IGVFFI9930ZUUB

released
validated
{
    "@context": "/terms/",
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    "accession": "IGVFFI9930ZUUB",
    "aliases": [
        "charles-gersbach:cd8-tcells-guide-counts-crispr-ko-tfome"
    ],
    "analysis_step_version": "/analysis-step-versions/886b365e-3105-424a-8972-7476238b54d7/",
    "assay_titles": [
        "CRISPR FACS screen"
    ],
    "assembly": "custom",
    "audit": {},
    "award": {
        "@id": "/awards/HG012053/",
        "component": "functional characterization"
    },
    "barcode_map_for": [],
    "content_md5sum": "fdcc12df11e04b90f7d30d842e4f5cbd",
    "content_type": "guide quantifications",
    "controlled_access": false,
    "creation_timestamp": "2024-09-20T18:39:36.212145+00:00",
    "derived_from": [
        "/alignment-files/IGVFFI6153GXKL/",
        "/alignment-files/IGVFFI4092OXMN/",
        "/alignment-files/IGVFFI9097APHN/",
        "/alignment-files/IGVFFI4927HZXQ/"
    ],
    "file_format": "csv",
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    "file_set": {
        "@id": "/analysis-sets/IGVFDS5106XTZH/",
        "accession": "IGVFDS5106XTZH",
        "file_set_type": "principal analysis",
        "samples": [
            {
                "@id": "/primary-cells/IGVFSM2624KTRK/",
                "accession": "IGVFSM2624KTRK",
                "classifications": [
                    "primary cell"
                ],
                "modifications": [
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                        "modality": "knockout",
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                        "summary": "CRISPRko Sp-Cas9"
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                ],
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                        "@id": "/sample-terms/CL_0000909/",
                        "status": "released",
                        "term_name": "CD8-positive, alpha-beta memory T cell"
                    }
                ],
                "status": "released",
                "summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL7R) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)",
                "taxa": "Homo sapiens"
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            {
                "@id": "/primary-cells/IGVFSM7226CVDS/",
                "accession": "IGVFSM7226CVDS",
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                        "summary": "CRISPRko Sp-Cas9"
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                        "status": "released",
                        "term_name": "CD8-positive, alpha-beta memory T cell"
                    }
                ],
                "status": "released",
                "summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL7R) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)",
                "taxa": "Homo sapiens"
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            {
                "@id": "/primary-cells/IGVFSM4936HKEW/",
                "accession": "IGVFSM4936HKEW",
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                        "@id": "/crispr-modifications/9fef21f2-9727-4a97-a5a7-c590a637c101/",
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                        "status": "released",
                        "term_name": "CD8-positive, alpha-beta memory T cell"
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                ],
                "status": "released",
                "summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL7R) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)",
                "taxa": "Homo sapiens"
            },
            {
                "@id": "/primary-cells/IGVFSM0466RHGQ/",
                "accession": "IGVFSM0466RHGQ",
                "classifications": [
                    "primary cell"
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                "modifications": [
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                        "@id": "/crispr-modifications/9fef21f2-9727-4a97-a5a7-c590a637c101/",
                        "modality": "knockout",
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                        "summary": "CRISPRko Sp-Cas9"
                    }
                ],
                "sample_terms": [
                    {
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                        "status": "released",
                        "term_name": "CD8-positive, alpha-beta memory T cell"
                    }
                ],
                "status": "released",
                "summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL7R) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)",
                "taxa": "Homo sapiens"
            }
        ],
        "status": "released",
        "summary": "CRISPR knockout FACS screen targeting IL7R: fold change over control, guide quantifications"
    },
    "file_size": 146689,
    "gene_list_for": [],
    "href": "/tabular-files/IGVFFI9930ZUUB/@@download/IGVFFI9930ZUUB.csv.gz",
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    "integrated_in": [],
    "lab": {
        "@id": "/labs/charles-gersbach/",
        "title": "Charles Gersbach, Duke"
    },
    "loci_list_for": [],
    "md5sum": "5476df28b42297a12be0de03b764c966",
    "release_timestamp": "2024-11-18T21:09:11.343236+00:00",
    "s3_uri": "s3://igvf-files/2024/09/20/3d775eae-5d57-47a2-bb4c-f58876ed6e2a/IGVFFI9930ZUUB.tsv.gz",
    "schema_version": "13",
    "status": "released",
    "submitted_by": {
        "@id": "/users/29f5f78d-ac90-421e-90c6-a90e1dc407a7/",
        "title": "Alejandro Barrera"
    },
    "submitted_file_name": "/data/reddylab/Alex/IGVF/data_submissions/processed_data/AIM_2_T_cell_project_1/CRISPR.TFome.ko.without.BATF3.counts.table.csv.gz",
    "summary": "custom assembly guide quantifications",
    "upload_status": "validated",
    "uuid": "3d775eae-5d57-47a2-bb4c-f58876ed6e2a"
}