IGVFDS3485SAPD

released
{
    "@context": "/terms/",
    "@id": "/measurement-sets/IGVFDS3485SAPD/",
    "@type": [
        "MeasurementSet",
        "FileSet",
        "Item"
    ],
    "accession": "IGVFDS3485SAPD",
    "aliases": [
        "jesse-engreitz:230423pPPIFEnformer-Jurkat-Amp_PPIF_promoter-BioRep1-FFrep4-BinD_AmpliconSequencing"
    ],
    "assay_term": {
        "@id": "/assay-terms/OBI_0003661/",
        "status": "released",
        "term_name": "in vitro CRISPR screen using flow cytometry"
    },
    "audit": {
        "INTERNAL_ACTION": [
            {
                "category": "mismatched status",
                "detail": "Released in vitro system [IGVFSM1740AUMH](/in-vitro-systems/IGVFSM1740AUMH/) has in progress subobject construct library set [IGVFDS2385DHZO](/construct-library-sets/IGVFDS2385DHZO/).",
                "level": 30,
                "level_name": "INTERNAL_ACTION",
                "name": "audit_item_status",
                "path": "/in-vitro-systems/IGVFSM1740AUMH/"
            },
            {
                "category": "missing sequencing kit",
                "detail": "Measurement set [IGVFDS3485SAPD](/measurement-sets/IGVFDS3485SAPD/) has sequence file(s) [IGVFFI2310WNUI](/sequence-files/IGVFFI2310WNUI/), [IGVFFI6868ZIZU](/sequence-files/IGVFFI6868ZIZU/), [IGVFFI2228PRWC](/sequence-files/IGVFFI2228PRWC/), [IGVFFI6599FITT](/sequence-files/IGVFFI6599FITT/), [IGVFFI3784NNIW](/sequence-files/IGVFFI3784NNIW/), [IGVFFI4966HGLS](/sequence-files/IGVFFI4966HGLS/), [IGVFFI4457HWQJ](/sequence-files/IGVFFI4457HWQJ/), [IGVFFI3737WHNM](/sequence-files/IGVFFI3737WHNM/), [IGVFFI7798HOHF](/sequence-files/IGVFFI7798HOHF/), [IGVFFI3553ISDU](/sequence-files/IGVFFI3553ISDU/), [IGVFFI2215YSMB](/sequence-files/IGVFFI2215YSMB/), [IGVFFI6588MUBD](/sequence-files/IGVFFI6588MUBD/), [IGVFFI6265AVEW](/sequence-files/IGVFFI6265AVEW/), [IGVFFI0953CGQB](/sequence-files/IGVFFI0953CGQB/), [IGVFFI5954LMRM](/sequence-files/IGVFFI5954LMRM/), [IGVFFI7509ZCDU](/sequence-files/IGVFFI7509ZCDU/), [IGVFFI1951QZWI](/sequence-files/IGVFFI1951QZWI/), [IGVFFI3834OCIC](/sequence-files/IGVFFI3834OCIC/), [IGVFFI8885BTAU](/sequence-files/IGVFFI8885BTAU/), [IGVFFI7342HLPV](/sequence-files/IGVFFI7342HLPV/), [IGVFFI1637MJBF](/sequence-files/IGVFFI1637MJBF/), [IGVFFI5094WSNI](/sequence-files/IGVFFI5094WSNI/), [IGVFFI5351WNRF](/sequence-files/IGVFFI5351WNRF/), [IGVFFI1721AWTM](/sequence-files/IGVFFI1721AWTM/), [IGVFFI8711JENQ](/sequence-files/IGVFFI8711JENQ/), [IGVFFI3402XYGE](/sequence-files/IGVFFI3402XYGE/), [IGVFFI9942BLFG](/sequence-files/IGVFFI9942BLFG/), [IGVFFI9553ZPOP](/sequence-files/IGVFFI9553ZPOP/), [IGVFFI9216PYYG](/sequence-files/IGVFFI9216PYYG/), [IGVFFI5267FJRZ](/sequence-files/IGVFFI5267FJRZ/), [IGVFFI2221GABI](/sequence-files/IGVFFI2221GABI/), [IGVFFI9635NZHA](/sequence-files/IGVFFI9635NZHA/), [IGVFFI4480TAYK](/sequence-files/IGVFFI4480TAYK/), [IGVFFI7332SJZD](/sequence-files/IGVFFI7332SJZD/), [IGVFFI3031LCGO](/sequence-files/IGVFFI3031LCGO/), [IGVFFI9743LUOW](/sequence-files/IGVFFI9743LUOW/), [IGVFFI4319GMWE](/sequence-files/IGVFFI4319GMWE/), [IGVFFI4811XJFY](/sequence-files/IGVFFI4811XJFY/), [IGVFFI6487WVVT](/sequence-files/IGVFFI6487WVVT/), [IGVFFI7133FKPH](/sequence-files/IGVFFI7133FKPH/), [IGVFFI6598PFOW](/sequence-files/IGVFFI6598PFOW/), [IGVFFI0369XBXG](/sequence-files/IGVFFI0369XBXG/), [IGVFFI0886PBVO](/sequence-files/IGVFFI0886PBVO/), [IGVFFI1105ARQQ](/sequence-files/IGVFFI1105ARQQ/), [IGVFFI3957ZXFV](/sequence-files/IGVFFI3957ZXFV/), [IGVFFI3129KVKC](/sequence-files/IGVFFI3129KVKC/), [IGVFFI4183BLYM](/sequence-files/IGVFFI4183BLYM/), [IGVFFI0905GRJE](/sequence-files/IGVFFI0905GRJE/) which