{
"@context": "/terms/",
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"FileSet",
"Item"
],
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],
"assay_titles": [
"scCRISPR screen"
],
"audit": {
"WARNING": [
{
"category": "missing analysis step version",
"detail": "Analysis set [IGVFDS9078ZWQH](/analysis-sets/IGVFDS9078ZWQH/) links to file(s) [IGVFFI5652WMRK](/tabular-files/IGVFFI5652WMRK/), [IGVFFI4612KEHH](/tabular-files/IGVFFI4612KEHH/) that are missing `analysis_step_version`. Analysis set files are expected to specify analysis step version.",
"level": 40,
"level_name": "WARNING",
"name": "audit_analysis_set_files_missing_analysis_step_version",
"path": "/analysis-sets/IGVFDS9078ZWQH/"
},
{
"category": "missing derived from",
"detail": "Analysis set [IGVFDS9078ZWQH](/analysis-sets/IGVFDS9078ZWQH/) links to file(s) [IGVFFI5652WMRK](/tabular-files/IGVFFI5652WMRK/), [IGVFFI4612KEHH](/tabular-files/IGVFFI4612KEHH/) that have no `derived_from`. Files in an analysis set are expected to be derived from other files.",
"level": 40,
"level_name": "WARNING",
"name": "audit_input_file_sets_derived_from",
"path": "/analysis-sets/IGVFDS9078ZWQH/"
}
]
},
"award": {
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"component": "functional characterization",
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"title": "Jay Shendure"
},
"title": "Massively parallel characterization of variants and elements impacting transcriptional regulation in dynamic cellular systems"
},
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"creation_timestamp": "2023-12-12T23:07:33.755844+00:00",
"donors": [
{
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"accession": "IGVFDO9208RPQQ",
"aliases": [
"igvf:donor_of_K562"
],
"sex": "female",
"status": "released",
"taxa": "Homo sapiens"
}
],
"file_set_type": "principal analysis",
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"aliases": [
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"creation_timestamp": "2023-12-13T00:09:56.255569+00:00",
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"submitted_file_name": "/net/shendure/vol10/projects/IGVFtemp/Shendure_K562_CRISPRa_matrix.tar.gz",
"upload_status": "validated"
},
{
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"aliases": [
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"content_type": "differential gene expression quantifications",
"creation_timestamp": "2024-10-14T18:38:59.978827+00:00",
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"upload_status": "validated"
},
{
"@id": "/tabular-files/IGVFFI4612KEHH/",
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],
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"creation_timestamp": "2024-10-14T18:39:03.562166+00:00",
"file_format": "csv",
"file_size": 3314,
"href": "/tabular-files/IGVFFI4612KEHH/@@download/IGVFFI4612KEHH.csv.gz",
"s3_uri": "s3://igvf-files/2024/10/14/12aa9f23-4832-4e9e-9cc2-9d94f4545063/IGVFFI4612KEHH.csv.gz",
"submitted_file_name": "/net/shendure/vol1/home/martin91/IGVF/forupload/CRISPRa/41467_2024_52490_MOESM14_ESM.csv.gz",
"upload_status": "validated"
}
],
"functional_assay_mechanisms": [],
"input_file_sets": [
{
"@id": "/measurement-sets/IGVFDS4863BMGZ/",
"accession": "IGVFDS4863BMGZ",
"aliases": [
"jay-shendure:CRISPRa_singlecell_K562_VP64_10Xlane1_exprs"
],
"file_set_type": "experimental data"
},
{
"@id": "/measurement-sets/IGVFDS8535CXVX/",
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"jay-shendure:CRISPRa_singlecell_K562_VP64_10Xlane2_exprs"
],
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},
{
"@id": "/measurement-sets/IGVFDS7395DPAD/",
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],
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},
{
"@id": "/measurement-sets/IGVFDS0508ERUQ/",
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],
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},
{
"@id": "/auxiliary-sets/IGVFDS4502LQQH/",
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"aliases": [
"jay-shendure:CRISPRa_singlecell_K562_VP64_10Xlane1_CS1"
],
"file_set_type": "gRNA sequencing"
},
{
"@id": "/auxiliary-sets/IGVFDS8929VNCP/",
"accession": "IGVFDS8929VNCP",
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"jay-shendure:CRISPRa_singlecell_K562_VP64_10Xlane2_CS1"
],
"file_set_type": "gRNA sequencing"
},
{
"@id": "/auxiliary-sets/IGVFDS4621HUKP/",
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"aliases": [
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],
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},
{
"@id": "/auxiliary-sets/IGVFDS4834SKZS/",
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"aliases": [
"jay-shendure:CRISPRa_singlecell_K562_VPR_10Xlane2_CS1"
],
"file_set_type": "gRNA sequencing"
}
],
"input_for": [],
"lab": {
"@id": "/labs/jay-shendure/",
"title": "Jay Shendure, UW"
},
"notes": "This object's file_set_type was primary analysis and has been updated to be principal analysis.",
"protocols": [
"https://www.protocols.io/view/methods-from-multiplex-single-cell-crispra-screeni-c5zvy766"
],
"publications": [
{
"@id": "/publications/20fcbdbb-5a47-41c6-b80b-e6dd0e8a8d3e/",
"publication_identifiers": [
"doi:10.1101/2023.03.28.534017"
]
}
],
"release_timestamp": "2024-08-13T20:47:06.196314+00:00",
"sample_summary": "K562 cell line, modified with CRISPR modifications, transfected with a guide library",
"samples": [
{
"@id": "/in-vitro-systems/IGVFSM1594ZOQH/",
"@type": [
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"Sample",
"Item"
],
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],
"construct_library_sets": [
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"modifications": [
{
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"modality": "activation"
}
],
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{
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"Item"
],
"aliases": [
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],
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"term_name": "K562"
}
],
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"summary": "K562 cell line (cellular sub pool: 10Xlane1), female, Homo sapiens modified with CRISPRa Sp-dCas9-VP64-p65-Rta (VPR), transfected with a guide library",
"taxa": "Homo sapiens"
},
{
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"Sample",
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],
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],
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"modality": "activation"
}
],
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{
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"Item"
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],
"status": "released",
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"term_name": "K562"
}
],
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"summary": "K562 cell line (cellular sub pool: 10Xlane2), female, Homo sapiens modified with CRISPRa Sp-dCas9-VP64, transfected with a guide library",
"taxa": "Homo sapiens"
},
{
"@id": "/in-vitro-systems/IGVFSM9542NUGS/",
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"Biosample",
"Sample",
"Item"
],
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],
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}
],
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"summary": "K562 cell line (cellular sub pool: 10Xlane2), female, Homo sapiens modified with CRISPRa Sp-dCas9-VP64-p65-Rta (VPR), transfected with a guide library",
"taxa": "Homo sapiens"
},
{
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"Biosample",
"Sample",
"Item"
],
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],
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],
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{
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],
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{
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"Item"
],
"aliases": [
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],
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}
],
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"summary": "K562 cell line (cellular sub pool: 10Xlane1), female, Homo sapiens modified with CRISPRa Sp-dCas9-VP64, transfected with a guide library",
"taxa": "Homo sapiens"
}
],
"schema_version": "9",
"status": "released",
"submitted_by": {
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"title": "Beth Martin"
},
"submitted_files_timestamp": "2023-12-13T00:09:56.255569+00:00",
"summary": "scCRISPR activation screen: differential gene expression quantifications, sparse gene count matrix",
"uuid": "5257dd49-492f-49b1-88fd-8b812ae8c188",
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{
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"name": "Cell Ranger Gene Count Pipeline"
}
]
}