{
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"aliases": [
"jesse-engreitz:auxiliary_sortparams_file_CRUDO_MYC-Auxin6hrs-FF2"
],
"assay_titles": [
"CRISPR FlowFISH screen"
],
"audit": {
"NOT_COMPLIANT": [
{
"category": "missing file format specifications",
"detail": "Tabular file [IGVFFI9629PGSJ](/tabular-files/IGVFFI9629PGSJ/) has no `file_format_specifications`. Tabular files are expected to link to a file format specifications document describing the headers of the file.",
"level": 50,
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}
]
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"award": {
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"component": "functional characterization"
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"content_md5sum": "10cae2d09ad9d0cd73de32bb1bfee8da",
"content_type": "sample sort parameters",
"controlled_access": false,
"creation_timestamp": "2024-07-03T00:35:59.089566+00:00",
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"accession": "IGVFDS5394TNHK",
"file_set_type": "cell sorting",
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{
"@id": "/in-vitro-systems/IGVFSM9689GGGO/",
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{
"@id": "/degron-modifications/95081d1b-08b4-49ed-b324-e53fc43d8456/",
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"summary": "AID system targeting RAD21"
},
{
"@id": "/crispr-modifications/8543fa06-1720-4e7d-8e8f-7e24c1b1cf79/",
"modality": "interference",
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"summary": "CRISPRi Sp-dCas9-KOX1-KRAB"
}
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"summary": "HCT116 cell line, male, Homo sapiens (sorting details: FACS bin A for 0-10% expression of MYC) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library",
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{
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"purpose": "activation",
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"summary": "Treatment of 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours",
"treatment_term_name": "5-Phenyl-1H-indole-3-acetic acid"
}
]
},
{
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{
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"summary": "AID system targeting RAD21"
},
{
"@id": "/crispr-modifications/8543fa06-1720-4e7d-8e8f-7e24c1b1cf79/",
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"taxa": "Homo sapiens",
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{
"@id": "/treatments/d35868d1-c45b-49ae-8ff3-a9c0c22551c2/",
"purpose": "activation",
"status": "released",
"summary": "Treatment of 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours",
"treatment_term_name": "5-Phenyl-1H-indole-3-acetic acid"
}
]
},
{
"@id": "/in-vitro-systems/IGVFSM8399ZECZ/",
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{
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},
{
"@id": "/crispr-modifications/8543fa06-1720-4e7d-8e8f-7e24c1b1cf79/",
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}
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"status": "released",
"term_name": "HCT116"
}
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{
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"purpose": "activation",
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"treatment_term_name": "5-Phenyl-1H-indole-3-acetic acid"
}
]
},
{
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{
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"summary": "AID system targeting RAD21"
},
{
"@id": "/crispr-modifications/8543fa06-1720-4e7d-8e8f-7e24c1b1cf79/",
"modality": "interference",
"status": "released",
"summary": "CRISPRi Sp-dCas9-KOX1-KRAB"
}
],
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{
"@id": "/sample-terms/EFO_0002824/",
"status": "released",
"term_name": "HCT116"
}
],
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{
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"purpose": "activation",
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"treatment_term_name": "5-Phenyl-1H-indole-3-acetic acid"
}
]
},
{
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],
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{
"@id": "/degron-modifications/95081d1b-08b4-49ed-b324-e53fc43d8456/",
"modality": "degradation",
"status": "released",
"summary": "AID system targeting RAD21"
},
{
"@id": "/crispr-modifications/8543fa06-1720-4e7d-8e8f-7e24c1b1cf79/",
"modality": "interference",
"status": "released",
"summary": "CRISPRi Sp-dCas9-KOX1-KRAB"
}
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"status": "released",
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}
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{
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"purpose": "activation",
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"summary": "Treatment of 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours",
"treatment_term_name": "5-Phenyl-1H-indole-3-acetic acid"
}
]
},
{
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},
{
"@id": "/crispr-modifications/8543fa06-1720-4e7d-8e8f-7e24c1b1cf79/",
"modality": "interference",
"status": "released",
"summary": "CRISPRi Sp-dCas9-KOX1-KRAB"
}
],
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}
],
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{
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"purpose": "activation",
"status": "released",
"summary": "Treatment of 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours",
"treatment_term_name": "5-Phenyl-1H-indole-3-acetic acid"
}
]
}
],
"status": "released",
"summary": "cell sorting for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, ... and 4 more measurement sets"
},
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"lab": {
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"title": "Jesse Engreitz, Stanford"
},
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},
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}