IGVFDS0398KCYH

released

File Set Type

experimental data

Summary

mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

Assay Term

in vitro CRISPR screen using flow cytometry

Targeted Genes

MYC

Sample Summaries

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin B for 10-20% expression of MYC) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library

Construct Library Sets

IGVFDS7857SYCK guide (sgRNA) library targeting accessible genome regions in many genomic loci

Protocols

https://www.protocols.io/view/crispri-of-regulatory-elements-upon-degron-operati-5jyl82d59l2w/v1

Aliases

jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinB-PCR1_Rep1_S26

jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinB-PCR1_Rep2_S81

jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinB-PCR2_Rep1_S32

jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinB-PCR2_Rep2_S87

jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinB-PCR3_Rep1_S38

jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinB-PCR3_Rep2_S93

jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinB-PCR4_Rep1_S44

jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinB-PCR4_Rep2_S99

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM2313EONT

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin B for 10-20% expression of MYC) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library

Donors

1 item

Accession

Aliases

Sex

Taxa

IGVFDO0384OZRW

igvf:donor_of_HCT116

male

Homo sapiens

Auxiliary Datasets

2 items

Files

IGVFDS2834VAYS

AuxiliarySet

gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

IGVFFI7807KLUU, IGVFFI3275WQZQ, IGVFFI4298DZIN, IGVFFI2362LKOQ, IGVFFI5870UCTK, IGVFFI1545RRLD, IGVFFI9245IPZR, IGVFFI9172PREQ

jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinB-PCR1_Rep1_S26
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinB-PCR1_Rep2_S81
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinB-PCR2_Rep1_S32
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinB-PCR2_Rep2_S87
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinB-PCR3_Rep1_S38
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinB-PCR3_Rep2_S93
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinB-PCR4_Rep1_S44
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinB-PCR4_Rep2_S99

Jesse Engreitz, Stanford

IGVFDS8214HXSU

AuxiliarySet

cell sorting for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, ... and 4 more measurement sets

IGVFFI3328SYVP