Home
Home
Search (⌘K or ⌃K)
Data
Methodology
Data Model
About
Help
Sign In
INDEXED
Home
/
Measurement Set
/
IGVFDS0974QLVO
IGVFDS0974QLVO
released
Json view
File Set Type
experimental data
Summary
cas mediated mutagenesis (Variant FlowFISH) integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
Assay Term
cas mediated mutagenesis
Sample Summaries
Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 51-75% expression of PPIF) modified with a guide library
Construct Library Sets
IGVFDS2385DHZO
Guide (sgRNA) library targeting sequence variants in a genomic locus
Protocols
https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1
Aliases
jesse-engreitz:230423pPPIFEnformer-Jurkat-Amp_PPIF_promoter-BioRep1-FFrep4-BinC_AmpliconSequencing
Samples
1 item
Accession
Type
Sample Terms
Summary
IGVFSM8798UNXX
InVitroSystem
Jurkat
Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 51-75% expression of PPIF) modified with a guide library
Donors
1 item
Accession
Aliases
Sex
Taxa
IGVFDO1080XFGV
igvf:donor_of_Jurkat
male
Homo sapiens
Sequencing Results (Illumina)
48 items
1
2
3
4
5
Accession
File Format
Content Type
Illumina Read Type
Sequencing Run
Associated seqspec Files
Sequencing Platform
Flowcell ID
Lane
Index
File Size
Lab
Upload Status
IGVFFI0107ZPQB
fastq
reads
R2
1
Illumina NextSeq 550
TCCAGCAA:GTCA
13 MB
Jesse Engreitz, Stanford
validated
IGVFFI0124CFMN
fastq
reads
R2
1
Illumina NextSeq 550
TCGGAATG:GTCA
13 MB
Jesse Engreitz, Stanford
validated
IGVFFI1243NKNI
fastq
reads
R1
1
Illumina NextSeq 550
GACCGTTG:GTCA
7.5 MB
Jesse Engreitz, Stanford
validated
IGVFFI1710GPDZ
fastq
reads
R1
1
Illumina NextSeq 550
GCCTAGCC:GTCA
7.9 MB
Jesse Engreitz, Stanford
validated
IGVFFI1810SCSG
fastq
reads
I2
1
Illumina NextSeq 550
GTCGCCTT:GTCA
2.2 MB
Jesse Engreitz, Stanford
validated
IGVFFI2106VZVV
fastq
reads
I2
1
Illumina NextSeq 550
GACCAGGA:GTCA
2.3 MB
Jesse Engreitz, Stanford
validated
IGVFFI2256WABA
fastq
reads
I1
1
Illumina NextSeq 550
CCAGTTAG:GTCA
2.6 MB
Jesse Engreitz, Stanford
validated
IGVFFI2733TORX
fastq
reads
R1
1
Illumina NextSeq 550
GTCGCCTT:GTCA
7.9 MB
Jesse Engreitz, Stanford
validated
IGVFFI2930PAYZ
fastq
reads
I2
1
Illumina NextSeq 550
TCCAGCAA:GTCA
2.3 MB
Jesse Engreitz, Stanford
validated
IGVFFI2952CHMR
fastq
reads
R2
1
Illumina NextSeq 550
CCAGTTAG:GTCA
13 MB
Jesse Engreitz, Stanford
validated
Control File Sets
1 item
Accession
Summary
Aliases
Lab
IGVFDS4869FXYY
cas mediated mutagenesis (Variant FlowFISH)
jesse-engreitz:230423pPPIFEnformer-Jurkat-Amp_PPIF_promoter-Jurkat-PE2_Exp2_NegControl_AmpliconSequencing
Jesse Engreitz, Stanford
Attribution
Award
HG011972
Principal Investigator(s)
Anshul Kundaje
,
Jesse Engreitz
,
Marlene Rabinovitch
,
Lars Steinmetz
,
Michael Bassik
,
Thomas Quertermous
,
Will Greenleaf
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford