IGVFDS2690TJJM

released
File Set Type
experimental data
Summary
cas mediated mutagenesis (Variant FlowFISH) integrating a guide (sgRNA) library targeting sequence variants in a genomic locus followed by DNA sequencing assay
Assay Term
Donors
Samples
Sample Summaries
  • THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 76-100% expression of PPIF) modified with a guide library
Construct Library Sets
  • IGVFDS2374NXLW Guide (sgRNA) library targeting sequence variants in a genomic locus
Aliases
jesse-engreitz:230531S019-THP1-P151-Amp_PPIF_enhancer-BioRep2-FFrep4-BinD_AmpliconSequencing

Assay Details

Sequencing Results (Illumina)

48 items
fastq
reads
R2
1
Illumina NextSeq 550
TGTAACTC:ACTG
3.9 MB
Jesse Engreitz, Stanford
released
validated
fastq
reads
R1
1
Illumina NextSeq 550
TGTAACTC:TGAC
3.0 MB
Jesse Engreitz, Stanford
released
validated
fastq
reads
I2
1
Illumina NextSeq 550
TGCTGCTG:ACTG
887 KB
Jesse Engreitz, Stanford
released
validated
fastq
reads
I1
1
Illumina NextSeq 550
TGTAACTC:ACTG
893 KB
Jesse Engreitz, Stanford
released
validated
fastq
reads
R1
1
Illumina NextSeq 550
TATCCAGG:TGAC
3.0 MB
Jesse Engreitz, Stanford
released
validated
fastq
reads
R1
1
Illumina NextSeq 550
TTGTCTAT:TGAC
3.0 MB
Jesse Engreitz, Stanford
released
validated
fastq
reads
R1
1
Illumina NextSeq 550
TTGTCTAT:ACTG
3.2 MB
Jesse Engreitz, Stanford
released
validated
fastq
reads
I1
1
Illumina NextSeq 550
TGCTGCTG:ACTG
844 KB
Jesse Engreitz, Stanford
released
validated
fastq
reads
I1
1
Illumina NextSeq 550
TGCTGCTG:TGAC
808 KB
Jesse Engreitz, Stanford
released
validated
fastq
reads
I2
1
Illumina NextSeq 550
TTGTCTAT:ACTG
853 KB
Jesse Engreitz, Stanford
released
validated

Control File Sets

1 item
Accession
Summary
Aliases
Lab
Status
cas mediated mutagenesis (Variant FlowFISH) followed by DNA sequencing assay
  • jesse-engreitz:230531S019-THP1-P151-Amp_PPIF_enhancer-THP1-PE2_Exp1_NegControl_AmpliconSequencing
Jesse Engreitz, Stanford
released

Attribution