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IGVFFI6124CLAF

released
validated
File Set
IGVFDS5239SBBT(CRISPR activation Perturb-seq integrating a guide (sgRNA) library targeting transcription start sites in 24 genes)
Summary
gene by gene expression by CRISPR guide capture in filtered feature barcode matrix
Analysis Step Version
/analysis-step-versions/fbd5e8fb-4206-4b43-92d6-922cdfe1338b/
File Format
tar
Content Type
filtered feature barcode matrix
Content Summary
gene by gene expression by CRISPR guide capture in filtered feature barcode matrix
Aliases
charles-gersbach:cd8-tcells-perturb-seq-10x-5prime-mm-crispra-rep3
md5sum
1ae0ad3f7ceefd4397a6351e3f498143
Content MD5sum
1f275d26bde213b5a8e512bb0a71b81d
File Size
336 MB
Submitted File Name
/data/reddylab/Alex/IGVF/data_submissions/processed_data/AIM_2_T_cell_project_1/CRISPRa_D3_10x.tar.gz
Checkfiles Version
Legacy version of checkfiles
Submitter Comment
Cell Ranger Count Pipeline run with chemistry argument 'SC5P-R2'
Attribution
Charles Gersbach, Duke

Matrix Details

Principal Dimension
gene
Secondary Dimensions
gene expression, CRISPR guide capture

Samples

3 items
Sample Terms
Summary
PrimaryCell
Homo sapiens CD8-positive, alpha-beta memory T cell modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
Homo sapiens CD8-positive, alpha-beta memory T cell modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
Homo sapiens CD8-positive, alpha-beta memory T cell modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)

Reference Files

1 item
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
gtf
transcriptome reference
GENCODE 32 transcriptome reference
External Lab, Community
43 MB
validated

Files This File Derives From

16 items
fastq
reads
Charles Gersbach, Duke
1.3 GB
validated
fastq
reads
Charles Gersbach, Duke
2.5 GB
validated
fastq
reads
Charles Gersbach, Duke
1.3 GB
validated
fastq
reads
Charles Gersbach, Duke
1.3 GB
validated
fastq
reads
Charles Gersbach, Duke
2.5 GB
validated
fastq
reads
Charles Gersbach, Duke
1.3 GB
validated
fastq
reads
Charles Gersbach, Duke
4.6 GB
validated
fastq
reads
Charles Gersbach, Duke
4.6 GB
validated
fastq
reads
Charles Gersbach, Duke
11 GB
validated
fastq
reads
Charles Gersbach, Duke
4.6 GB
validated

Files Derived From This File

1 item
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
tsv
differential gene expression quantifications
GRCh38 differential gene expression quantifications
Charles Gersbach, Duke
23 MB
validated