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IGVFFI0520IBDF

released
validated
File Set
IGVFDS8839AFQB(CRISPR interference FACS screen targeting B2M: fold change over control, guide quantifications)
Summary
custom assembly guide quantifications
Analysis Step Version
/analysis-step-versions/886b365e-3105-424a-8972-7476238b54d7/
File Format
csv
Content Type
guide quantifications
Aliases
charles-gersbach:cd8-tcells-guide-counts-crispri-b2m
md5sum
1dfe9711461e9bba2da55d6020feaced
Content MD5sum
627151ec76e10f97cf9b93d79ebd71da
File Size
11 KB
Submitted File Name
/data/reddylab/Alex/IGVF/data_submissions/processed_data/AIM_2_T_cell_project_1/CRISPRi.B2M.counts.table.csv.gz

Reference Source Details

Genome Assembly
custom

File Format Specifications

Samples

6 items
Sample Terms
Summary
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of b2m) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of b2m) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of b2m) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of b2m) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of b2m) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of b2m) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)

Files This File Derives From

6 items
bam
alignments
Charles Gersbach, Duke
14 MB
validated
bam
alignments
Charles Gersbach, Duke
16 MB
validated
bam
alignments
Charles Gersbach, Duke
13 MB
validated
bam
alignments
Charles Gersbach, Duke
16 MB
validated
bam
alignments
Charles Gersbach, Duke
14 MB
validated
bam
alignments
Charles Gersbach, Duke
5.1 MB
validated

Files Derived From This File

1 item
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
tsv
fold change over control
custom assembly fold change over control
Charles Gersbach, Duke
17 KB
validated

Attribution

Award
HG012053
Principal Investigator(s)
Contact P.I.
Charles Gersbach
Lab
Charles Gersbach, Duke