{
"@context": "/terms/",
"@id": "/measurement-sets/IGVFDS0398KCYH/",
"@type": [
"MeasurementSet",
"FileSet",
"Item"
],
"accession": "IGVFDS0398KCYH",
"aliases": [
"jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinB-PCR1_Rep1_S26",
"jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinB-PCR1_Rep2_S81",
"jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinB-PCR2_Rep1_S32",
"jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinB-PCR2_Rep2_S87",
"jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinB-PCR3_Rep1_S38",
"jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinB-PCR3_Rep2_S93",
"jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinB-PCR4_Rep1_S44",
"jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinB-PCR4_Rep2_S99"
],
"assay_term": {
"@id": "/assay-terms/OBI_0003661/",
"assay_slims": [
"CRISPR screens"
],
"status": "released",
"term_name": "in vitro CRISPR screen using flow cytometry"
},
"audit": {
"INTERNAL_ACTION": [
{
"category": "missing publication",
"detail": "Measurement set [IGVFDS0398KCYH](/measurement-sets/IGVFDS0398KCYH/) has no `publications`. Released and archived file sets are expected to be associated with a publication.",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_file_set_missing_publication",
"path": "/measurement-sets/IGVFDS0398KCYH/"
},
{
"category": "missing sequencing kit",
"detail": "Measurement set [IGVFDS0398KCYH](/measurement-sets/IGVFDS0398KCYH/) has sequence file(s) [IGVFFI4298DZIN](/sequence-files/IGVFFI4298DZIN/), [IGVFFI9172PREQ](/sequence-files/IGVFFI9172PREQ/), [IGVFFI5870UCTK](/sequence-files/IGVFFI5870UCTK/), [IGVFFI9245IPZR](/sequence-files/IGVFFI9245IPZR/), [IGVFFI3275WQZQ](/sequence-files/IGVFFI3275WQZQ/), [IGVFFI7807KLUU](/sequence-files/IGVFFI7807KLUU/), [IGVFFI1545RRLD](/sequence-files/IGVFFI1545RRLD/), [IGVFFI2362LKOQ](/sequence-files/IGVFFI2362LKOQ/) which lack specification of a `sequencing_kit`. Sequence files in a file set associated wtih bulk data should specify a sequencing kit.",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_inconsistent_sequencing_kit",
"path": "/measurement-sets/IGVFDS0398KCYH/"
},
{
"category": "missing publication",
"detail": "Auxiliary set [IGVFDS8214HXSU](/auxiliary-sets/IGVFDS8214HXSU/) has no `publications`. Released and archived file sets are expected to be associated with a publication.",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_file_set_missing_publication",
"path": "/auxiliary-sets/IGVFDS8214HXSU/"
},
{
"category": "missing publication",
"detail": "In vitro system [IGVFSM2313EONT](/in-vitro-systems/IGVFSM2313EONT/) has no `publications`. Released and archived samples are expected to be associated with a publication.",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_sample_missing_publication",
"path": "/in-vitro-systems/IGVFSM2313EONT/"
}
],
"WARNING": [
{
"category": "missing nucleic acid delivery",
"detail": "In vitro system [IGVFSM2313EONT](/in-vitro-systems/IGVFSM2313EONT/) has `construct_library_sets` but is missing `nucleic_acid_delivery`. Samples linked to construct library sets are expected to specify nucleic acid delivery method.",
"level": 40,
"level_name": "WARNING",
"name": "audit_missing_nucleic_acid_delivery",
"path": "/in-vitro-systems/IGVFSM2313EONT/"
}
]
},
"auxiliary_sets": [
{
"@id": "/auxiliary-sets/IGVFDS8214HXSU/",
"accession": "IGVFDS8214HXSU",
"aliases": [
"jesse-engreitz:auxiliary_sortparams_CRUDO_MYC-Auxin0hrs-FF2"
],
"file_set_type": "cell sorting",
"status": "released"
}
],
"award": {
"@id": "/awards/HG011972/",
"component": "functional characterization",
"contact_pi": {
"@id": "/users/6c75dbfa-635e-4a7a-8ec8-9e51e9c380a4/",
"title": "Jesse Engreitz"
},
"title": "Stanford Center for Connecting DNA Variants to Function and Phenotype"
},
"construct_library_sets": [
{
"@id": "/construct-library-sets/IGVFDS7857SYCK/",
"accession": "IGVFDS7857SYCK",
"file_set_type": "guide library",
"integrated_content_files": [
{
"@id": "/tabular-files/IGVFFI3782KCTX/",
"accession": "IGVFFI3782KCTX",
"content_type": "guide RNA sequences",
"status": "released",
"summary": "guide RNA sequences"
}
],
"status": "released",
"summary": "guide (sgRNA) library targeting accessible genome regions in many genomic loci"
}
],
"control_for": [],
"creation_timestamp": "2024-05-21T17:16:09.956677+00:00",
"documents": [
"/documents/74d53b9c-ab1d-42e3-b679-d3a00ae3d842/"
],
"donors": [
{
"@id": "/human-donors/IGVFDO0384OZRW/",
"accession": "IGVFDO0384OZRW",
"aliases": [
"igvf:donor_of_HCT116"
],
"sex": "male",
"status": "released",
"taxa": "Homo sapiens"
}
],
"externally_hosted": false,
"file_set_type": "experimental data",
"files": [
{
"@id": "/configuration-files/IGVFFI6802YWSU/",
"accession": "IGVFFI6802YWSU",
"aliases": [
"jesse-engreitz:seqspecfile_CRUDO_MYC-Auxin0hrs-FF2-BinB-PCR1_Rep2_S81"
],
"content_type": "seqspec",
"creation_timestamp": "2024-06-11T20:47:42.097358+00:00",
"file_format": "yaml",
"file_size": 912,
"href": "/configuration-files/IGVFFI6802YWSU/@@download/IGVFFI6802YWSU.yaml.gz",
"s3_uri": "s3://igvf-files/2024/06/11/eac1decb-293f-417b-bd60-46d6423d7ac0/IGVFFI6802YWSU.yaml.gz",
"status": "released",
"submitted_file_name": "/oak/stanford/groups/engreitz/Projects/Cohesin/240410_IGVF_upload/seqspec/guideseq.out.MYC-Auxin0hrs-FF2-BinB-PCR1_Rep2_S81.yaml",
"upload_status": "validation exempted"
},
{
"@id": "/configuration-files/IGVFFI7331TUOS/",
"accession": "IGVFFI7331TUOS",
"aliases": [
"jesse-engreitz:seqspecfile_CRUDO_MYC-Auxin0hrs-FF2-BinB-PCR4_Rep2_S99"
],
"content_type": "seqspec",
"creation_timestamp": "2024-06-11T20:49:08.624866+00:00",
"file_format": "yaml",
"file_size": 910,
"href": "/configuration-files/IGVFFI7331TUOS/@@download/IGVFFI7331TUOS.yaml.gz",
"s3_uri": "s3://igvf-files/2024/06/11/3c79d8ce-69e9-44ce-9423-024c59b20fb3/IGVFFI7331TUOS.yaml.gz",
"status": "released",
"submitted_file_name": "/oak/stanford/groups/engreitz/Projects/Cohesin/240410_IGVF_upload/seqspec/guideseq.out.MYC-Auxin0hrs-FF2-BinB-PCR4_Rep2_S99.yaml",
"upload_status": "validation exempted"
},
{
"@id": "/configuration-files/IGVFFI2068XJOM/",
"accession": "IGVFFI2068XJOM",
"aliases": [
"jesse-engreitz:seqspecfile_CRUDO_MYC-Auxin0hrs-FF2-BinB-PCR2_Rep1_S32"
],
"content_type": "seqspec",
"creation_timestamp": "2024-06-11T20:47:56.515441+00:00",
"file_format": "yaml",
"file_size": 910,
"href": "/configuration-files/IGVFFI2068XJOM/@@download/IGVFFI2068XJOM.yaml.gz",
"s3_uri": "s3://igvf-files/2024/06/11/3750b49a-d893-4911-8377-8760efc1f015/IGVFFI2068XJOM.yaml.gz",
"status": "released",
"submitted_file_name": "/oak/stanford/groups/engreitz/Projects/Cohesin/240410_IGVF_upload/seqspec/guideseq.out.MYC-Auxin0hrs-FF2-BinB-PCR2_Rep1_S32.yaml",
"upload_status": "validation exempted"
},
{
"@id": "/configuration-files/IGVFFI2350VRGN/",
"accession": "IGVFFI2350VRGN",
"aliases": [
"jesse-engreitz:seqspecfile_CRUDO_MYC-Auxin0hrs-FF2-BinB-PCR3_Rep1_S38"
],
"content_type": "seqspec",
"creation_timestamp": "2024-06-11T20:48:25.173512+00:00",
"file_format": "yaml",
"file_size": 912,
"href": "/configuration-files/IGVFFI2350VRGN/@@download/IGVFFI2350VRGN.yaml.gz",
"s3_uri": "s3://igvf-files/2024/06/11/b3e3988c-e110-4725-8b89-71b4fc31e943/IGVFFI2350VRGN.yaml.gz",
"status": "released",
"submitted_file_name": "/oak/stanford/groups/engreitz/Projects/Cohesin/240410_IGVF_upload/seqspec/guideseq.out.MYC-Auxin0hrs-FF2-BinB-PCR3_Rep1_S38.yaml",
"upload_status": "validation exempted"
},
{
"@id": "/configuration-files/IGVFFI1435OZLL/",
"accession": "IGVFFI1435OZLL",
"aliases": [
"jesse-engreitz:seqspecfile_CRUDO_MYC-Auxin0hrs-FF2-BinB-PCR4_Rep1_S44"
],
"content_type": "seqspec",
"creation_timestamp": "2024-06-11T20:48:53.703128+00:00",
"file_format": "yaml",
"file_size": 911,
"href": "/configuration-files/IGVFFI1435OZLL/@@download/IGVFFI1435OZLL.yaml.gz",
"s3_uri": "s3://igvf-files/2024/06/11/bfbaf361-d9ac-41f6-9aba-99cccb7bea21/IGVFFI1435OZLL.yaml.gz",
"status": "released",
"submitted_file_name": "/oak/stanford/groups/engreitz/Projects/Cohesin/240410_IGVF_upload/seqspec/guideseq.out.MYC-Auxin0hrs-FF2-BinB-PCR4_Rep1_S44.yaml",
"upload_status": "validation exempted"
},
{
"@id": "/configuration-files/IGVFFI8314LAZS/",
"accession": "IGVFFI8314LAZS",
"aliases": [
"jesse-engreitz:seqspecfile_CRUDO_MYC-Auxin0hrs-FF2-BinB-PCR2_Rep2_S87"
],
"content_type": "seqspec",
"creation_timestamp": "2024-06-11T20:48:10.824663+00:00",
"file_format": "yaml",
"file_size": 911,
"href": "/configuration-files/IGVFFI8314LAZS/@@download/IGVFFI8314LAZS.yaml.gz",
"s3_uri": "s3://igvf-files/2024/06/11/2ae8e151-b334-4c01-a022-e3b70ce98657/IGVFFI8314LAZS.yaml.gz",
"status": "released",
"submitted_file_name": "/oak/stanford/groups/engreitz/Projects/Cohesin/240410_IGVF_upload/seqspec/guideseq.out.MYC-Auxin0hrs-FF2-BinB-PCR2_Rep2_S87.yaml",
"upload_status": "validation exempted"
},
{
"@id": "/sequence-files/IGVFFI4298DZIN/",
"accession": "IGVFFI4298DZIN",
"aliases": [
"jesse-engreitz:auxiliary_file_CRUDO_MYC-Auxin0hrs-FF2-BinB-PCR3_Rep1_S38"
],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2024-06-01T02:02:15.190391+00:00",
"file_format": "fastq",
"file_size": 34006187,
"href": "/sequence-files/IGVFFI4298DZIN/@@download/IGVFFI4298DZIN.fastq.gz",
"s3_uri": "s3://igvf-files/2024/06/01/e695ffd0-0f68-4feb-92d3-b1770d15935a/IGVFFI4298DZIN.fastq.gz",
"sequencing_platform": {
"@id": "/platform-terms/EFO_0008565/",
"status": "released",
"term_name": "Illumina HiSeq 2500"
},
"status": "released",
"submitted_file_name": "/oak/stanford/groups/engreitz/Projects/Cohesin/220819B015_MYC/fastq_trim/MYC-Auxin0hrs-FF2-BinB-PCR3-Rep1_S38_R1_001.fastq.gz",
"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI9172PREQ/",
"accession": "IGVFFI9172PREQ",
"aliases": [
"jesse-engreitz:auxiliary_file_CRUDO_MYC-Auxin0hrs-FF2-BinB-PCR4_Rep2_S99"
],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2024-06-01T02:03:17.734658+00:00",
"file_format": "fastq",
"file_size": 12225763,
"href": "/sequence-files/IGVFFI9172PREQ/@@download/IGVFFI9172PREQ.fastq.gz",
"s3_uri": "s3://igvf-files/2024/06/01/a610ec8a-5fe6-4775-8f72-919eb966a6b8/IGVFFI9172PREQ.fastq.gz",
"sequencing_platform": {
"@id": "/platform-terms/EFO_0008565/",
"status": "released",
"term_name": "Illumina HiSeq 2500"
},
"status": "released",
"submitted_file_name": "/oak/stanford/groups/engreitz/Projects/Cohesin/220819B015_MYC/fastq_trim/MYC-Auxin0hrs-FF2-BinB-PCR4-Rep2_S99_R1_001.fastq.gz",
"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI5870UCTK/",
"accession": "IGVFFI5870UCTK",
"aliases": [
"jesse-engreitz:auxiliary_file_CRUDO_MYC-Auxin0hrs-FF2-BinB-PCR2_Rep1_S32"
],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2024-06-01T02:01:33.300910+00:00",
"file_format": "fastq",
"file_size": 45867427,
"href": "/sequence-files/IGVFFI5870UCTK/@@download/IGVFFI5870UCTK.fastq.gz",
"s3_uri": "s3://igvf-files/2024/06/01/f75ed6e6-66d6-4fd7-b3a8-2c635f43d0ef/IGVFFI5870UCTK.fastq.gz",
"sequencing_platform": {
"@id": "/platform-terms/EFO_0008565/",
"status": "released",
"term_name": "Illumina HiSeq 2500"
},
"status": "released",
"submitted_file_name": "/oak/stanford/groups/engreitz/Projects/Cohesin/220819B015_MYC/fastq_trim/MYC-Auxin0hrs-FF2-BinB-PCR2-Rep1_S32_R1_001.fastq.gz",
"upload_status": "validated"
},
{
"@id": "/configuration-files/IGVFFI8168MZFJ/",
"accession": "IGVFFI8168MZFJ",
"aliases": [
"jesse-engreitz:seqspecfile_CRUDO_MYC-Auxin0hrs-FF2-BinB-PCR1_Rep1_S26"
],
"content_type": "seqspec",
"creation_timestamp": "2024-06-11T20:47:27.584218+00:00",
"file_format": "yaml",
"file_size": 911,
"href": "/configuration-files/IGVFFI8168MZFJ/@@download/IGVFFI8168MZFJ.yaml.gz",
"s3_uri": "s3://igvf-files/2024/06/11/f7361e23-06bf-4c80-a098-2519c2603519/IGVFFI8168MZFJ.yaml.gz",
"status": "released",
"submitted_file_name": "/oak/stanford/groups/engreitz/Projects/Cohesin/240410_IGVF_upload/seqspec/guideseq.out.MYC-Auxin0hrs-FF2-BinB-PCR1_Rep1_S26.yaml",
"upload_status": "validation exempted"
},
{
"@id": "/configuration-files/IGVFFI2467IQLT/",
"accession": "IGVFFI2467IQLT",
"aliases": [
"jesse-engreitz:seqspecfile_CRUDO_MYC-Auxin0hrs-FF2-BinB-PCR3_Rep2_S93"
],
"content_type": "seqspec",
"creation_timestamp": "2024-06-11T20:48:39.216286+00:00",
"file_format": "yaml",
"file_size": 911,
"href": "/configuration-files/IGVFFI2467IQLT/@@download/IGVFFI2467IQLT.yaml.gz",
"s3_uri": "s3://igvf-files/2024/06/11/d741966c-c596-49a2-b94c-97d7eb4808ac/IGVFFI2467IQLT.yaml.gz",
"status": "released",
"submitted_file_name": "/oak/stanford/groups/engreitz/Projects/Cohesin/240410_IGVF_upload/seqspec/guideseq.out.MYC-Auxin0hrs-FF2-BinB-PCR3_Rep2_S93.yaml",
"upload_status": "validation exempted"
},
{
"@id": "/sequence-files/IGVFFI9245IPZR/",
"accession": "IGVFFI9245IPZR",
"aliases": [
"jesse-engreitz:auxiliary_file_CRUDO_MYC-Auxin0hrs-FF2-BinB-PCR3_Rep2_S93"
],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2024-06-01T02:02:35.609914+00:00",
"file_format": "fastq",
"file_size": 8858294,
"href": "/sequence-files/IGVFFI9245IPZR/@@download/IGVFFI9245IPZR.fastq.gz",
"s3_uri": "s3://igvf-files/2024/06/01/8f5eab92-9563-45e0-8044-486283f62377/IGVFFI9245IPZR.fastq.gz",
"sequencing_platform": {
"@id": "/platform-terms/EFO_0008565/",
"status": "released",
"term_name": "Illumina HiSeq 2500"
},
"status": "released",
"submitted_file_name": "/oak/stanford/groups/engreitz/Projects/Cohesin/220819B015_MYC/fastq_trim/MYC-Auxin0hrs-FF2-BinB-PCR3-Rep2_S93_R1_001.fastq.gz",
"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI3275WQZQ/",
"accession": "IGVFFI3275WQZQ",
"aliases": [
"jesse-engreitz:auxiliary_file_CRUDO_MYC-Auxin0hrs-FF2-BinB-PCR1_Rep2_S81"
],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2024-06-01T02:01:08.892229+00:00",
"file_format": "fastq",
"file_size": 28552104,
"href": "/sequence-files/IGVFFI3275WQZQ/@@download/IGVFFI3275WQZQ.fastq.gz",
"s3_uri": "s3://igvf-files/2024/06/01/ca9629bd-aead-4685-8c24-0d14830cf977/IGVFFI3275WQZQ.fastq.gz",
"sequencing_platform": {
"@id": "/platform-terms/EFO_0008565/",
"status": "released",
"term_name": "Illumina HiSeq 2500"
},
"status": "released",
"submitted_file_name": "/oak/stanford/groups/engreitz/Projects/Cohesin/220819B015_MYC/fastq_trim/MYC-Auxin0hrs-FF2-BinB-PCR1-Rep2_S81_R1_001.fastq.gz",
"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI7807KLUU/",
"accession": "IGVFFI7807KLUU",
"aliases": [
"jesse-engreitz:auxiliary_file_CRUDO_MYC-Auxin0hrs-FF2-BinB-PCR1_Rep1_S26"
],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2024-06-01T02:00:48.191718+00:00",
"file_format": "fastq",
"file_size": 52386666,
"href": "/sequence-files/IGVFFI7807KLUU/@@download/IGVFFI7807KLUU.fastq.gz",
"s3_uri": "s3://igvf-files/2024/06/01/46576688-e224-4930-ad48-e405c210bbcb/IGVFFI7807KLUU.fastq.gz",
"sequencing_platform": {
"@id": "/platform-terms/EFO_0008565/",
"status": "released",
"term_name": "Illumina HiSeq 2500"
},
"status": "released",
"submitted_file_name": "/oak/stanford/groups/engreitz/Projects/Cohesin/220819B015_MYC/fastq_trim/MYC-Auxin0hrs-FF2-BinB-PCR1-Rep1_S26_R1_001.fastq.gz",
"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI1545RRLD/",
"accession": "IGVFFI1545RRLD",
"aliases": [
"jesse-engreitz:auxiliary_file_CRUDO_MYC-Auxin0hrs-FF2-BinB-PCR2_Rep2_S87"
],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2024-06-01T02:01:53.919516+00:00",
"file_format": "fastq",
"file_size": 28097455,
"href": "/sequence-files/IGVFFI1545RRLD/@@download/IGVFFI1545RRLD.fastq.gz",
"s3_uri": "s3://igvf-files/2024/06/01/9b6c6ef0-bf75-42bf-bea0-3196f183148d/IGVFFI1545RRLD.fastq.gz",
"sequencing_platform": {
"@id": "/platform-terms/EFO_0008565/",
"status": "released",
"term_name": "Illumina HiSeq 2500"
},
"status": "released",
"submitted_file_name": "/oak/stanford/groups/engreitz/Projects/Cohesin/220819B015_MYC/fastq_trim/MYC-Auxin0hrs-FF2-BinB-PCR2-Rep2_S87_R1_001.fastq.gz",
"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI2362LKOQ/",
"accession": "IGVFFI2362LKOQ",
"aliases": [
"jesse-engreitz:auxiliary_file_CRUDO_MYC-Auxin0hrs-FF2-BinB-PCR4_Rep1_S44"
],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2024-06-01T02:02:56.247963+00:00",
"file_format": "fastq",
"file_size": 38235310,
"href": "/sequence-files/IGVFFI2362LKOQ/@@download/IGVFFI2362LKOQ.fastq.gz",
"s3_uri": "s3://igvf-files/2024/06/01/1bd71244-8bdc-447b-9b8a-a3c8995ee2a3/IGVFFI2362LKOQ.fastq.gz",
"sequencing_platform": {
"@id": "/platform-terms/EFO_0008565/",
"status": "released",
"term_name": "Illumina HiSeq 2500"
},
