IGVFFI4132CWBF

released
validated
1{
2    "@context": "/terms/",
3    "@id": "/tabular-files/IGVFFI4132CWBF/",
4    "@type": [
5        "TabularFile",
6        "File",
7        "Item"
8    ],
9    "accession": "IGVFFI4132CWBF",
10    "aliases": [
11        "charles-gersbach:hPA-tabular-perturb-seq-grnas-set"
12    ],
13    "assay_titles": [],
14    "audit": {
15        "INTERNAL_ACTION": [
16            {
17                "category": "mismatched status",
18                "detail": "Released tabular file [IGVFFI4132CWBF](/tabular-files/IGVFFI4132CWBF/) has in progress subobject curated set [IGVFDS7723GDIK](/curated-sets/IGVFDS7723GDIK/).",
19                "level": 30,
20                "level_name": "INTERNAL_ACTION",
21                "name": "audit_item_status",
22                "path": "/tabular-files/IGVFFI4132CWBF/"
23            }
24        ]
25    },
26    "award": {
27        "@id": "/awards/HG012053/",
28        "component": "functional characterization"
29    },
30    "barcode_map_for": [],
31    "checkfiles_version": "v23",
32    "content_md5sum": "b732fd03ce917db6ea6d8eaa1e97217a",
33    "content_type": "guide RNA sequences",
34    "controlled_access": false,
35    "creation_timestamp": "2025-05-13T21:58:45.866722+00:00",
36    "derived_manually": false,
37    "file_format": "tsv",
38    "file_format_specifications": [
39        {
40            "@id": "/documents/78670549-3719-42bc-8df2-92d98918fa27/",
41            "description": "File format specification for per-guide metadata used in a CRISPR experiment",
42            "standardized_file_format": true
43        }
44    ],
45    "file_set": {
46        "@id": "/curated-sets/IGVFDS7723GDIK/",
47        "@type": [
48            "CuratedSet",
49            "FileSet",
50            "Item"
51        ],
52        "accession": "IGVFDS7723GDIK",
53        "file_set_type": "guide RNAs",
54        "status": "in progress",
55        "summary": "Homo sapiens custom guide RNAs",
56        "taxa": "Homo sapiens"
57    },
58    "file_size": 4104,
59    "gene_list_for": [],
60    "href": "/tabular-files/IGVFFI4132CWBF/@@download/IGVFFI4132CWBF.tsv.gz",
61    "input_file_for": [],
62    "integrated_in": [
63        {
64            "@id": "/construct-library-sets/IGVFDS6102AFVS/",
65            "applied_to_samples": [
66                {
67                    "@id": "/primary-cells/IGVFSM7138HWZK/",
68                    "sample_terms": [
69                        {
70                            "@id": "/sample-terms/CL_0000127/",
71                            "summary": "astrocyte",
72                            "term_name": "astrocyte"
73                        }
74                    ],
75                    "summary": "Homo sapiens astrocyte cell treated with 10 ng/mL Neurotrophin-3, 20 ng/mL Brain-Derived Neurotrophic Factor, modified with CRISPRa Sp-dCas9-2xVP64-2A-Puro, transduced (lentivirus) with a guide library"
76                },
77                {
78                    "@id": "/primary-cells/IGVFSM2166MHKA/",
79                    "sample_terms": [
80                        {
81                            "@id": "/sample-terms/CL_0000127/",
82                            "summary": "astrocyte",
83                            "term_name": "astrocyte"
84                        }
85                    ],
86                    "summary": "Homo sapiens astrocyte cell treated with 10 ng/mL Neurotrophin-3, 20 ng/mL Brain-Derived Neurotrophic Factor, modified with CRISPRa Sp-dCas9-2xVP64-2A-Puro, transduced (lentivirus) with a guide library"
87                },
88                {
89                    "@id": "/primary-cells/IGVFSM6449HHZS/",
90                    "sample_terms": [
91                        {
92                            "@id": "/sample-terms/CL_0000127/",
93                            "summary": "astrocyte",
94                            "term_name": "astrocyte"
95                        }
96                    ],
97                    "summary": "Homo sapiens astrocyte cell treated with 10 ng/mL Neurotrophin-3, 20 ng/mL Brain-Derived Neurotrophic Factor, modified with CRISPRa Sp-dCas9-2xVP64-2A-Puro, transduced (lentivirus) with a guide library"
98                }
99            ],
100            "file_set_type": "guide library",
101            "status": "released",
102            "summary": "guide (sgRNA) library targeting transcription start sites in many genes"
103        }
104    ],
105    "lab": {
106        "@id": "/labs/charles-gersbach/",
107        "title": "Charles Gersbach, Duke"
108    },
109    "loci_list_for": [],
110    "md5sum": "e3daac36e7a52744d9f05ec748c2a379",
111    "primer_design_for": [],
112    "quality_metrics": [],
113    "release_timestamp": "2025-05-16T16:07:49.388415+00:00",
114    "s3_uri": "s3://igvf-files/2025/05/13/1b6cce58-e39a-42d8-a9b3-7460f066c106/IGVFFI4132CWBF.tsv.gz",
115    "schema_version": "16",
116    "status": "released",
117    "submitted_by": {
118        "@id": "/users/3782ce69-0e9f-4b46-91f9-4578139785c7/",
119        "title": "Ruhi Rai"
120    },
121    "submitted_file_name": "/work/rr151/SamAIM2neuro_data/250512_AtN_perturbseq_metadata.tsv.gz",
122    "summary": "guide RNA sequences",
123    "upload_status": "validated",
124    "uuid": "1b6cce58-e39a-42d8-a9b3-7460f066c106"
125}