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INDEXED
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MeasurementSet
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IGVFDS2294QFQV
IGVFDS2294QFQV
released
Json view
File Set Type
experimental data
Summary
unsorted FACS input CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
Assay Term
in vitro CRISPR screen using flow cytometry
Sample Summaries
THP-1 cell line, male, Homo sapiens modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library
Construct Library Sets
IGVFDS2385DHZO
guide (sgRNA) library targeting sequence variants in a genomic locus
Protocols
https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1
Aliases
jesse-engreitz:230523pPPIFEnformer-THP1-Amp_PPIF_promoter-BioRep2-FFrep3-FACS_Input_AmpliconSequencing
Sequencing Results (Illumina)
8 items
Accession
File Format
Content Type
Illumina Read Type
Sequencing Run
Associated seqspec Files
Sequencing Platform
Flowcell ID
Lane
Index
File Size
Lab
Upload Status
IGVFFI1568WAJV
fastq
reads
R2
1
Illumina NextSeq 550
CATGCTTA:TTGT
12 MB
Jesse Engreitz, Stanford
validated
IGVFFI2442QEWF
fastq
reads
I1
1
Illumina NextSeq 550
CATGCTTA:TTGT
2.2 MB
Jesse Engreitz, Stanford
validated
IGVFFI2671WOEH
fastq
reads
I1
1
Illumina NextSeq 550
GGTCCAGA:TTGT
2.1 MB
Jesse Engreitz, Stanford
validated
IGVFFI3319FOYH
fastq
reads
R2
1
Illumina NextSeq 550
GGTCCAGA:TTGT
12 MB
Jesse Engreitz, Stanford
validated
IGVFFI3911TJMQ
fastq
reads
R1
1
Illumina NextSeq 550
CATGCTTA:TTGT
9.6 MB
Jesse Engreitz, Stanford
validated
IGVFFI8823EBJO
fastq
reads
R1
1
Illumina NextSeq 550
GGTCCAGA:TTGT
9.5 MB
Jesse Engreitz, Stanford
validated
IGVFFI9805KWPC
fastq
reads
I2
1
Illumina NextSeq 550
CATGCTTA:TTGT
2.1 MB
Jesse Engreitz, Stanford
validated
IGVFFI9963VRYP
fastq
reads
I2
1
Illumina NextSeq 550
GGTCCAGA:TTGT
2.1 MB
Jesse Engreitz, Stanford
validated
Samples
1 item
Accession
Type
Sample Terms
Summary
IGVFSM6859MCDG
InVitroSystem
THP-1
THP-1 cell line, male, Homo sapiens modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library
Donors
1 item
Accession
Aliases
Sex
Taxa
IGVFDO7359LVPI
igvf:donor_of_THP1
male
Homo sapiens
Control File Sets
1 item
Accession
Type
Summary
Aliases
Lab
IGVFDS5128CXIS
MeasurementSet
untransfected prime editing Variant-EFFECTS
jesse-engreitz:230523pPPIFEnformer-THP1-Amp_PPIF_promoter-THP1-PE2_Exp4_NegControl_AmpliconSequencing
Jesse Engreitz, Stanford
File Sets Using This Measurement Set as an Input
1 item
Accession
Type
Summary
Aliases
Lab
IGVFDS4359OODY
AnalysisSet
prime editing Variant-EFFECTS targeting PPIF: variant effects
jesse-engreitz:PPIF_promoter_Enformer_edits_THP1_Variant-FlowFISH_analysis
Jesse Engreitz, Stanford
Attribution
Award
HG011972
Principal Investigator(s)
Anshul Kundaje
,
Jesse Engreitz
,
Marlene Rabinovitch
,
Lars Steinmetz
,
Michael Bassik
,
Thomas Quertermous
,
Will Greenleaf
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford