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IGVFFI1298SMWW
IGVFFI1298SMWW
released
validated
Json view
File Set
IGVFDS8029DSPH
(gRNA sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting transcription start sites in 24 genes)
Summary
seqspec of IGVFFI9522NHQX, IGVFFI5512GZXV
File Format
yaml
Content Type
seqspec
Aliases
charles-gersbach:cd8-tcells-perturb-seq-crispri-grna-rep1-rep1-fastq-lane4_seqspec
md5sum
20042dd0c2d4f2ba5d3c66176cb68553
Content MD5sum
0c66dccc52888382547ed066b4f81e9b
File Size
1.1 KB
Submitted File Name
/datacommons/gersbachlab/Ruhi/IGVFseqspec/CRISPRiD1_S1nS7_L004.crispr.yaml.gz
seqspec File Of
2 items
Accession
File Format
Content Type
Sequencing Run
Sequencing Platform
Flowcell ID
Lane
Index
File Size
Lab
Upload Status
IGVFFI5512GZXV
fastq
reads
1
Illumina NovaSeq 6000
HMCCWDSX3
4
4.4 GB
Charles Gersbach, Duke
validated
IGVFFI9522NHQX
fastq
reads
1
Illumina NovaSeq 6000
HMCCWDSX3
4
2.5 GB
Charles Gersbach, Duke
validated
Samples
1 item
Accession
Type
Sample Terms
Summary
IGVFSM3795JDHP
PrimaryCell
CD8-positive, alpha-beta memory T cell
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)
Attribution
Award
HG012053
Principal Investigator(s)
Charles Gersbach
,
Gregory Crawford
,
Tim Reddy
Contact P.I.
Charles Gersbach
Lab
Charles Gersbach, Duke