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IGVFFI6212XMWZ

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validated
This object is in preview status. Metadata is subject to change until this object is released.
File Set
IGVFDS4833ZRTZ(prime editing Variant-EFFECTS targeting PPIF: variant effects)
Summary
variant effects
File Format
tsv
Content Type
variant effects
Aliases
jesse-engreitz:PPIF_enhancer_10pegRNA_validation-THP1_Variant-EFFECTS_variant_effects_file
md5sum
eebe2a1fd7addf7a1c04c51b04b94b17
Content MD5sum
38cd97b42d6be4e7d9396c75844e8820
File Size
916 B
Submitted File Name
PPIF_enhancer_10pegRNA_validation_THP1_AllelicEffectsStats.tsv.gz
Checkfiles Version
Legacy version of checkfiles
Submitter Comment
Output from the Variant-EFFECTS (formerly Variant-FlowFISH) Snakemake pipeline, analyzing the effect of 10 PPIF enhancer variants on PPIF expression in a low MOI validation screen conducted in THP-1 cells

File Format Specifications

1 item
Page
Description
Type
Download
Preview
explanation of columns in analysis summary file output by the Variant-EFFECTS pipeline
file format specification

Samples

41 items
Sample Terms
Summary
InVitroSystem
THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 26-50% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library
InVitroSystem
THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 51-75% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library
InVitroSystem
THP-1 cell line, male, Homo sapiens modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library
InVitroSystem
THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 51-75% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library
InVitroSystem
THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 76-100% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library
InVitroSystem
THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 51-75% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library
InVitroSystem
THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 51-75% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library
InVitroSystem
THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 76-100% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library
InVitroSystem
THP-1 cell line, male, Homo sapiens modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library
InVitroSystem
THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 76-100% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

Files This File Derives From

1608 items
fastq
reads
Jesse Engreitz, Stanford
18 MB
validated
fastq
reads
Jesse Engreitz, Stanford
23 MB
validated
fastq
reads
Jesse Engreitz, Stanford
15 MB
validated
fastq
reads
Jesse Engreitz, Stanford
14 MB
validated
fastq
reads
Jesse Engreitz, Stanford
6.6 MB
validated
fastq
reads
Jesse Engreitz, Stanford
7.1 MB
validated
fastq
reads
Jesse Engreitz, Stanford
16 MB
validated
fastq
reads
Jesse Engreitz, Stanford
17 MB
validated
fastq
reads
Jesse Engreitz, Stanford
12 MB
validated
fastq
reads
Jesse Engreitz, Stanford
6.9 MB
validated

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford