IGVFFI4074JRJB

released
validated
File Set
IGVFDS7196OWBU(CRISPR activation FACS screen targeting IL2RA: fold change over control, guide quantifications)
Summary
custom assembly guide quantifications
Analysis Step Version
File Format
csv
Content Type
guide quantifications
Aliases
charles-gersbach:jurkats-guide-counts-crispra-vp64x2-il2ra
md5sum
edb894f56207ea579bdc92c885963414
Content MD5sum
d4f38f153fb05399bca8c8ed9413d8ab
File Size
9.2 KB
Submitted File Name
/data/reddylab/Alex/IGVF/data_submissions/processed_data/AIM_2_T_cell_project_1/CRISPRa.2xVP64.IL2RA.counts.table.csv.gz

Reference Source Details

Genome Assembly
custom

File Format Specifications

Samples

6 items
Sample Terms
Summary
InVitroSystem
Jurkat cell line, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL2RA ) modified with CRISPRa Sa-dCas9-2xVP64, transduced (lentivirus) with a guide library (MOI of 0.4)
InVitroSystem
Jurkat cell line, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL2RA ) modified with CRISPRa Sa-dCas9-2xVP64, transduced (lentivirus) with a guide library (MOI of 0.4)
InVitroSystem
Jurkat cell line, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL2RA ) modified with CRISPRa Sa-dCas9-2xVP64, transduced (lentivirus) with a guide library (MOI of 0.4)
InVitroSystem
Jurkat cell line, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL2RA ) modified with CRISPRa Sa-dCas9-2xVP64, transduced (lentivirus) with a guide library (MOI of 0.4)
InVitroSystem
Jurkat cell line, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL2RA ) modified with CRISPRa Sa-dCas9-2xVP64, transduced (lentivirus) with a guide library (MOI of 0.4)
InVitroSystem
Jurkat cell line, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL2RA ) modified with CRISPRa Sa-dCas9-2xVP64, transduced (lentivirus) with a guide library (MOI of 0.4)

Files This File Derives From

6 items
bam
alignments
Charles Gersbach, Duke
17 MB
validated
bam
alignments
Charles Gersbach, Duke
15 MB
validated
bam
alignments
Charles Gersbach, Duke
20 MB
validated
bam
alignments
Charles Gersbach, Duke
19 MB
validated
bam
alignments
Charles Gersbach, Duke
17 MB
validated
bam
alignments
Charles Gersbach, Duke
18 MB
validated

Files Derived From This File

1 item
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
tsv
fold change over control
custom assembly fold change over control
Charles Gersbach, Duke
15 KB
validated

Attribution