lack specification of a `sequencing_kit`. Sequence files should specify a sequencing kit.",
                "level": 30,
                "level_name": "INTERNAL_ACTION",
                "name": "audit_inconsistent_sequencing_kit",
                "path": "/measurement-sets/IGVFDS3485SAPD/"
            },
            {
                "category": "missing sequence specification",
                "detail": "Measurement set [IGVFDS3485SAPD](/measurement-sets/IGVFDS3485SAPD/) has sequence file(s): [IGVFFI2310WNUI](/sequence-files/IGVFFI2310WNUI/), [IGVFFI6868ZIZU](/sequence-files/IGVFFI6868ZIZU/), [IGVFFI2228PRWC](/sequence-files/IGVFFI2228PRWC/), [IGVFFI6599FITT](/sequence-files/IGVFFI6599FITT/), [IGVFFI3784NNIW](/sequence-files/IGVFFI3784NNIW/), [IGVFFI4966HGLS](/sequence-files/IGVFFI4966HGLS/), [IGVFFI4457HWQJ](/sequence-files/IGVFFI4457HWQJ/), [IGVFFI3737WHNM](/sequence-files/IGVFFI3737WHNM/), [IGVFFI7798HOHF](/sequence-files/IGVFFI7798HOHF/), [IGVFFI3553ISDU](/sequence-files/IGVFFI3553ISDU/), [IGVFFI2215YSMB](/sequence-files/IGVFFI2215YSMB/), [IGVFFI6588MUBD](/sequence-files/IGVFFI6588MUBD/), [IGVFFI6265AVEW](/sequence-files/IGVFFI6265AVEW/), [IGVFFI0953CGQB](/sequence-files/IGVFFI0953CGQB/), [IGVFFI5954LMRM](/sequence-files/IGVFFI5954LMRM/), [IGVFFI7509ZCDU](/sequence-files/IGVFFI7509ZCDU/), [IGVFFI1951QZWI](/sequence-files/IGVFFI1951QZWI/), [IGVFFI3834OCIC](/sequence-files/IGVFFI3834OCIC/), [IGVFFI8885BTAU](/sequence-files/IGVFFI8885BTAU/), [IGVFFI7342HLPV](/sequence-files/IGVFFI7342HLPV/), [IGVFFI1637MJBF](/sequence-files/IGVFFI1637MJBF/), [IGVFFI5094WSNI](/sequence-files/IGVFFI5094WSNI/), [IGVFFI5351WNRF](/sequence-files/IGVFFI5351WNRF/), [IGVFFI1721AWTM](/sequence-files/IGVFFI1721AWTM/), [IGVFFI8711JENQ](/sequence-files/IGVFFI8711JENQ/), [IGVFFI3402XYGE](/sequence-files/IGVFFI3402XYGE/), [IGVFFI9942BLFG](/sequence-files/IGVFFI9942BLFG/), [IGVFFI9553ZPOP](/sequence-files/IGVFFI9553ZPOP/), [IGVFFI9216PYYG](/sequence-files/IGVFFI9216PYYG/), [IGVFFI5267FJRZ](/sequence-files/IGVFFI5267FJRZ/), [IGVFFI2221GABI](/sequence-files/IGVFFI2221GABI/), [IGVFFI9635NZHA](/sequence-files/IGVFFI9635NZHA/), [IGVFFI4480TAYK](/sequence-files/IGVFFI4480TAYK/), [IGVFFI7332SJZD](/sequence-files/IGVFFI7332SJZD/), [IGVFFI3031LCGO](/sequence-files/IGVFFI3031LCGO/), [IGVFFI9743LUOW](/sequence-files/IGVFFI9743LUOW/), [IGVFFI4319GMWE](/sequence-files/IGVFFI4319GMWE/), [IGVFFI4811XJFY](/sequence-files/IGVFFI4811XJFY/), [IGVFFI6487WVVT](/sequence-files/IGVFFI6487WVVT/), [IGVFFI7133FKPH](/sequence-files/IGVFFI7133FKPH/), [IGVFFI6598PFOW](/sequence-files/IGVFFI6598PFOW/), [IGVFFI0369XBXG](/sequence-files/IGVFFI0369XBXG/), [IGVFFI0886PBVO](/sequence-files/IGVFFI0886PBVO/), [IGVFFI1105ARQQ](/sequence-files/IGVFFI1105ARQQ/), [IGVFFI3957ZXFV](/sequence-files/IGVFFI3957ZXFV/), [IGVFFI3129KVKC](/sequence-files/IGVFFI3129KVKC/), [IGVFFI4183BLYM](/sequence-files/IGVFFI4183BLYM/), [IGVFFI0905GRJE](/sequence-files/IGVFFI0905GRJE/) which do not have any `seqspecs`. Sequence files in a file set are expected to link to a sequence specification file.",
