IGVFFI5502YFXX

released

validated

File Set

IGVFDS5157TBYH(CRISPR interference FACS screen targeting CD2 integrating a guide (sgRNA) library targeting transcription start sites in CD2 with low FACS signal control)

Summary

custom assembly guide quantifications

Analysis Step Version

/analysis-step-versions/886b365e-3105-424a-8972-7476238b54d7/

File Format

csv

Content Type

guide quantifications

Aliases

charles-gersbach:cd8-tcells-guide-counts-crispri-cd2

md5sum

8743026b38cc1f7c6f864c9bf5d68875

Content MD5sum

08577dbf84f62d83d3ade7bde8a32668

File Size

8.1 KB

Submitted File Name

/data/reddylab/Alex/IGVF/data_submissions/processed_data/AIM_2_T_cell_project_1/CRISPRi.CD2.counts.table.csv.gz

Checkfiles Version

Legacy version of checkfiles

Attribution

Charles Gersbach, Duke

(

HG012053 — Charles Gersbach

)

Reference Source Details

Genome Assembly

custom

File Format Specifications

1 item

Page

Description

Type

Download

Standardized File Format

Preview

gRNA counts table

file format specification

gRNA counts table file format specification.pdf

Samples

4 items

Sample Terms

Summary

IGVFSM4694MBIW

PrimaryCell

CD8-positive, alpha-beta memory T cell

Homo sapiens CD8-positive, alpha-beta memory T cell (sorting details: FACS bin for 0-10% expression of CD2 ) modified with CRISPRi Sa-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library (MOI of 0.4)

IGVFSM6350DJQS

PrimaryCell

CD8-positive, alpha-beta memory T cell

Homo sapiens CD8-positive, alpha-beta memory T cell (sorting details: FACS bin for 0-10% expression of CD2) modified with CRISPRi Sa-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library (MOI of 0.4)

IGVFSM7030KVXG

PrimaryCell

CD8-positive, alpha-beta memory T cell

Homo sapiens CD8-positive, alpha-beta memory T cell (sorting details: FACS bin for 90-100% expression of CD2) modified with CRISPRi Sa-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library (MOI of 0.4)

IGVFSM7658WDNM

PrimaryCell

CD8-positive, alpha-beta memory T cell

Homo sapiens CD8-positive, alpha-beta memory T cell (sorting details: FACS bin for 90-100% expression of CD2 ) modified with CRISPRi Sa-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library (MOI of 0.4)