IGVFFI1466VTFO

released
validated
File Set
IGVFDS3778BYII(Homo sapiens GRCh38 custom guide RNAs)
Summary
GRCh38 guide RNA sequences
File Format
tsv
Content Type
guide RNA sequences
Aliases
charles-gersbach:cd8-tcell-b2m-grnas-metadata
md5sum
6bf311403b0c4dbaeddecacbb83733ca
Content MD5sum
82bbcce85b86c0b98bd14367dcb540c8
File Size
7.2 KB
Submitted File Name
/data/reddylab/Alex/IGVF/data_submissions/reference_data/AIM_2_T_cell_project_1/B2M.screen.igvf_metadata.v3.txt.gz

Reference Source Details

Genome Assembly
GRCh38

Samples

6 items
Sample Terms
Summary
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of b2m) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of b2m) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of b2m) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of b2m) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)

Integrated In

1 item
Accession
Type
Summary
Aliases
Lab
ConstructLibrarySet
guide (sgRNA) library targeting transcription start sites in B2M
  • charles-gersbach:cd8-tcell-b2m-grna-construct-lib-set
Charles Gersbach, Duke

Attribution