                "level": 30,
                "level_name": "INTERNAL_ACTION",
                "name": "audit_missing_seqspec",
                "path": "/measurement-sets/IGVFDS3485SAPD/"
            }
        ],
        "WARNING": [
            {
                "category": "missing auxiliary set",
                "detail": "Measurement set [IGVFDS3485SAPD](/measurement-sets/IGVFDS3485SAPD/) has no cell sorting `auxiliary_sets`. CRISPR-based measurement sets that utilize flow cytometry are expected to link to a cell sorting auxiliary set.",
                "level": 40,
                "level_name": "WARNING",
                "name": "audit_missing_auxiliary_set",
                "path": "/measurement-sets/IGVFDS3485SAPD/"
            }
        ]
    },
    "award": {
        "@id": "/awards/HG011972/",
        "component": "functional characterization",
        "contact_pi": {
            "@id": "/users/6c75dbfa-635e-4a7a-8ec8-9e51e9c380a4/",
            "title": "Jesse Engreitz"
        },
        "title": "Stanford Center for Connecting DNA Variants to Function and Phenotype"
    },
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            "@id": "/measurement-sets/IGVFDS4869FXYY/",
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                "jesse-engreitz:230423pPPIFEnformer-Jurkat-Amp_PPIF_promoter-Jurkat-PE2_Exp2_NegControl_AmpliconSequencing"
            ],
            "status": "released"
        }
    ],
    "control_for": [],
    "creation_timestamp": "2023-12-13T08:48:09.216948+00:00",
    "donors": [
        {
            "@id": "/human-donors/IGVFDO1080XFGV/",
            "accession": "IGVFDO1080XFGV",
            "aliases": [
                "igvf:donor_of_Jurkat"
            ],
            "sex": "male",
            "status": "released",
            "taxa": "Homo sapiens"
        }
    ],
    "externally_hosted": false,
    "file_set_type": "experimental data",
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        },
        {
            "@id": "/sequence-files/IGVFFI2215YSMB/",
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            "s3_uri": "s3://igvf-files/2023/12/13/c87ce2b3-82dd-412c-9f07-4baadf086941/IGVFFI0905GRJE.fastq.gz",
            "sequencing_platform": {
                "@id": "/platform-terms/EFO_0008566/",
                "status": "released",
                "term_name": "Illumina NextSeq 550"
            },
            "status": "released",
            "submitted_file_name": "230423pPPIFEnformerJurkat-Jurkat-PE-pPPIF_Enformer-Amp-pPPIF-BioRep1-FFRep4_BinD-PCRrep4_S184_I2_001.fastq.gz",
            "upload_status": "validated"
        }
    ],
    "input_for": [
        "/analysis-sets/IGVFDS6299IJHG/"
    ],
    "lab": {
        "@id": "/labs/jesse-engreitz/",
        "title": "Jesse Engreitz, Stanford"
    },
    "notes": "The readout 2e41e624-f6c7-4f0c-a07e-bc36cefdca39 was removed from this measurement set. This measurement set previously used Variant FlowFISH as a preferred_assay_title, but this enum is now removed. So it has been defaulted to Variant-EFFECTS.",
