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IGVFFI3072IFRD

released
validated
File Set
IGVFDS5157TBYH(CRISPR interference FACS screen targeting CD2: fold change over control, guide quantifications)
Summary
custom assembly fold change over control
Analysis Step Version
/analysis-step-versions/6a1d20c7-c92f-47d6-8e3b-5c74b3e8eed2/
File Format
tsv
Content Type
fold change over control
Aliases
charles-gersbach:cd8-tcells-guide-quantifications-crispri-cd2
md5sum
f996fcfc9388fdb829c6f3a6416ce1f4
Content MD5sum
f90a915b59c630c50fefce0547f2d985
File Size
13 KB
Submitted File Name
/data/reddylab/Alex/IGVF/data_submissions/processed_data/AIM_2_T_cell_project_1/T1A_CD2_CRISPRi_screen.simple.v2.txt.gz

Reference Source Details

Genome Assembly
custom

File Format Specifications

Samples

4 items
Sample Terms
Summary
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of CD2 ) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of CD2) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of CD2) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of CD2 ) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)

Files This File Derives From

1 item
Accession
File Set
File Format
Content Type
Lab
File Size
Upload Status
csv
guide quantifications
Charles Gersbach, Duke
8.1 KB
validated

Attribution

Award
HG012053
Principal Investigator(s)
Contact P.I.
Charles Gersbach
Lab
Charles Gersbach, Duke