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IGVFDS9906UGWB

released
Aliases
charles-gersbach:cd8-tcells-analysis-set-rna-seq-cas-ko-znf217
File Set Type
principal analysis
Summary
knockout RNA-seq: fold change over control, transcript quantifications, transcriptome alignments
Workflows
Publications
Samples
CD8-positive, alpha-beta memory T cell primary cell, modified with CRISPR modifications, transfected with a guide library
Protocols

Files

5 items
bam
transcriptome alignments
GRCh38 filtered transcriptome alignments
Charles Gersbach, Duke
2.8 GB
filtered
validated
bam
transcriptome alignments
GRCh38 filtered transcriptome alignments
Charles Gersbach, Duke
2.2 GB
filtered
validated
tsv
fold change over control
GRCh38 fold change over control
Charles Gersbach, Duke
852 KB
validated
bam
transcriptome alignments
GRCh38 filtered transcriptome alignments
Charles Gersbach, Duke
2.3 GB
filtered
validated
tsv
transcript quantifications
GRCh38 transcript quantifications
Charles Gersbach, Duke
549 KB
validated

File Association Graph

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File Set
File
AnalysisSet
MeasurementSet

Samples

6 items
Sample Terms
Associated Construct Library Sets
Summary
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)

Donors

3 items
Aliases
  • charles-gersbach:cd8-tcells-donor-14
unspecified
Homo sapiens
  • charles-gersbach:cd8-tcells-donor-13
unspecified
Homo sapiens
  • charles-gersbach:cd8-tcells-donor-12
unspecified
Homo sapiens

Associated Construct Library Sets

2 items
Summary
Library Design Files
guide (sgRNA) library targeting transcription start sites in ZNF217
non-targeting guide (sgRNA) library

MeasurementSet Input File Sets

3 items
Controls
CRISPR knockout RNA-seq integrating a guide (sgRNA) library targeting transcription start sites in ZNF217
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)
CRISPR knockout RNA-seq integrating a guide (sgRNA) library targeting transcription start sites in ZNF217
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)
CRISPR knockout RNA-seq integrating a guide (sgRNA) library targeting transcription start sites in ZNF217
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)

AnalysisSet Input File Sets

1 item
Accession
Summary
knockout RNA-seq: transcriptome alignments

Attribution

Award
HG012053
Principal Investigator(s)
Contact P.I.
Charles Gersbach
Lab
Charles Gersbach, Duke