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IGVFDS1434CFNM

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File Set Type
experimental data
Summary
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
Assay Term
in vitro CRISPR screen using flow cytometry
Targeted Genes
Sample Summaries
  • Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 76-100% expression of IL2RA) treated with 100 ng/mL phorbol 13-acetate 12-myristate for 24 hours, 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 24 hours, modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library
Construct Library Sets
  • IGVFDS6128KVUP guide (sgRNA) library targeting sequence variants in a genomic locus
Protocols
Aliases
jesse-engreitz:Jurkat_Stim-IL2RA_prom-BioRep1-FFRep2-BinD_AmpliconSequencing

Sequencing Results (Illumina)

32 items
fastq
reads
I1
1
GCCGTCGA:TTGT
1.6 MB
Jesse Engreitz, Stanford
validated
fastq
reads
R2
1
GCCTAGCC:TTGT
4.9 MB
Jesse Engreitz, Stanford
validated
fastq
reads
I2
1
ACTAAGAC:TTGT
3.2 MB
Jesse Engreitz, Stanford
validated
fastq
reads
I2
1
GCCGTCGA:GGCA
1.9 MB
Jesse Engreitz, Stanford
validated
fastq
reads
I2
1
GCCGTCGA:TTGT
1.5 MB
Jesse Engreitz, Stanford
validated
fastq
reads
R2
1
ACTAAGAC:GGCA
8.3 MB
Jesse Engreitz, Stanford
validated
fastq
reads
R2
1
ACAGGTAT:TTGT
5.9 MB
Jesse Engreitz, Stanford
validated
fastq
reads
R1
1
GCCGTCGA:TTGT
5.7 MB
Jesse Engreitz, Stanford
validated
fastq
reads
R2
1
GCCGTCGA:TTGT
6.2 MB
Jesse Engreitz, Stanford
validated
fastq
reads
I1
1
ACAGGTAT:TTGT
1.6 MB
Jesse Engreitz, Stanford
validated

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 76-100% expression of IL2RA) treated with 100 ng/mL phorbol 13-acetate 12-myristate for 24 hours, 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 24 hours, modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_Jurkat
male
Homo sapiens

Control File Sets

1 item
Accession
Type
Summary
Aliases
Lab
MeasurementSet
prime editing Variant-EFFECTS
  • jesse-engreitz:Jurkat_Stim-IL2RA_prom-NegControl_AmpliconSequencing
Jesse Engreitz, Stanford

File Sets Using This Measurement Set as an Input

1 item
Accession
Type
Summary
Aliases
Lab
AnalysisSet
prime editing Variant-EFFECTS targeting IL2RA: variant effects
  • jesse-engreitz:IL2RA_promoter_tiling_Jurkat-Stimulation_Variant-EFFECTS_analysis
Jesse Engreitz, Stanford

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford