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INDEXED
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AnalysisSet
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IGVFDS6924DJJC
IGVFDS6924DJJC
released
Json view
Aliases
charles-gersbach:cd8-tcells-intermediate-analysis-rna-seq-cas-ko-nt
File Set Type
intermediate analysis
Summary
knockout RNA-seq: transcriptome alignments
Workflows
Gersbach GGR RNA-seq Pipeline (IGVFWF6270QWBB)
Publications
Transcriptional and epigenetic regulators of human CD8+ T cell function identified through orthogonal CRISPR screens
Samples
CD8-positive, alpha-beta memory T cell primary cell, modified with CRISPR modifications, transfected with a guide library
Protocols
https://www.protocols.io/view/tfome-crisprko-screens-81wgbxk8olpk/v1
https://www.protocols.io/view/rnaseq-of-primary-human-t-cells-overexpressing-bat-c5pdy5i6
Files
3 items
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
IGVFFI0753LZLZ
bam
transcriptome alignments
GRCh38 filtered transcriptome alignments
Charles Gersbach, Duke
2.7 GB
validated
IGVFFI1477HUUT
bam
transcriptome alignments
GRCh38 filtered transcriptome alignments
Charles Gersbach, Duke
2.3 GB
validated
IGVFFI9520OPSC
bam
transcriptome alignments
GRCh38 filtered transcriptome alignments
Charles Gersbach, Duke
2.5 GB
validated
File Association Graph
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File Set
File
AnalysisSet
MeasurementSet
Samples
3 items
Accession
Type
Sample Terms
Associated Construct Library Sets
Summary
IGVFSM0383IKGE
PrimaryCell
CD8-positive, alpha-beta memory T cell
IGVFDS9830PJVQ
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)
IGVFSM1498WFCO
PrimaryCell
CD8-positive, alpha-beta memory T cell
IGVFDS9830PJVQ
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)
IGVFSM6537MPGY
PrimaryCell
CD8-positive, alpha-beta memory T cell
IGVFDS9830PJVQ
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)
Donors
3 items
Accession
Aliases
Sex
Taxa
IGVFDO4048LCOU
charles-gersbach:cd8-tcells-donor-14
unspecified
Homo sapiens
IGVFDO7589UJKG
charles-gersbach:cd8-tcells-donor-13
unspecified
Homo sapiens
IGVFDO8565PQXZ
charles-gersbach:cd8-tcells-donor-12
unspecified
Homo sapiens
Associated Construct Library Sets
1 item
Accession
Summary
Library Design Files
IGVFDS9830PJVQ
non-targeting guide (sgRNA) library
IGVFFI4223LNAY
MeasurementSet Input File Sets
Show aliases columns
3 items
Measurement Sets
Measured Samples
IGVFDS9846EQXV
CRISPR knockout RNA-seq integrating a non-targeting guide (sgRNA) library
IGVFSM0383IKGE
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)
IGVFDS6088VPGO
CRISPR knockout RNA-seq integrating a non-targeting guide (sgRNA) library
IGVFSM1498WFCO
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)
IGVFDS4169BMPH
CRISPR knockout RNA-seq integrating a non-targeting guide (sgRNA) library
IGVFSM6537MPGY
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)
File Sets Using This Analysis Set as an Input
2 items
Accession
Type
Summary
Aliases
Lab
IGVFDS7239LNZO
AnalysisSet
knockout RNA-seq: fold change over control, transcript quantifications, transcriptome alignments
charles-gersbach:cd8-tcells-analysis-set-rna-seq-cas-ko-gata3
Charles Gersbach, Duke
IGVFDS9906UGWB
AnalysisSet
knockout RNA-seq: fold change over control, transcript quantifications, transcriptome alignments
charles-gersbach:cd8-tcells-analysis-set-rna-seq-cas-ko-znf217
Charles Gersbach, Duke
Attribution
Award
HG012053
Principal Investigator(s)
Charles Gersbach
,
Gregory Crawford
,
Tim Reddy
Contact P.I.
Charles Gersbach
Lab
Charles Gersbach, Duke