IGVFFI9150SKWI

1{
2    "@context": "/terms/",
3    "@id": "/tabular-files/IGVFFI9150SKWI/",
4    "@type": [
5        "TabularFile",
6        "File",
7        "Item"
8    ],
9    "accession": "IGVFFI9150SKWI",
10    "aliases": [
11        "charles-gersbach:cd8-tcell-ko-gata3-grnas-metadata-v5"
12    ],
13    "assay_titles": [],
14    "assembly": "GRCh38",
15    "audit": {
16        "INTERNAL_ACTION": [
17            {
18                "category": "mismatched status",
19                "detail": "Released tabular file [IGVFFI9150SKWI](/tabular-files/IGVFFI9150SKWI/) has in progress subobject curated set [IGVFDS0876APCC](/curated-sets/IGVFDS0876APCC/).",
20                "level": 30,
21                "level_name": "INTERNAL_ACTION",
22                "name": "audit_item_status",
23                "path": "/tabular-files/IGVFFI9150SKWI/"
24            }
25        ]
26    },
27    "award": {
28        "@id": "/awards/HG012053/",
29        "component": "functional characterization"
30    },
31    "barcode_map_for": [],
32    "checkfiles_version": "v20",
33    "content_md5sum": "241b01d6b96124018be489fb1e3d950b",
34    "content_type": "guide RNA sequences",
35    "controlled_access": false,
36    "creation_timestamp": "2025-03-21T03:18:01.886679+00:00",
37    "file_format": "tsv",
38    "file_format_specifications": [
39        {
40            "@id": "/documents/78670549-3719-42bc-8df2-92d98918fa27/",
41            "description": "File format specification for per-guide metadata used in a CRISPR experiment",
42            "standardized_file_format": true
43        }
44    ],
45    "file_set": {
46        "@id": "/curated-sets/IGVFDS0876APCC/",
47        "@type": [
48            "CuratedSet",
49            "FileSet",
50            "Item"
51        ],
52        "accession": "IGVFDS0876APCC",
53        "file_set_type": "guide RNAs",
54        "status": "in progress",
55        "summary": "Homo sapiens GRCh38 guide RNAs",
56        "taxa": "Homo sapiens"
57    },
58    "file_size": 249,
59    "gene_list_for": [],
60    "href": "/tabular-files/IGVFFI9150SKWI/@@download/IGVFFI9150SKWI.tsv.gz",
61    "input_file_for": [],
62    "integrated_in": [
63        {
64            "@id": "/construct-library-sets/IGVFDS2398ZXBQ/",
65            "applied_to_samples": [
66                {
67                    "@id": "/primary-cells/IGVFSM9627TJZO/",
68                    "sample_terms": [
69                        {
70                            "@id": "/sample-terms/CL_0000909/",
71                            "summary": "CD8-positive, alpha-beta memory T cell",
72                            "term_name": "CD8-positive, alpha-beta memory T cell"
73                        }
74                    ],
75                    "summary": "Homo sapiens CD8-positive, alpha-beta memory T cell modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)"
76                },
77                {
78                    "@id": "/primary-cells/IGVFSM7117ATUZ/",
79                    "sample_terms": [
80                        {
81                            "@id": "/sample-terms/CL_0000909/",
82                            "summary": "CD8-positive, alpha-beta memory T cell",
83                            "term_name": "CD8-positive, alpha-beta memory T cell"
84                        }
85                    ],
86                    "summary": "Homo sapiens CD8-positive, alpha-beta memory T cell modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)"
87                },
88                {
89                    "@id": "/primary-cells/IGVFSM0829UPAR/",
90                    "sample_terms": [
91                        {
92                            "@id": "/sample-terms/CL_0000909/",
93                            "summary": "CD8-positive, alpha-beta memory T cell",
94                            "term_name": "CD8-positive, alpha-beta memory T cell"
95                        }
96                    ],
97                    "summary": "Homo sapiens CD8-positive, alpha-beta memory T cell modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)"
98                }
99            ],
100            "file_set_type": "guide library",
101            "small_scale_gene_list": [
102                {
103                    "@id": "/genes/ENSG00000107485/",
104                    "status": "released",
105                    "symbol": "GATA3"
106                }
107            ],
108            "status": "released",
109            "summary": "guide (sgRNA) library targeting transcription start sites in GATA3"
110        }
111    ],
112    "lab": {
113        "@id": "/labs/charles-gersbach/",
114        "title": "Charles Gersbach, Duke"
115    },
116    "loci_list_for": [],
117    "md5sum": "de23db9f4f6f47509985d63fd649883a",
118    "primer_design_for": [],
119    "quality_metrics": [],
120    "release_timestamp": "2025-04-25T03:34:38.493652+00:00",
121    "s3_uri": "s3://igvf-files/2025/03/21/15a01c06-1866-46e5-a3f8-cc3606bb35e5/IGVFFI9150SKWI.tsv.gz",
122    "schema_version": "15",
123    "status": "released",
124    "submitted_by": {
125        "@id": "/users/29f5f78d-ac90-421e-90c6-a90e1dc407a7/",
126        "title": "Alejandro Barrera"
127    },
128    "submitted_file_name": "/data/reddylab/Alex/IGVF/data_submissions/reference_data/AIM_2_T_cell_project_1/cas9_ko.GATA3.igvf_metadata.v6.txt.gz",
129    "summary": "GRCh38 guide RNA sequences",
130    "upload_status": "validated",
131    "uuid": "15a01c06-1866-46e5-a3f8-cc3606bb35e5"
132}