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IGVFDS4325LQED

released
File Set Type
experimental data
Summary
CRISPR knockout FACS screen sorted on the expression of IL7R integrating an expression vector library of BATF3 and a guide (sgRNA) library targeting transcription start sites in many genes
Assay Term
in vitro CRISPR screen using flow cytometry
Targeted Genes
Sample Summaries
  • Homo sapiens CD8-positive, alpha-beta memory T cell (sorting details: FACS bin for 90-100% expression of IL7R in cells overexpressing BATF3) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with multiple libraries (MOI of 0.4)
Protocols
Aliases
charles-gersbach:cd8-tcells-measurement-set-crispr-ko-tfome-batf3-over-high-bin-rep2
Publications
Attribution
Charles Gersbach, Duke

Sequencing Results (Illumina)

1 item
Accession
File Format
Content Type
Illumina Read Type
Sequencing Run
Read Names
Associated seqspec Files
Sequence Specification Document
Sequencing Platform
Flowcell ID
Lane
Index
File Size
Lab
Upload Status
fastq
reads
R1
2
AACJJ7LM5
1
697 MB
Charles Gersbach, Duke
validated

Samples

1 item
Accession
Type
Sample Terms
Associated Construct Library Sets
Summary
PrimaryCell
Homo sapiens CD8-positive, alpha-beta memory T cell (sorting details: FACS bin for 90-100% expression of IL7R in cells overexpressing BATF3) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with multiple libraries (MOI of 0.4)

Donors

1 item
Accession
Aliases
Sex
Taxa
  • charles-gersbach:cd8-tcells-donor-26
unspecified
Homo sapiens

Associated Construct Library Sets

2 items
Summary
Library Design Files
expression vector library of BATF3
guide (sgRNA) library targeting transcription start sites in many genes

Control File Sets

1 item
Accession
Type
Summary
Aliases
Lab
MeasurementSet
low FACS signal CRISPR knockout FACS screen sorted on the expression of IL7R integrating an expression vector library of BATF3 and a guide (sgRNA) library targeting transcription start sites in many genes
  • charles-gersbach:cd8-tcells-measurement-set-crispr-ko-tfome-batf3-over-low-bin-rep2
Charles Gersbach, Duke

File Sets Using This Measurement Set as an Input

1 item
Accession
Type
Summary
Aliases
Lab
AnalysisSet
CRISPR knockout FACS screen targeting IL7R integrating an expression vector library of BATF3 and a guide (sgRNA) library targeting transcription start sites in many genes with low FACS signal control
  • charles-gersbach:cd8-tcells-analysis-set-crispr-ko-tfome-batf3-over
Charles Gersbach, Duke