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IGVFDS9078ZWQH

released
Aliases
jay-shendure:analysis_set_CRISPRa_K562
File Set Type
principal analysis
Summary
scCRISPR activation screen: differential gene expression quantifications, filtered feature barcode matrix
Workflows
Publications
Samples
K562 cell line, modified with CRISPR modifications, transfected with a guide library
Protocols

Files

3 items
csv
differential gene expression quantifications
differential gene expression quantifications
Jay Shendure, UW
3.3 KB
validated
csv
differential gene expression quantifications
differential gene expression quantifications
Jay Shendure, UW
6.9 KB
validated
tar
filtered feature barcode matrix
cell by gene in filtered feature barcode matrix
Jay Shendure, UW
538 MB
validated

File Association Graph

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File Set
File
AnalysisSet
AuxiliarySet
MeasurementSet

Samples

4 items
Sample Terms
Associated Construct Library Sets
Summary
InVitroSystem
K562 cell line (cellular sub pool: 10Xlane1), female, Homo sapiens modified with CRISPRa Sp-dCas9-VP64-p65-Rta (VPR), transfected with a guide library
InVitroSystem
K562 cell line (cellular sub pool: 10Xlane1), female, Homo sapiens modified with CRISPRa Sp-dCas9-VP64, transfected with a guide library
InVitroSystem
K562 cell line (cellular sub pool: 10Xlane2), female, Homo sapiens modified with CRISPRa Sp-dCas9-VP64, transfected with a guide library
InVitroSystem
K562 cell line (cellular sub pool: 10Xlane2), female, Homo sapiens modified with CRISPRa Sp-dCas9-VP64-p65-Rta (VPR), transfected with a guide library

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_K562
female
Homo sapiens

Associated Construct Library Sets

1 item
Accession
Summary
Library Design Files
guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci

MeasurementSet Input File Sets

4 items
Associated Auxiliary Sets
scCRISPR activation screen integrating a guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci
K562 cell line (cellular sub pool: 10Xlane2), female, Homo sapiens modified with CRISPRa Sp-dCas9-VP64, transfected with a guide library
scCRISPR activation screen integrating a guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci
K562 cell line (cellular sub pool: 10Xlane1), female, Homo sapiens modified with CRISPRa Sp-dCas9-VP64-p65-Rta (VPR), transfected with a guide library
scCRISPR activation screen integrating a guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci
K562 cell line (cellular sub pool: 10Xlane1), female, Homo sapiens modified with CRISPRa Sp-dCas9-VP64, transfected with a guide library
scCRISPR activation screen integrating a guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci
K562 cell line (cellular sub pool: 10Xlane2), female, Homo sapiens modified with CRISPRa Sp-dCas9-VP64-p65-Rta (VPR), transfected with a guide library

AuxiliarySet Input File Sets

4 items
Associated Measurement Sets
gRNA sequencing for scCRISPR activation screen integrating a guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci
K562 cell line (cellular sub pool: 10Xlane1), female, Homo sapiens modified with CRISPRa Sp-dCas9-VP64-p65-Rta (VPR), transfected with a guide library
gRNA sequencing for scCRISPR activation screen integrating a guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci
K562 cell line (cellular sub pool: 10Xlane2), female, Homo sapiens modified with CRISPRa Sp-dCas9-VP64, transfected with a guide library
gRNA sequencing for scCRISPR activation screen integrating a guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci
K562 cell line (cellular sub pool: 10Xlane2), female, Homo sapiens modified with CRISPRa Sp-dCas9-VP64-p65-Rta (VPR), transfected with a guide library
gRNA sequencing for scCRISPR activation screen integrating a guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci
K562 cell line (cellular sub pool: 10Xlane1), female, Homo sapiens modified with CRISPRa Sp-dCas9-VP64, transfected with a guide library

Attribution

Award
HG011966
Principal Investigator(s)
Contact P.I.
Jay Shendure
Lab
Jay Shendure, UW