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IGVFDS9906UGWB

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Aliases
charles-gersbach:cd8-tcells-analysis-set-rna-seq-cas-ko-znf217
File Set Type
principal analysis
Summary
CRISPR knockout RNA-seq integrating a guide (sgRNA) library targeting transcription start sites in ZNF217 and a non-targeting guide (sgRNA) library with non-targeting control
Publications
Samples
Homo sapiens CD8-positive, alpha-beta memory T primary cell, modified with CRISPRko Sp-Cas9, transfected with a guide library
Protocols
Attribution
Charles Gersbach, Duke

Files

5 items
bam
transcriptome alignments
GRCh38 filtered transcriptome alignments
Charles Gersbach, Duke
2.8 GB
filtered
validated
bam
transcriptome alignments
GRCh38 filtered transcriptome alignments
Charles Gersbach, Duke
2.2 GB
filtered
validated
tsv
fold change over control
GRCh38 fold change over control
Charles Gersbach, Duke
852 KB
validated
bam
transcriptome alignments
GRCh38 filtered transcriptome alignments
Charles Gersbach, Duke
2.3 GB
filtered
validated
tsv
transcript quantifications
GRCh38 transcript quantifications
Charles Gersbach, Duke
549 KB
validated

File Association Graph

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File Set
File
AnalysisSet
MeasurementSet

Samples

6 items
Sample Terms
Associated Construct Library Sets
Summary
PrimaryCell
Homo sapiens CD8-positive, alpha-beta memory T cell modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
Homo sapiens CD8-positive, alpha-beta memory T cell modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
Homo sapiens CD8-positive, alpha-beta memory T cell modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
Homo sapiens CD8-positive, alpha-beta memory T cell modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
Homo sapiens CD8-positive, alpha-beta memory T cell modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
Homo sapiens CD8-positive, alpha-beta memory T cell modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)

Donors

3 items
Aliases
  • charles-gersbach:cd8-tcells-donor-14
unspecified
Homo sapiens
  • charles-gersbach:cd8-tcells-donor-13
unspecified
Homo sapiens
  • charles-gersbach:cd8-tcells-donor-12
unspecified
Homo sapiens

Associated Construct Library Sets

2 items
Summary
Library Design Files
guide (sgRNA) library targeting transcription start sites in ZNF217
non-targeting guide (sgRNA) library

MeasurementSet Input File Sets

3 items
Controls
CRISPR knockout RNA-seq integrating a guide (sgRNA) library targeting transcription start sites in ZNF217
Homo sapiens CD8-positive, alpha-beta memory T cell modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)
CRISPR knockout RNA-seq integrating a guide (sgRNA) library targeting transcription start sites in ZNF217
Homo sapiens CD8-positive, alpha-beta memory T cell modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)
CRISPR knockout RNA-seq integrating a guide (sgRNA) library targeting transcription start sites in ZNF217
Homo sapiens CD8-positive, alpha-beta memory T cell modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)

AnalysisSet Input File Sets

1 item
Accession
Summary
CRISPR knockout RNA-seq integrating a non-targeting guide (sgRNA) library with non-targeting control