IGVFFI7903UEWZ

preview
validated
This object is in preview status. Metadata is subject to change until this object is released.
File Set
IGVFDS1863TJOE(CRISPR prime editing Variant-EFFECTS targeting PPIF integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)
Summary
hg19 variant effects
Analysis Step Version
File Format
tsv
Content Type
variant effects
Aliases
jesse-engreitz:PPIF_promoter_8mer_insertion_Jurkat-Stimulation_Variant-FlowFISH_variant_effects_file
md5sum
fb335b519c0adb667975ce578b7eacaa
Content MD5sum
b2e23517658c341a3744d39f513f62a5
File Size
2.7 KB
Submitted File Name
PPIF_promoter_8mer_insertion_Jurkat-Stimulation_AllelicEffectsStats.tsv.gz
Checkfiles Version
Attribution

Reference Source Details

Genome Assembly
hg19

File Format Specifications

1 item
Page
Description
Type
Download
Standardized File Format
Preview
explanation of columns in analysis summary file output by the Variant-EFFECTS pipeline
file format specification

Samples

56 items
Sample Terms
Summary
InVitroSystem
Homo sapiens (male) Jurkat cell line (sorting details: FACS bin for 0-10% expression of PPIF) treated with 100 ng/mL phorbol 13-acetate 12-myristate for 4 hours, 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 4 hours, modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transduced (lentivirus) with a guide library
InVitroSystem
Homo sapiens (male) Jurkat cell line treated with 100 ng/mL phorbol 13-acetate 12-myristate for 4 hours, 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 4 hours, modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transduced (lentivirus) with a guide library
InVitroSystem
Homo sapiens (male) Jurkat cell line (sorting details: FACS bin for 90-100% expression of PPIF) treated with 100 ng/mL phorbol 13-acetate 12-myristate for 4 hours, 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 4 hours, modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transduced (lentivirus) with a guide library
InVitroSystem
Homo sapiens (male) Jurkat cell line (sorting details: FACS bin for 80-90% expression of PPIF) treated with 100 ng/mL phorbol 13-acetate 12-myristate for 4 hours, 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 4 hours, modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transduced (lentivirus) with a guide library
InVitroSystem
Homo sapiens (male) Jurkat cell line (sorting details: FACS bin for 90-100% expression of PPIF) treated with 100 ng/mL phorbol 13-acetate 12-myristate for 4 hours, 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 4 hours, modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transduced (lentivirus) with a guide library
InVitroSystem
Homo sapiens (male) Jurkat cell line (sorting details: FACS bin for 90-100% expression of PPIF) treated with 100 ng/mL phorbol 13-acetate 12-myristate for 4 hours, 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 4 hours, modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transduced (lentivirus) with a guide library
InVitroSystem
Homo sapiens (male) Jurkat cell line (sorting details: FACS bin for 11-20% expression of PPIF) treated with 100 ng/mL phorbol 13-acetate 12-myristate for 4 hours, 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 4 hours, modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transduced (lentivirus) with a guide library
InVitroSystem
Homo sapiens (male) Jurkat cell line (sorting details: FACS bin for 55-65% expression of PPIF) treated with 100 ng/mL phorbol 13-acetate 12-myristate for 4 hours, 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 4 hours, modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transduced (lentivirus) with a guide library
InVitroSystem
Homo sapiens (male) Jurkat cell line (sorting details: FACS bin for 0-10% expression of PPIF) treated with 100 ng/mL phorbol 13-acetate 12-myristate for 4 hours, 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 4 hours, modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transduced (lentivirus) with a guide library
InVitroSystem
Homo sapiens (male) Jurkat cell line treated with 100 ng/mL phorbol 13-acetate 12-myristate for 4 hours, 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 4 hours, modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transduced (lentivirus) with a guide library

Files This File Derives From

448 items
fastq
reads
Jesse Engreitz, Stanford
8.0 MB
validated
fastq
reads
Jesse Engreitz, Stanford
7.0 MB
validated
fastq
reads
Jesse Engreitz, Stanford
13 MB
validated
fastq
reads
Jesse Engreitz, Stanford
3.2 MB
validated
fastq
reads
Jesse Engreitz, Stanford
3.1 MB
validated
fastq
reads
Jesse Engreitz, Stanford
3.2 MB
validated
fastq
reads
Jesse Engreitz, Stanford
14 MB
validated
fastq
reads
Jesse Engreitz, Stanford
3.2 MB
validated
fastq
reads
Jesse Engreitz, Stanford
3.1 MB
validated
fastq
reads
Jesse Engreitz, Stanford
7.5 MB
validated

Files Derived From This File

1 item
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
tsv
variant effects
GRCh38 variant effects
Jesse Engreitz, Stanford
2.6 KB
validated