"status": "released",
"submitted_file_name": "/oak/stanford/groups/engreitz/Projects/Cohesin/220819B015_MYC/fastq_trim/MYC-Auxin0hrs-FF2-BinB-PCR4-Rep1_S44_R1_001.fastq.gz",
"upload_status": "validated"
}
],
"input_for": [
"/analysis-sets/IGVFDS4544YEQM/"
],
"lab": {
"@id": "/labs/jesse-engreitz/",
"title": "Jesse Engreitz, Stanford"
},
"notes": "The readout 2e41e624-f6c7-4f0c-a07e-bc36cefdca39 was removed from this measurement set.",
"preferred_assay_title": "CRISPR FlowFISH screen",
"protocols": [
"https://www.protocols.io/view/crispri-of-regulatory-elements-upon-degron-operati-5jyl82d59l2w/v1"
],
"release_timestamp": "2024-07-10T19:47:00.392394+00:00",
"samples": [
{
"@id": "/in-vitro-systems/IGVFSM2313EONT/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM2313EONT",
"aliases": [
"jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin0hrs-FF2-BinB-PCR1_Rep1_S26",
"jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin0hrs-FF2-BinB-PCR1_Rep2_S81",
"jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin0hrs-FF2-BinB-PCR2_Rep1_S32",
"jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin0hrs-FF2-BinB-PCR2_Rep2_S87",
"jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin0hrs-FF2-BinB-PCR3_Rep1_S38",
"jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin0hrs-FF2-BinB-PCR3_Rep2_S93",
"jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin0hrs-FF2-BinB-PCR4_Rep1_S44",
"jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin0hrs-FF2-BinB-PCR4_Rep2_S99"
],
"classifications": [
"cell line"
],
"institutional_certificates": [],
"modifications": [
{
"@id": "/crispr-modifications/8543fa06-1720-4e7d-8e8f-7e24c1b1cf79/",
"modality": "interference",
"status": "released"
},
{
"@id": "/degron-modifications/2e8d10cc-fc98-44d5-a7d9-e2892e16c98a/",
"modality": "degradation",
"status": "released"
}
],
"sample_terms": [
{
"@id": "/sample-terms/EFO_0002824/",
"@type": [
"SampleTerm",
"OntologyTerm",
"Item"
],
"aliases": [
"igvf:HCT116"
],
"status": "released",
"summary": "HCT116",
"term_name": "HCT116"
}
],
"status": "released",
"summary": "Homo sapiens (male) HCT116 cell line (sorting details: FACS bin B for 10-20% expression of MYC) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library",
"taxa": "Homo sapiens"
}
],
"schema_version": "26",
"status": "released",
"submitted_by": {
"@id": "/users/3bd6f74c-8cf3-4c35-a94d-0c7c11654815/",
"title": "Chongyon Park"
},
"submitted_files_timestamp": "2024-06-01T02:00:48.191718+00:00",
"summary": "mixed CRISPR FlowFISH screen sorted on the expression of MYC integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci",
"targeted_genes": [
{
"@id": "/genes/ENSG00000136997/",
"geneid": "ENSG00000136997",
"name": "MYC proto-oncogene, bHLH transcription factor",
"status": "released",
"symbol": "MYC",
"synonyms": [
"bHLHe39",
"myc-related translation/localization regulatory factor",
"MYC proto-oncogene, bHLH transcription factor",
"MYC",
"c-Myc",
"v-myc avian myelocytomatosis viral oncogene homolog",
"proto-oncogene c-Myc",
"transcription factor p64",
"myc proto-oncogene protein",
"avian myelocytomatosis viral oncogene homolog",
"MRTL",
"class E basic helix-loop-helix protein 39",
"MYCC",
"v-myc myelocytomatosis viral oncogene homolog"
]
}
],
"uuid": "b2087f5b-0f1c-46d4-b4ce-9c48f6e21e26"
}