IGVFFI1298SMWW

{
    "@context": "/terms/",
    "@id": "/configuration-files/IGVFFI1298SMWW/",
    "@type": [
        "ConfigurationFile",
        "File",
        "Item"
    ],
    "accession": "IGVFFI1298SMWW",
    "aliases": [
        "charles-gersbach:cd8-tcells-perturb-seq-crispri-grna-rep1-rep1-fastq-lane4_seqspec"
    ],
    "assay_titles": [
        "Perturb-seq"
    ],
    "audit": {},
    "award": {
        "@id": "/awards/HG012053/",
        "component": "functional characterization"
    },
    "content_md5sum": "0c66dccc52888382547ed066b4f81e9b",
    "content_type": "seqspec",
    "creation_timestamp": "2024-09-20T16:50:15.443055+00:00",
    "file_format": "yaml",
    "file_set": {
        "@id": "/auxiliary-sets/IGVFDS8029DSPH/",
        "accession": "IGVFDS8029DSPH",
        "file_set_type": "gRNA sequencing",
        "samples": [
            {
                "@id": "/primary-cells/IGVFSM3795JDHP/",
                "accession": "IGVFSM3795JDHP",
                "classifications": [
                    "primary cell"
                ],
                "sample_terms": [
                    {
                        "@id": "/sample-terms/CL_0000909/",
                        "term_name": "CD8-positive, alpha-beta memory T cell"
                    }
                ],
                "summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)",
                "taxa": "Homo sapiens"
            }
        ],
        "summary": "gRNA sequencing for interference Perturb-seq integrating a guide (sgRNA) library targeting transcription start sites in 24 genes"
    },
    "file_size": 1080,
    "gene_list_for": [],
    "href": "/configuration-files/IGVFFI1298SMWW/@@download/IGVFFI1298SMWW.yaml.gz",
    "input_file_for": [],
    "integrated_in": [],
    "lab": {
        "@id": "/labs/charles-gersbach/",
        "title": "Charles Gersbach, Duke"
    },
    "loci_list_for": [],
    "md5sum": "20042dd0c2d4f2ba5d3c66176cb68553",
    "release_timestamp": "2024-10-18T19:50:06.313757+00:00",
    "s3_uri": "s3://igvf-files/2024/09/20/5713ee32-c858-4801-9fa2-7671fa844299/IGVFFI1298SMWW.yaml.gz",
    "schema_version": "7",
    "seqspec_of": [
        "/sequence-files/IGVFFI9522NHQX/",
        "/sequence-files/IGVFFI5512GZXV/"
    ],
    "status": "released",
    "submitted_by": {
        "@id": "/users/3782ce69-0e9f-4b46-91f9-4578139785c7/",
        "title": "Ruhi Rai"
    },
    "submitted_file_name": "/datacommons/gersbachlab/Ruhi/IGVFseqspec/CRISPRiD1_S1nS7_L004.crispr.yaml.gz",
    "summary": "seqspec of IGVFFI9522NHQX, IGVFFI5512GZXV",
    "upload_status": "validated",
    "uuid": "5713ee32-c858-4801-9fa2-7671fa844299"
}