HG011966

Ian Whaling

Massively parallel characterization of variants and elements impacting transcriptional regulation in dynamic cellular systems

Jay Shendure

jay-shendure

Jay Shendure, UW

Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to neuron for 19 days (cellular sub pool: 10Xlane1) modified with CRISPRa Sp-dCas9-VPH, transfected with a guide library

gRNA sequencing for scCRISPR activation screen integrating a guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci

Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to neuron for 19 days (cellular sub pool: 10Xlane2) modified with CRISPRa Sp-dCas9-VPH, transfected with a guide library

scCRISPR activation screen integrating a guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci

Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to neuron for 19 days (cellular sub pool: 10Xlane4) modified with CRISPRa Sp-dCas9-VPH, transfected with a guide library

Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to neuron for 19 days (cellular sub pool: 10Xlane3) modified with CRISPRa Sp-dCas9-VPH, transfected with a guide library

File format specification for SPECTRE tables

IGVFFI4846IRZK

differential gene expression quantifications

SCEPTRE iPSC-derived neuron hits (FDR < 0.1). Two-tailed SCEPTRE P-values were derived using default parameters. SCEPTRE P-values were then Benjamini-Hochberg corrected and those < 0.1 were kept for two-sided discovery sets.

IGVFFI4846IRZK

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