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IGVFDS0021NCLH

released
Aliases
richard-sherwood:18loci_ABE_LDLuptake_bean_analysis_set
File Set Type
principal analysis
Summary
CRISPR base editing FACS screen: guide quantifications, variant effects
Workflows
Samples
HepG2 cell line, modified with CRISPR modifications, transfected with a guide library, sorted into bins
Protocols

Files

3 items
csv
variant effects
variant effects
Richard Sherwood, Brigham and Women's Hospital
128 KB
validated
csv
guide quantifications
guide quantifications
Richard Sherwood, Brigham and Women's Hospital
185 KB
validated
csv
guide quantifications
guide quantifications
Richard Sherwood, Brigham and Women's Hospital
336 KB
validated

File Association Graph

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File Set
File
AnalysisSet
MeasurementSet

Samples

20 items
Sample Terms
Associated Construct Library Sets
Summary
InVitroSystem
HepG2 cell line, male, Homo sapiens (sorting details: Sorted for Bottom 20% LDL-C uptake) modified with CRISPR base editing SpRY-ABE8e, transduced (lentivirus) with a guide library (MOI of 0.5)
InVitroSystem
HepG2 cell line, male, Homo sapiens (sorting details: Sorted for Bottom 20-40% LDL-C uptake) modified with CRISPR base editing SpRY-ABE8e, transduced (lentivirus) with a guide library (MOI of 0.5)
InVitroSystem
HepG2 cell line, male, Homo sapiens (sorting details: Sorted for Top 20% LDL-C uptake) modified with CRISPR base editing SpRY-ABE8e, transduced (lentivirus) with a guide library (MOI of 0.5)
InVitroSystem
HepG2 cell line, male, Homo sapiens (sorting details: Sorted for Bottom 20-40% LDL-C uptake) modified with CRISPR base editing SpRY-ABE8e, transduced (lentivirus) with a guide library (MOI of 0.5)
InVitroSystem
HepG2 cell line, male, Homo sapiens (sorting details: Sorted for Bottom 20% LDL-C uptake) modified with CRISPR base editing SpRY-ABE8e, transduced (lentivirus) with a guide library (MOI of 0.5)
InVitroSystem
HepG2 cell line, male, Homo sapiens modified with CRISPR base editing SpRY-ABE8e, transduced (lentivirus) with a guide library (MOI of 0.5)
InVitroSystem
HepG2 cell line, male, Homo sapiens modified with CRISPR base editing SpRY-ABE8e, transduced (lentivirus) with a guide library (MOI of 0.5)
InVitroSystem
HepG2 cell line, male, Homo sapiens (sorting details: Sorted for Top 20-40% LDL-C uptake) modified with CRISPR base editing SpRY-ABE8e, transduced (lentivirus) with a guide library (MOI of 0.5)
InVitroSystem
HepG2 cell line, male, Homo sapiens (sorting details: Sorted for Top 20-40% LDL-C uptake) modified with CRISPR base editing SpRY-ABE8e, transduced (lentivirus) with a guide library (MOI of 0.5)
InVitroSystem
HepG2 cell line, male, Homo sapiens (sorting details: Sorted for Top 20-40% LDL-C uptake) modified with CRISPR base editing SpRY-ABE8e, transduced (lentivirus) with a guide library (MOI of 0.5)

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_HepG2
male
Homo sapiens

Associated Construct Library Sets

1 item
Accession
Summary
Library Design Files
guide (sgRNA) library targeting phenotype-associated variants genome-wide

MeasurementSet Input File Sets

20 items
CRISPR base editing FACS screen integrating a guide (sgRNA) library targeting phenotype-associated variants genome-wide
HepG2 cell line, male, Homo sapiens (sorting details: Sorted for Bottom 20% LDL-C uptake) modified with CRISPR base editing SpRY-ABE8e, transduced (lentivirus) with a guide library (MOI of 0.5)
CRISPR base editing FACS screen integrating a guide (sgRNA) library targeting phenotype-associated variants genome-wide
HepG2 cell line, male, Homo sapiens (sorting details: Sorted for Top 20% LDL-C uptake) modified with CRISPR base editing SpRY-ABE8e, transduced (lentivirus) with a guide library (MOI of 0.5)
CRISPR base editing FACS screen integrating a guide (sgRNA) library targeting phenotype-associated variants genome-wide
HepG2 cell line, male, Homo sapiens modified with CRISPR base editing SpRY-ABE8e, transduced (lentivirus) with a guide library (MOI of 0.5)
CRISPR base editing FACS screen integrating a guide (sgRNA) library targeting phenotype-associated variants genome-wide
HepG2 cell line, male, Homo sapiens (sorting details: Sorted for Top 20-40% LDL-C uptake) modified with CRISPR base editing SpRY-ABE8e, transduced (lentivirus) with a guide library (MOI of 0.5)
CRISPR base editing FACS screen integrating a guide (sgRNA) library targeting phenotype-associated variants genome-wide
HepG2 cell line, male, Homo sapiens (sorting details: Sorted for Bottom 20-40% LDL-C uptake) modified with CRISPR base editing SpRY-ABE8e, transduced (lentivirus) with a guide library (MOI of 0.5)
CRISPR base editing FACS screen integrating a guide (sgRNA) library targeting phenotype-associated variants genome-wide
HepG2 cell line, male, Homo sapiens (sorting details: Sorted for Bottom 20% LDL-C uptake) modified with CRISPR base editing SpRY-ABE8e, transduced (lentivirus) with a guide library (MOI of 0.5)
CRISPR base editing FACS screen integrating a guide (sgRNA) library targeting phenotype-associated variants genome-wide
HepG2 cell line, male, Homo sapiens (sorting details: Sorted for Top 20-40% LDL-C uptake) modified with CRISPR base editing SpRY-ABE8e, transduced (lentivirus) with a guide library (MOI of 0.5)
CRISPR base editing FACS screen integrating a guide (sgRNA) library targeting phenotype-associated variants genome-wide
HepG2 cell line, male, Homo sapiens modified with CRISPR base editing SpRY-ABE8e, transduced (lentivirus) with a guide library (MOI of 0.5)
CRISPR base editing FACS screen integrating a guide (sgRNA) library targeting phenotype-associated variants genome-wide
HepG2 cell line, male, Homo sapiens modified with CRISPR base editing SpRY-ABE8e, transduced (lentivirus) with a guide library (MOI of 0.5)
CRISPR base editing FACS screen integrating a guide (sgRNA) library targeting phenotype-associated variants genome-wide
HepG2 cell line, male, Homo sapiens (sorting details: Sorted for Top 20-40% LDL-C uptake) modified with CRISPR base editing SpRY-ABE8e, transduced (lentivirus) with a guide library (MOI of 0.5)

Attribution

Award
HG012010
Principal Investigator(s)
Contact P.I.
Luca Pinello
Lab
Richard Sherwood, Brigham and Women's Hospital