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IGVFDS3458HJKJ

released
File Set Type
experimental data
Summary
CRISPR interference FlowFISH screen sorted on the expression of NFKBIA integrating a guide (sgRNA) library targeting phenotype-associated variants genome-wide associated with blood pressure
Assay Term
in vitro CRISPR screen using flow cytometry
Targeted Genes
Sample Summaries
  • TeloHAEC cell line, female, Homo sapiens (sorting details: FACS 20-40% expression of NFKBIA) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library
Protocols
Aliases
guillaume-lettre:BP_screen_KRAB_L_R2_2_ms

Sequencing Results (Illumina)

2 items
Read Names
fastq
reads
R2
1
2.6 GB
Guillaume Lettre, MHI
validated
fastq
reads
R1
1
1.8 GB
Guillaume Lettre, MHI
validated

Samples

1 item
Accession
Type
Sample Terms
Associated Construct Library Sets
Summary
InVitroSystem
TeloHAEC cell line, female, Homo sapiens (sorting details: FACS 20-40% expression of NFKBIA) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_teloHAEC
female
Homo sapiens

Associated Construct Library Sets

2 items
Summary
Library Design Files
guide (sgRNA) library targeting phenotype-associated variants genome-wide associated with blood pressure
guide (sgRNA) library targeting phenotype-associated variants genome-wide associated with blood pressure

File Sets Using This Measurement Set as an Input

1 item
Accession
Type
Summary
Aliases
Lab
AnalysisSet
CRISPR interference FlowFISH screen, Proliferation CRISPR interference screen targeting NFKBIA: guide quantifications
  • guillaume-lettre:BP_KRAB_flowfish_analysis_set
Guillaume Lettre, MHI

Attribution

Award
HG012010
Principal Investigator(s)
Contact P.I.
Luca Pinello
Lab
Guillaume Lettre, MHI