequencing_kit",
"path": "/measurement-sets/IGVFDS4623JVYR/"
},
{
"category": "missing sequence specification",
"detail": "Measurement set [IGVFDS4623JVYR](/measurement-sets/IGVFDS4623JVYR/) has sequence file(s): [IGVFFI0777GNFL](/sequence-files/IGVFFI0777GNFL/), [IGVFFI1282TRIL](/sequence-files/IGVFFI1282TRIL/), [IGVFFI5117BYKA](/sequence-files/IGVFFI5117BYKA/), [IGVFFI2225RQKE](/sequence-files/IGVFFI2225RQKE/), [IGVFFI7853OOAF](/sequence-files/IGVFFI7853OOAF/), [IGVFFI1417UIZK](/sequence-files/IGVFFI1417UIZK/), [IGVFFI0108ECOI](/sequence-files/IGVFFI0108ECOI/), [IGVFFI1144FSDA](/sequence-files/IGVFFI1144FSDA/) which do not have any `seqspecs`. Sequence files in a file set are expected to link to a sequence specification file.",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_missing_seqspec",
"path": "/measurement-sets/IGVFDS4623JVYR/"
}
],
"WARNING": [
{
"category": "missing auxiliary set",
"detail": "Measurement set [IGVFDS4623JVYR](/measurement-sets/IGVFDS4623JVYR/) has no cell sorting `auxiliary_sets`. CRISPR-based measurement sets that utilize flow cytometry are expected to link to a cell sorting auxiliary set.",
"level": 40,
"level_name": "WARNING",
"name": "audit_missing_auxiliary_set",
"path": "/measurement-sets/IGVFDS4623JVYR/"
},
{
"category": "missing targeted genes",
"detail": "Measurement set [IGVFDS4623JVYR](/measurement-sets/IGVFDS4623JVYR/) has no `targeted_genes`. ChIP-seq and CRISPR flow cytometry assays are expected to specify targeted gene(s).",
"level": 40,
"level_name": "WARNING",
"name": "audit_targeted_genes",
"path": "/measurement-sets/IGVFDS4623JVYR/"
}
]
},
"award": {
"@id": "/awards/HG011972/",
"component": "functional characterization",
"contact_pi": {
"@id": "/users/6c75dbfa-635e-4a7a-8ec8-9e51e9c380a4/",
"title": "Jesse Engreitz"
},
"title": "Stanford Center for Connecting DNA Variants to Function and Phenotype"
},
"control_file_sets": [
{
"@id": "/measurement-sets/IGVFDS4869FXYY/",
"accession": "IGVFDS4869FXYY",
"aliases": [
"jesse-engreitz:230423pPPIFEnformer-Jurkat-Amp_PPIF_promoter-Jurkat-PE2_Exp2_NegControl_AmpliconSequencing"
],
"status": "released"
}
],
"control_for": [],
"control_type": "unsorted FACS input",
"creation_timestamp": "2023-12-13T08:48:09.565729+00:00",
"donors": [
{
"@id": "/human-donors/IGVFDO1080XFGV/",
"accession": "IGVFDO1080XFGV",
"aliases": [
"igvf:donor_of_Jurkat"
],
"sex": "male",
"status": "released",
"taxa": "Homo sapiens"
}
],
"externally_hosted": false,
"file_set_type": "experimental data",
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"accession": "IGVFFI0777GNFL",
"aliases": [
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"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2023-12-13T09:53:54.600919+00:00",
"file_format": "fastq",
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"sequencing_platform": {
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"status": "released",
"term_name": "Illumina NextSeq 550"
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"status": "released",
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"upload_status": "validated"
},
{
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"accession": "IGVFFI1282TRIL",
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"s3_uri": "s3://igvf-files/2023/12/13/807b1e41-2a41-41a1-8d8a-18c99cd9d772/IGVFFI1282TRIL.fastq.gz",
"sequencing_platform": {
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"status": "released",
"term_name": "Illumina NextSeq 550"
},
"status": "released",
"submitted_file_name": "230423pPPIFEnformerJurkat-Jurkat-PE-pPPIF_Enformer-Amp-pPPIF-BioRep1-FFRep4_BinAll-PCRrep2_S392_I1_001.fastq.gz",
"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI5117BYKA/",
"accession": "IGVFFI5117BYKA",
"aliases": [
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"creation_timestamp": "2023-12-13T15:26:43.483165+00:00",
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"file_size": 1960920,
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"status": "released",
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"status": "released",