    "preferred_assay_title": "Variant-EFFECTS",
    "protocols": [
        "https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1"
    ],
    "release_timestamp": "2023-12-20T23:08:24.328822+00:00",
    "samples": [
        {
            "@id": "/in-vitro-systems/IGVFSM1740AUMH/",
            "@type": [
                "InVitroSystem",
                "Biosample",
                "Sample",
                "Item"
            ],
            "accession": "IGVFSM1740AUMH",
            "aliases": [
                "jesse-engreitz:230423pPPIFEnformer-Jurkat-Amp_PPIF_promoter-BioRep1-FFrep4-BinD"
            ],
            "classifications": [
                "cell line"
            ],
            "construct_library_sets": [
                {
                    "@id": "/construct-library-sets/IGVFDS2385DHZO/",
                    "accession": "IGVFDS2385DHZO",
                    "file_set_type": "guide library",
                    "status": "in progress",
                    "summary": "guide (sgRNA) library targeting sequence variants in a genomic locus"
                }
            ],
            "modifications": [
                {
                    "@id": "/crispr-modifications/fc6bc0b3-a064-47d0-b95d-a9af14bb1624/",
                    "modality": "prime editing",
                    "status": "released"
                }
            ],
            "sample_terms": [
                {
                    "@id": "/sample-terms/EFO_0002796/",
                    "@type": [
                        "SampleTerm",
                        "OntologyTerm",
                        "Item"
                    ],
                    "aliases": [
                        "igvf:Jurkat"
                    ],
                    "status": "released",
                    "summary": "Jurkat",
                    "term_name": "Jurkat"
                }
            ],
            "status": "released",
            "summary": "Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 76-100% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library",
            "taxa": "Homo sapiens"
        }
    ],
    "schema_version": "23",
    "status": "released",
    "submitted_by": {
        "@id": "/users/f9e7cd82-a407-4a20-89ee-f4dc668b0cbe/",
        "title": "Michael Montgomery"
    },
    "submitted_files_timestamp": "2023-12-13T09:52:57.879066+00:00",
    "summary": "CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus",
    "targeted_genes": [
        {
            "@id": "/genes/ENSG00000108179/",
            "geneid": "ENSG00000108179",
            "name": "peptidylprolyl isomerase F",
            "status": "released",
            "symbol": "PPIF",
            "synonyms": [
                "rotamase F",
                "CYP3",
                "cyclophilin F",
                "mitochondrial cyclophilin",
                "peptidyl-prolyl cis-trans isomerase, mitochondrial",
                "CypD",
                "Cyp-D",
                "cyclophilin 3",
                "cyclophilin D",
                "CyP-M",
                "peptidyl-prolyl cis-trans isomerase F, mitochondrial",
                "PPIase F"
            ]
        }
    ],
    "uuid": "6aae81e3-c663-4244-a099-4d23cfbc263e"
}