{
"@context": "/terms/",
"@id": "/matrix-files/IGVFFI6124CLAF/",
"@type": [
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"File",
"Item"
],
"accession": "IGVFFI6124CLAF",
"aliases": [
"charles-gersbach:cd8-tcells-perturb-seq-10x-5prime-mm-crispra-rep3"
],
"analysis_step_version": "/analysis-step-versions/fbd5e8fb-4206-4b43-92d6-922cdfe1338b/",
"assay_titles": [
"Perturb-seq"
],
"audit": {},
"award": {
"@id": "/awards/HG012053/",
"component": "functional characterization"
},
"content_md5sum": "1f275d26bde213b5a8e512bb0a71b81d",
"content_summary": "gene by gene expression by CRISPR guide capture in filtered feature barcode matrix",
"content_type": "filtered feature barcode matrix",
"creation_timestamp": "2024-09-22T17:44:52.028404+00:00",
"derived_from": [
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"/sequence-files/IGVFFI6787BWWZ/",
"/sequence-files/IGVFFI7868FNQC/",
"/sequence-files/IGVFFI7249VOBK/",
"/sequence-files/IGVFFI6398QCVG/",
"/sequence-files/IGVFFI6606ARBS/",
"/sequence-files/IGVFFI4462ZXLC/",
"/sequence-files/IGVFFI8501DMZJ/",
"/sequence-files/IGVFFI1328IASZ/",
"/sequence-files/IGVFFI2032UXJP/",
"/sequence-files/IGVFFI3786SYLP/",
"/sequence-files/IGVFFI7135VSEK/",
"/sequence-files/IGVFFI2506CIAV/",
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"/sequence-files/IGVFFI8543ICNA/"
],
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{
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"accession": "IGVFSM8036VPRU",
"classifications": [
"primary cell"
],
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{
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"modality": "activation",
"summary": "CRISPRa Sa-dCas9-VP64"
}
],
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)",
"taxa": "Homo sapiens"
},
{
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],
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{
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"summary": "CRISPRa Sa-dCas9-VP64"
}
],
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)",
"taxa": "Homo sapiens"
},
{
"@id": "/primary-cells/IGVFSM6223YCQV/",
"accession": "IGVFSM6223YCQV",
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],
"modifications": [
{
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"modality": "activation",
"summary": "CRISPRa Sa-dCas9-VP64"
}
],
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)",
"taxa": "Homo sapiens"
}
],
"summary": "activation Perturb-seq: filtered feature barcode matrix"
},
"file_size": 336384580,
"gene_list_for": [],
"href": "/matrix-files/IGVFFI6124CLAF/@@download/IGVFFI6124CLAF.tar.gz",
"input_file_for": [
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],
"integrated_in": [],
"lab": {
"@id": "/labs/charles-gersbach/",
"title": "Charles Gersbach, Duke"
},
"loci_list_for": [],
"md5sum": "1ae0ad3f7ceefd4397a6351e3f498143",
"principal_dimension": "gene",
"reference_files": [
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],
"release_timestamp": "2024-10-18T19:49:58.634906+00:00",
"s3_uri": "s3://igvf-files/2024/09/22/d1860ce5-49fc-462c-9521-d7ca898c24e7/IGVFFI6124CLAF.tar.gz",
"schema_version": "7",
"secondary_dimensions": [
"gene expression",
"CRISPR guide capture"
],
"status": "released",
"submitted_by": {
"@id": "/users/29f5f78d-ac90-421e-90c6-a90e1dc407a7/",
"title": "Alejandro Barrera"
},
"submitted_file_name": "/data/reddylab/Alex/IGVF/data_submissions/processed_data/AIM_2_T_cell_project_1/CRISPRa_D3_10x.tar.gz",
"submitter_comment": "Cell Ranger Count Pipeline run with chemistry argument 'SC5P-R2'",
"summary": "gene by gene expression by CRISPR guide capture in filtered feature barcode matrix",
"upload_status": "validated",
"uuid": "d1860ce5-49fc-462c-9521-d7ca898c24e7"
}