{
"@context": "/terms/",
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"@type": [
"ConfigurationFile",
"File",
"Item"
],
"accession": "IGVFFI9664FBRY",
"aliases": [
"charles-gersbach:cd8-tcells-perturb-seq-crispri-gex-rep3-rep3-fastq-lane2_seqspec"
],
"assay_titles": [
"Perturb-seq"
],
"audit": {},
"award": {
"@id": "/awards/HG012053/",
"component": "functional characterization"
},
"content_md5sum": "cb6413e443cec7aec86616a9c39b7f53",
"content_type": "seqspec",
"creation_timestamp": "2024-09-20T16:49:35.935019+00:00",
"file_format": "yaml",
"file_set": {
"@id": "/measurement-sets/IGVFDS9674AATW/",
"accession": "IGVFDS9674AATW",
"file_set_type": "experimental data",
"samples": [
{
"@id": "/primary-cells/IGVFSM5189UBOI/",
"accession": "IGVFSM5189UBOI",
"classifications": [
"primary cell"
],
"modifications": [
{
"@id": "/crispr-modifications/95393c9a-637d-43f6-baf9-662e45d28d61/",
"modality": "interference",
"status": "released",
"summary": "CRISPRi Sa-dCas9"
}
],
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"status": "released",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"status": "released",
"summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)",
"taxa": "Homo sapiens"
}
],
"status": "released",
"summary": "CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting transcription start sites in 24 genes"
},
"file_size": 982,
"gene_list_for": [],
"href": "/configuration-files/IGVFFI9664FBRY/@@download/IGVFFI9664FBRY.yaml.gz",
"input_file_for": [],
"integrated_in": [],
"lab": {
"@id": "/labs/charles-gersbach/",
"title": "Charles Gersbach, Duke"
},
"loci_list_for": [],
"md5sum": "3d3c2e82bc7c442d4aff3eb8d09b6e04",
"release_timestamp": "2024-10-18T19:50:07.413824+00:00",
"s3_uri": "s3://igvf-files/2024/09/20/90af4e1f-3eec-4dde-838d-97a03d35cca3/IGVFFI9664FBRY.yaml.gz",
"schema_version": "7",
"seqspec_of": [
"/sequence-files/IGVFFI8510AXNZ/",
"/sequence-files/IGVFFI3515IWOR/"
],
"status": "released",
"submitted_by": {
"@id": "/users/3782ce69-0e9f-4b46-91f9-4578139785c7/",
"title": "Ruhi Rai"
},
"submitted_file_name": "/datacommons/gersbachlab/Ruhi/IGVFseqspec/CRISPRiD3_S3nS9_L002.rna.yaml.gz",
"summary": "seqspec of IGVFFI8510AXNZ, IGVFFI3515IWOR",
"upload_status": "validated",
"uuid": "90af4e1f-3eec-4dde-838d-97a03d35cca3"
}