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IGVFFI6308XVUC

released
validated
File Set
IGVFDS3913KUFQ(Homo sapiens GRCh38 custom guide RNAs)
Summary
GRCh38 guide RNA sequences
File Format
tsv
Content Type
guide RNA sequences
Aliases
charles-gersbach:cd8-tcell-ccr7-grnas-metadata
md5sum
b8fafbbdb8c781ca6744b0a0b6dabfbd
Content MD5sum
937a494cf4425f1754810c190097cf1a
File Size
46 KB
Submitted File Name
/data/reddylab/Alex/IGVF/data_submissions/reference_data/AIM_2_T_cell_project_1/Final_gRNA_Library.scRNAseq_screen.igvf_metadata.v3.txt.gz
Checkfiles Version
v14

Reference Source Details

Genome Assembly
GRCh38

Samples

15 items
Sample Terms
Summary
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of CCR7) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of CCR7) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of CCR7) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of CCR7) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of CCR7) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of CCR7) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)

Integrated In

1 item
Accession
Type
Summary
Aliases
Lab
ConstructLibrarySet
guide (sgRNA) library targeting transcription start sites in many genes
  • charles-gersbach:cd8-tcell-ccr7-grna-construct-lib-set
Charles Gersbach, Duke

Attribution

Award
HG012053
Principal Investigator(s)
Contact P.I.
Charles Gersbach
Lab
Charles Gersbach, Duke