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IGVFDS0861MZYT

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This object is in preview status. Metadata is subject to change until this object is released.
File Set Type
experimental data
Summary
prime editing Variant-EFFECTS
Assay Term
in vitro CRISPR screen using flow cytometry
Targeted Genes
Sample Summaries
  • THP-1 cell line, male, Homo sapiens modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2)
Protocols
Aliases
jesse-engreitz:240908-S029-PPIF_5SpliceSitePool-THP1-PE2_Exp6_NegControl_AmpliconSequencing

Sequencing Results (Illumina)

16 items
fastq
reads
I1
1
GTCATCTA:AGGTGCGT
10.0 MB
Jesse Engreitz, Stanford
validated
fastq
reads
I1
1
TTGAATAG:AGGTGCGT
11 MB
Jesse Engreitz, Stanford
validated
fastq
reads
R1
1
CCTTCGCA:AGGTGCGT
35 MB
Jesse Engreitz, Stanford
validated
fastq
reads
R1
1
GTCATCTA:AGGTGCGT
28 MB
Jesse Engreitz, Stanford
validated
fastq
reads
I2
1
TTACGCAC:AGGTGCGT
12 MB
Jesse Engreitz, Stanford
validated
fastq
reads
R2
1
TTGAATAG:AGGTGCGT
29 MB
Jesse Engreitz, Stanford
validated
fastq
reads
R2
1
CCTTCGCA:AGGTGCGT
32 MB
Jesse Engreitz, Stanford
validated
fastq
reads
I2
1
TTGAATAG:AGGTGCGT
10 MB
Jesse Engreitz, Stanford
validated
fastq
reads
I1
1
TTACGCAC:AGGTGCGT
12 MB
Jesse Engreitz, Stanford
validated
fastq
reads
I2
1
GTCATCTA:AGGTGCGT
9.5 MB
Jesse Engreitz, Stanford
validated

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
THP-1 cell line, male, Homo sapiens modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2)

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_THP1
male
Homo sapiens

File Sets Using This Measurement Set as an Input

1 item
Accession
Type
Summary
Aliases
Lab
AnalysisSet
prime editing Variant-EFFECTS targeting PPIF: variant effects
  • jesse-engreitz:240908-S029-PPIF_5SpliceSitePool-THP1_Variant-EFFECTS_analysis
Jesse Engreitz, Stanford

File Sets Controlled by This Measurement Set

20 items
MeasurementSet
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
  • jesse-engreitz:240908-S029-PPIF_5SpliceSitePool-THP1-BioRep2-FFrep1-BinA_AmpliconSequencing
Jesse Engreitz, Stanford
MeasurementSet
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
  • jesse-engreitz:240908-S029-PPIF_5SpliceSitePool-THP1-BioRep1-FFrep1-BinA_AmpliconSequencing
Jesse Engreitz, Stanford
MeasurementSet
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
  • jesse-engreitz:240908-S029-PPIF_5SpliceSitePool-THP1-BioRep2-FFrep2-BinC_AmpliconSequencing
Jesse Engreitz, Stanford
MeasurementSet
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
  • jesse-engreitz:240908-S029-PPIF_5SpliceSitePool-THP1-BioRep1-FFrep1-BinB_AmpliconSequencing
Jesse Engreitz, Stanford
MeasurementSet
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
  • jesse-engreitz:240908-S029-PPIF_5SpliceSitePool-THP1-BioRep1-FFrep1-BinC_AmpliconSequencing
Jesse Engreitz, Stanford
MeasurementSet
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
  • jesse-engreitz:240908-S029-PPIF_5SpliceSitePool-THP1-BioRep1-FFrep2-BinB_AmpliconSequencing
Jesse Engreitz, Stanford
MeasurementSet
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
  • jesse-engreitz:240908-S029-PPIF_5SpliceSitePool-THP1-BioRep1-FFrep2-FACS_Input_AmpliconSequencing
Jesse Engreitz, Stanford
MeasurementSet
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
  • jesse-engreitz:240908-S029-PPIF_5SpliceSitePool-THP1-BioRep2-FFrep2-FACS_Input_AmpliconSequencing
Jesse Engreitz, Stanford
MeasurementSet
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
  • jesse-engreitz:240908-S029-PPIF_5SpliceSitePool-THP1-BioRep1-FFrep2-BinC_AmpliconSequencing
Jesse Engreitz, Stanford
MeasurementSet
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
  • jesse-engreitz:240908-S029-PPIF_5SpliceSitePool-THP1-BioRep2-FFrep1-BinD_AmpliconSequencing
Jesse Engreitz, Stanford

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford