{
"@context": "/terms/",
"@id": "/measurement-sets/IGVFDS1434CFNM/",
"@type": [
"MeasurementSet",
"FileSet",
"Item"
],
"accession": "IGVFDS1434CFNM",
"aliases": [
"jesse-engreitz:Jurkat_Stim-IL2RA_prom-BioRep1-FFRep2-BinD_AmpliconSequencing"
],
"assay_term": {
"@id": "/assay-terms/OBI_0003661/",
"status": "released",
"term_name": "in vitro CRISPR screen using flow cytometry"
},
"audit": {
"INTERNAL_ACTION": [
{
"category": "mismatched status",
"detail": "Preview in vitro system [IGVFSM9448WLVQ](/in-vitro-systems/IGVFSM9448WLVQ/) has in progress subobject treatment [cf87f410-54ee-48ef-b654-8443c4fee032](/treatments/cf87f410-54ee-48ef-b654-8443c4fee032/).",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_item_status",
"path": "/in-vitro-systems/IGVFSM9448WLVQ/"
},
{
"category": "mismatched status",
"detail": "Preview in vitro system [IGVFSM9448WLVQ](/in-vitro-systems/IGVFSM9448WLVQ/) has in progress subobject treatment [4b5f0d13-ec20-4b91-85f5-a009cd91451f](/treatments/4b5f0d13-ec20-4b91-85f5-a009cd91451f/).",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_item_status",
"path": "/in-vitro-systems/IGVFSM9448WLVQ/"
},
{
"category": "missing sequencing kit",
"detail": "Measurement set [IGVFDS1434CFNM](/measurement-sets/IGVFDS1434CFNM/) has sequence file(s) [IGVFFI3628PTRU](/sequence-files/IGVFFI3628PTRU/), [IGVFFI8190LTYK](/sequence-files/IGVFFI8190LTYK/), [IGVFFI8296ORHC](/sequence-files/IGVFFI8296ORHC/), [IGVFFI1885EORP](/sequence-files/IGVFFI1885EORP/), [IGVFFI9971JERR](/sequence-files/IGVFFI9971JERR/), [IGVFFI5883JVSV](/sequence-files/IGVFFI5883JVSV/), [IGVFFI4445MXUV](/sequence-files/IGVFFI4445MXUV/), [IGVFFI8974YBIC](/sequence-files/IGVFFI8974YBIC/), [IGVFFI7402YCAH](/sequence-files/IGVFFI7402YCAH/), [IGVFFI8502FHCI](/sequence-files/IGVFFI8502FHCI/), [IGVFFI7332IHPN](/sequence-files/IGVFFI7332IHPN/), [IGVFFI1794CJHH](/sequence-files/IGVFFI1794CJHH/), [IGVFFI2790JGVH](/sequence-files/IGVFFI2790JGVH/), [IGVFFI0175GLUY](/sequence-files/IGVFFI0175GLUY/), [IGVFFI1883CHNE](/sequence-files/IGVFFI1883CHNE/), [IGVFFI5208NQLC](/sequence-files/IGVFFI5208NQLC/), [IGVFFI3235IWKD](/sequence-files/IGVFFI3235IWKD/), [IGVFFI0124EDRT](/sequence-files/IGVFFI0124EDRT/), [IGVFFI8538JXTV](/sequence-files/IGVFFI8538JXTV/), [IGVFFI4603EXCN](/sequence-files/IGVFFI4603EXCN/), [IGVFFI8365GGAJ](/sequence-files/IGVFFI8365GGAJ/), [IGVFFI4962DRXR](/sequence-files/IGVFFI4962DRXR/), [IGVFFI5126QCHA](/sequence-files/IGVFFI5126QCHA/), [IGVFFI7083RAPK](/sequence-files/IGVFFI7083RAPK/), [IGVFFI4844ADFH](/sequence-files/IGVFFI4844ADFH/), [IGVFFI6187WMIZ](/sequence-files/IGVFFI6187WMIZ/), [IGVFFI1609GUEH](/sequence-files/IGVFFI1609GUEH/), [IGVFFI6335CZPE](/sequence-files/IGVFFI6335CZPE/), [IGVFFI1004HOZK](/sequence-files/IGVFFI1004HOZK/), [IGVFFI8851IQMU](/sequence-files/IGVFFI8851IQMU/), [IGVFFI4199GHGW](/sequence-files/IGVFFI4199GHGW/), [IGVFFI1685RQXO](/sequence-files/IGVFFI1685RQXO/) which lack specification of a `sequencing_kit`. Sequence files should specify a sequencing kit.",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_inconsistent_sequencing_kit",
"path": "/measurement-sets/IGVFDS1434CFNM/"
},
{
"category": "missing sequence specification",
"detail": "Measurement set [IGVFDS1434CFNM](/measurement-sets/IGVFDS1434CFNM/) has sequence file(s): [IGVFFI3628PTRU](/sequence-files/IGVFFI3628PTRU/), [IGVFFI8190LTYK](/sequence-files/IGVFFI8190LTYK/), [IGVFFI8296ORHC](/sequence-files/IGVFFI8296ORHC/), [IGVFFI1885EORP](/sequence-files/IGVFFI1885EORP/), [IGVFFI9971JERR](/sequence-files/IGVFFI9971JERR/), [IGVFFI5883JVSV](/sequence-files/IGVFFI5883JVSV/), [IGVFFI4445MXUV](/sequence-files/IGVFFI4445MXUV/), [IGVFFI8974YBIC](/sequence-files/IGVFFI8974YBIC/), [IGVFFI7402YCAH](/sequence-files/IGVFFI7402YCAH/), [IGVFFI8502FHCI](/sequence-files/IGVFFI8502FHCI/), [IGVFFI7332IHPN](/sequence-files/IGVFFI7332IHPN/), [IGVFFI1794CJHH](/sequence-files/IGVFFI1794CJHH/), [IGVFFI2790JGVH](/sequence-files/IGVFFI2790JGVH/), [IGVFFI0175GLUY](/sequence-files/IGVFFI0175GLUY/), [IGVFFI1883CHNE](/sequence-files/IGVFFI1883CHNE/), [IGVFFI5208NQLC](/sequence-files/IGVFFI5208NQLC/), [IGVFFI3235IWKD](/sequence-files/IGVFFI3235IWKD/), [IGVFFI0124EDRT](/sequence-files/IGVFFI0124EDRT/), [IGVFFI8538JXTV](/sequence-files/IGVFFI8538JXTV/), [IGVFFI4603EXCN](/sequence-files/IGVFFI4603EXCN/), [IGVFFI8365GGAJ](/sequence-files/IGVFFI8365GGAJ/), [IGVFFI4962DRXR](/sequence-files/IGVFFI4962DRXR/), [IGVFFI5126QCHA](/sequence-files/IGVFFI5126QCHA/), [IGVFFI7083RAPK](/sequence-files/IGVFFI7083RAPK/), [IGVFFI4844ADFH](/sequence-files/IGVFFI4844ADFH/), [IGVFFI6187WMIZ](/sequence-files/IGVFFI6187WMIZ/), [IGVFFI1609GUEH](/sequence-files/IGVFFI1609GUEH/), [IGVFFI6335CZPE](/sequence-files/IGVFFI6335CZPE/), [IGVFFI1004HOZK](/sequence-files/IGVFFI1004HOZK/), [IGVFFI8851IQMU](/sequence-files/IGVFFI8851IQMU/), [IGVFFI4199GHGW](/sequence-files/IGVFFI4199GHGW/), [IGVFFI1685RQXO](/sequence-files/IGVFFI1685RQXO/) which do not have any `seqspecs`. Sequence files in a file set are expected to link to a sequence specification file.",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_missing_seqspec",
"path": "/measurement-sets/IGVFDS1434CFNM/"
}
],
"WARNING": [
{
"category": "missing auxiliary set",
"detail": "Measurement set [IGVFDS1434CFNM](/measurement-sets/IGVFDS1434CFNM/) has no cell sorting `auxiliary_sets`. CRISPR-based measurement sets that utilize flow cytometry are expected to link to a cell sorting auxiliary set.",
"level": 40,
"level_name": "WARNING",
"name": "audit_missing_auxiliary_set",
"path": "/measurement-sets/IGVFDS1434CFNM/"
}
]
},
"award": {
"@id": "/awards/HG011972/",
"component": "functional characterization",
"contact_pi": {
"@id": "/users/6c75dbfa-635e-4a7a-8ec8-9e51e9c380a4/",
"title": "Jesse Engreitz"
},
"title": "Stanford Center for Connecting DNA Variants to Function and Phenotype"
},
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{
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"accession": "IGVFDS6874YIXA",
"aliases": [
"jesse-engreitz:Jurkat_Stim-IL2RA_prom-NegControl_AmpliconSequencing"
],
"status": "preview"
}
],
"control_for": [],
"creation_timestamp": "2024-11-22T00:47:34.643117+00:00",
"donors": [
{
"@id": "/human-donors/IGVFDO1080XFGV/",
"accession": "IGVFDO1080XFGV",
"aliases": [
"igvf:donor_of_Jurkat"
],
"sex": "male",
"status": "released",
"taxa": "Homo sapiens"
}
],
"externally_hosted": false,
"file_set_type": "experimental data",
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{
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},
{
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},
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},
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},
{
"@id": "/sequence-files/IGVFFI9971JERR/",
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},
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},
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},
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},
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},
{
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"@id": "/users/f9e7cd82-a407-4a20-89ee-f4dc668b0cbe/",
"title": "Michael Montgomery"
},
"submitted_files_timestamp": "2024-11-22T05:48:56.386024+00:00",
"summary": "CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus",
"targeted_genes": [
{
"@id": "/genes/ENSG00000134460/",
"geneid": "ENSG00000134460",
"name": "interleukin 2 receptor subunit alpha",
"status": "released",
"symbol": "IL2RA",
"synonyms": [
"interleukin-2 receptor subunit alpha",
"IL2R",
"TAC antigen",
"IMD41",
"IL-2R subunit alpha",
"CD25",
"IL-2 receptor subunit alpha",
"IDDM10",
"TCGFR",
"p55",
"interleukin 2 receptor, alpha"
]
}
],
"uuid": "1d33572f-86e9-411e-b332-aa5faf04e1e6"
}