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IGVFFI9664FBRY
IGVFFI9664FBRY
released
validated
Json view
File Set
IGVFDS9674AATW
(CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting transcription start sites in 24 genes)
Summary
seqspec of IGVFFI8510AXNZ, IGVFFI3515IWOR
File Format
yaml
Content Type
seqspec
Aliases
charles-gersbach:cd8-tcells-perturb-seq-crispri-gex-rep3-rep3-fastq-lane2_seqspec
md5sum
3d3c2e82bc7c442d4aff3eb8d09b6e04
Content MD5sum
cb6413e443cec7aec86616a9c39b7f53
File Size
982 B
Submitted File Name
/datacommons/gersbachlab/Ruhi/IGVFseqspec/CRISPRiD3_S3nS9_L002.rna.yaml.gz
seqspec File Of
2 items
Accession
File Format
Content Type
Sequencing Run
Sequencing Platform
Flowcell ID
Lane
Index
File Size
Lab
Upload Status
IGVFFI3515IWOR
fastq
reads
3
Illumina NovaSeq 6000
2
5.7 GB
Charles Gersbach, Duke
validated
IGVFFI8510AXNZ
fastq
reads
3
Illumina NovaSeq 6000
2
14 GB
Charles Gersbach, Duke
validated
Samples
1 item
Accession
Type
Sample Terms
Summary
IGVFSM5189UBOI
PrimaryCell
CD8-positive, alpha-beta memory T cell
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)
Attribution
Award
HG012053
Principal Investigator(s)
Charles Gersbach
,
Gregory Crawford
,
Tim Reddy
Contact P.I.
Charles Gersbach
Lab
Charles Gersbach, Duke