IGVFDS3485SAPD

released
File Set Type
experimental data
Summary
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
Assay Term
Targeted Genes
Sample Summaries
  • Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 76-100% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library
Construct Library Sets
  • IGVFDS2385DHZO guide (sgRNA) library targeting sequence variants in a genomic locus
Protocols
Aliases
jesse-engreitz:230423pPPIFEnformer-Jurkat-Amp_PPIF_promoter-BioRep1-FFrep4-BinD_AmpliconSequencing

Sequencing Results (Illumina)

48 items
fastq
reads
I1
1
TTATATCT:GTCA
2.4 MB
Jesse Engreitz, Stanford
validated
fastq
reads
R2
1
AAGGATGT:GTCA
13 MB
Jesse Engreitz, Stanford
validated
fastq
reads
I2
1
ATTCTAGG:GTCA
2.3 MB
Jesse Engreitz, Stanford
validated
fastq
reads
I1
1
TATCCAGG:GTCA
2.5 MB
Jesse Engreitz, Stanford
validated
fastq
reads
I1
1
CCAGAGCT:GTCA
2.6 MB
Jesse Engreitz, Stanford
validated
fastq
reads
I1
1
TACTTAGC:GTCA
2.6 MB
Jesse Engreitz, Stanford
validated
fastq
reads
I2
1
CCAGAGCT:GTCA
2.3 MB
Jesse Engreitz, Stanford
validated
fastq
reads
R2
1
TATCCAGG:GTCA
13 MB
Jesse Engreitz, Stanford
validated
fastq
reads
I2
1
TTGTCTAT:GTCA
2.3 MB
Jesse Engreitz, Stanford
validated
fastq
reads
R2
1
TAATGAAC:GTCA
13 MB
Jesse Engreitz, Stanford
validated

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 76-100% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_Jurkat
male
Homo sapiens

Control File Sets

1 item
Accession
Type
Summary
Aliases
Lab
MeasurementSet
untransfected prime editing Variant-EFFECTS
  • jesse-engreitz:230423pPPIFEnformer-Jurkat-Amp_PPIF_promoter-Jurkat-PE2_Exp2_NegControl_AmpliconSequencing
Jesse Engreitz, Stanford

File Sets Using This Measurement Set as an Input

1 item
Accession
Type
Summary
Aliases
Lab
AnalysisSet
prime editing Variant-EFFECTS targeting PPIF: variant effects
  • jesse-engreitz:PPIF_promoter_Enformer_edits_Jurkat_Variant-FlowFISH_analysis
Jesse Engreitz, Stanford

Attribution