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IGVFFI1405WMSH

revoked
validated
File Set
IGVFDS4729GLXH(Homo sapiens GRCh38 guide RNAs)
Summary
GRCh38 guide RNA sequences
File Format
tsv
Content Type
guide RNA sequences
Aliases
charles-gersbach:cd8-tcells-perturb-seq-grnas-metadata
md5sum
f274e5639a730e070638d0facf569306
Content MD5sum
1204d5b7f6ad2c94b3cca031b61eba54
File Size
1.4 KB
Submitted File Name
/data/reddylab/Alex/IGVF/data_submissions/reference_data/AIM_2_T_cell_project_1/TF_CRISPR_i_a.scRNAseq_screen.igvf_metadata.v2.txt.gz
Checkfiles Version
Legacy version of checkfiles
Revoke Detail
This has been revoked due to not including the guide_id. The updated version can be found here: https://data.igvf.org/charles-gersbach:cd8-tcells-perturb-seq-grnas-metadata-v3/

Reference Source Details

Genome Assembly
GRCh38

Samples

6 items
Sample Terms
Summary
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)

Integrated In

1 item
Accession
Type
Summary
Aliases
Lab
ConstructLibrarySet
guide (sgRNA) library targeting transcription start sites in 24 genes
  • charles-gersbach:cd8-tcell-perturb-seq-crispr-grna-construct-lib-set
Charles Gersbach, Duke

Attribution

Award
HG012053
Principal Investigator(s)
Contact P.I.
Charles Gersbach
Lab
Charles Gersbach, Duke