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"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI2225RQKE/",
"accession": "IGVFFI2225RQKE",
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"controlled_access": false,
"creation_timestamp": "2023-12-13T16:30:19.325678+00:00",
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"sequencing_platform": {
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"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI7853OOAF/",
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"controlled_access": false,
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},
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"submitted_file_name": "230423pPPIFEnformerJurkat-Jurkat-PE-pPPIF_Enformer-Amp-pPPIF-BioRep1-FFRep4_BinAll-PCRrep1_S391_I2_001.fastq.gz",
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},
{
"@id": "/sequence-files/IGVFFI1417UIZK/",
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"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2023-12-13T09:53:07.343598+00:00",
"file_format": "fastq",
"file_size": 6755006,
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"status": "released",
"term_name": "Illumina NextSeq 550"
},
"status": "released",
"submitted_file_name": "230423pPPIFEnformerJurkat-Jurkat-PE-pPPIF_Enformer-Amp-pPPIF-BioRep1-FFRep4_BinAll-PCRrep1_S391_R1_001.fastq.gz",
"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI0108ECOI/",
"accession": "IGVFFI0108ECOI",
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"creation_timestamp": "2023-12-13T10:59:24.069584+00:00",
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},
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"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI1144FSDA/",
"accession": "IGVFFI1144FSDA",
"aliases": [
"jesse-engreitz:230423pPPIFEnformer-Jurkat-Amp_PPIF_promoter-BioRep1-FFrep4-FACS_Input-PCRrep1_R2"
],
"content_type": "reads",
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"creation_timestamp": "2023-12-13T10:58:34.968292+00:00",
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}
],
"input_for": [
"/analysis-sets/IGVFDS6299IJHG/"
],
"lab": {
"@id": "/labs/jesse-engreitz/",
"title": "Jesse Engreitz, Stanford"
},
"notes": "The readout 2e41e624-f6c7-4f0c-a07e-bc36cefdca39 was removed from this measurement set. This measurement set previously used Variant FlowFISH as a preferred_assay_title, but this enum is now removed. So it has been defaulted to Variant-EFFECTS.",
"preferred_assay_title": "Variant-EFFECTS",
"protocols": [
"https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1"
],
"release_timestamp": "2023-12-20T23:08:46.309836+00:00",
"samples": [
{
"@id": "/in-vitro-systems/IGVFSM2087SKJC/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM2087SKJC",
"aliases": [
"jesse-engreitz:230423pPPIFEnformer-Jurkat-Amp_PPIF_promoter-BioRep1-FFrep4-FACS_Input"
],
"classifications": [
"cell line"
],
"construct_library_sets": [
{
"@id": "/construct-library-sets/IGVFDS2385DHZO/",
"accession": "IGVFDS2385DHZO",
"file_set_type": "guide library",
"status": "in progress",
"summary": "guide (sgRNA) library targeting sequence variants in a genomic locus"
}
],
"modifications": [
{
"@id": "/crispr-modifications/fc6bc0b3-a064-47d0-b95d-a9af14bb1624/",
"modality": "prime editing",
"status": "released"
}
],
"sample_terms": [
{
"@id": "/sample-terms/EFO_0002796/",
"@type": [
"SampleTerm",
"OntologyTerm",
"Item"
],
"aliases": [
"igvf:Jurkat"
],
"status": "released",
"summary": "Jurkat",
"term_name": "Jurkat"
}
],
"status": "released",
"summary": "Jurkat cell line, male, Homo sapiens modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library",
"taxa": "Homo sapiens"
}
],
"schema_version": "23",
"status": "released",
"submitted_by": {
"@id": "/users/f9e7cd82-a407-4a20-89ee-f4dc668b0cbe/",
"title": "Michael Montgomery"
},
"submitted_files_timestamp": "2023-12-13T09:53:07.343598+00:00",
"summary": "unsorted FACS input CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus",
"uuid": "70450bf6-d4cc-4957-af0e-d175aaa7380d"
}